cryo-EM

The paper introduces a method to calculate variable-resolution maps using analytic shell-function approximations to atomic images, enabling comparison with experimental cryo-EM maps.

This study uses cryo-EM and molecular dynamics to analyze conformational transitions in AE1 and shows how PIP2 lipids inhibit transport by stabilizing the outward-facing state.

The paper reports the cryoEM structure of TraT, an outer membrane protein involved in plasmid exclusion, and suggests it functions by physical interference rather than specific protein interactions.

This study identifies a tyrosination-dependent mechanism that enables dynein-2 to specifically bind the A-tubule of ciliary doublet microtubules, facilitating retrograde IFT.

The paper investigates the structural differences between two capsid sizes in bacteriophage P1, revealing the role of protein appendages in DNA packaging and capsid morphogenesis.

This paper uses molecular dynamics simulations at 310 K to analyze cryo-EM reconstructions of actin filaments, revealing conformational differences from physiological conditions and explaining functional properties.

The paper uses cryo-EM and simulations to reveal how LptM and LptY facilitate LPS transport by modulating LptD conformational dynamics in the translocon complex.

This study presents the cryo-EM structure of a bacterial Type VIIb secretion system core complex, showing how a ubiquitin-like protein coordinates its assembly and function.

This paper introduces a method to achieve microsecond time resolution in cryo-EM using jet vitrification, enabling observation of protein dynamics with near-atomic resolution.

SPITROBOT-2 improves cryo-trapping crystallography with a 23 ms delay, reducing sample preparation time to three minutes per sample.

The paper reveals the closed state structure of the Shaker K+ channel by uncoupling voltage sensor domains, showing a novel 'roll and turn' movement of S6 helices and a noncanonical selectivity filter state.

The paper identifies ADGRL2's role in epidermal differentiation through Gα13 activation and structural interactions, using CRISPR, Perturb-seq, and cryo-EM.

The paper uses energetic profiling on cryo-EM data to uncover thermodynamic principles governing amyloid fibril maturation and polymorphic divergence.

This study identifies MAP9 as a microtubule stabilizer that promotes neuronal morphogenesis and differentially regulates kinesin motor activity.

This paper develops a minimal energy-landscape model to explain how mechanical force affects T-cell activation by reshaping activation barriers and bond lifetimes through catch-slip dynamics.

This study reveals the mechanism by which the 26S proteasome recognizes and degrades ODC without ubiquitin, through cryo-EM structures showing dynamic interactions and translocation processes.

This study investigates the structural and biophysical properties of E. coli Dps across a pH range, revealing pH-dependent DNA binding and assembly behavior.

The paper determines the cryo-EM structure of a Shaker K+ channel mutant and shows that voltage sensing domain activation induces a dynamic shift in the linker population equilibrium rather than directly pulling the gate open.

The paper introduces MISO, a method combining microfluidics and cryo-EM to determine protein structures from single cell colonies with high sensitivity.

This paper introduces a deep learning method to improve single particle cryoEM data quality by automatically filtering noisy images using denoising and domain adaptation neural networks.

The paper investigates two LarA-like enzymes lacking a catalytic histidine residue, solving their structures and revealing a novel octameric assembly and active site residues involved in carbohydrate metabolism.

This study determines cryoEM structures of human separase in apo and substrate-bound forms, revealing substrate recognition mechanisms and cleavage regulation.

CryoLike evaluates image-to-structure likelihoods for cryo-EM data using Fourier-Bessel representations to improve computational efficiency.

This paper presents cryo-EM structures of PORCN with inhibitors, revealing a conserved water-mediated binding mechanism for rational drug design.

This study identifies structural mechanisms of gp130 mutant activation in IHCAs using cryo-EM and mutagenesis, revealing two distinct dimer conformations and their activation dependencies.

This study reveals the molecular mechanism of Retron-Eco8 immunity by determining cryo-EM structures of the system in inactive and activated states, showing how phage SSB triggers nuclease activation and cell death.

The paper presents cryo-EM structures of Mycobacterium tuberculosis pyruvate carboxylase, revealing how acetyl-CoA stabilizes the enzyme and enables domain coordination during catalysis.

The paper reports cryo-EM structures of PfNCR1 bound to four inhibitors, revealing distinct binding modes that inform drug discovery targeting this malaria parasite transporter.

The paper determines the structure of VPS13C using cryo-EM, revealing a lipid-transfer-nonpermissive conformation and identifying calmodulin as a regulatory partner.

This paper evaluates blind predictions of dynamic water and ion ensembles around RNA using cryo-EM densities from CASP16, finding that molecular dynamics approaches perform best.