cryo-EM

This study determines the structural and kinetic mechanism of DNA Polymerase β processing 1-nt gaps in the nucleosome during DNA repair.

The paper identifies a novel phosphorylated NES in Pho4 recognized by Msn5, revealing a phosphate-specific nuclear export mechanism in yeast.

The paper uses cell-free assays to show PACRG and FAP20 synergistically stabilize microtubule doublets by reducing B-tubule depolymerization and increasing rescue frequency.

This study uses shotgun-EM to identify and characterize three protein complexes, including the first experimentally determined structure of Pks16 in D. discoideum.

This study determines the structures of 30S initiation complexes inhibited by kasugamycin, edeine, and GE81112, revealing distinct mechanisms of action within the E-site.

The paper identifies a shared mechanism between Bacteroidota gliding motility and T9SS protein transport involving disulfide-bonded outer membrane proteins.

This study uses cryo-EM and deep learning to analyze conformational changes in ribonucleotide reductase during turnover, revealing dynamic asymmetry and transient states essential for catalysis.

The paper uses cryo-electron tomography to reveal the in situ architecture of the polar tube invasion apparatus in microsporidian parasites.

Two Tetrahymena ribozyme cryo-EM maps (2.2 and 2.3 Å) reveal cross-validated ordered waters and Mg2+ ions, plus reproducible diffuse solvent density resembling molecular dynamics water networks.

This study determines the structure of the Ebola virus nucleocapsid using cryo-EM and identifies VP24's role in assembly and function.

This paper introduces SISTM to map 3D synaptic nanoarchitecture in brain tissue across species using cryo-ET and STA, revealing conserved structural features in human postmortem tissue.

This study elucidates the molecular mechanism of redox-coupled sodium pumping in the Rnf complex using cryo-EM, biochemical assays, and simulations.

This paper demonstrates xenon plasma focused ion beam milling for preparing high-pressure frozen specimens, achieving a 4.0 Å E. coli ribosome structure with minimal structural damage beyond 45 nm.

The paper uses cryo-EM to study how G4 DNA structures stall replisomes and reveals an inchworm mechanism for DNA translocation.

The paper identifies inconsistencies in rabbit ribosomal rRNA sequences and proposes using the Broad Institute's genome sequence to standardize numbering and improve model consistency.

This study presents cryo-EM structures of Nipah virus RdRp in apo and early elongation states, revealing its architecture and RNA interaction mechanisms.

The paper determines the structures of tau filaments in individuals with MAPT mutations V337M and R406W using cryo-EM, finding the Alzheimer fold in all cases and a new triple filament assembly.

This study uses cryo-ET to visualize copia retrotransposon structure in Drosophila cells, revealing nuclear clustering and size selection during gametogenesis.

This study determines the high-resolution structure of the ciliary glycocalyx in Chlamydomonas reinhardtii and identifies FMG1B as a mucin-like protein with unique glycosylation features.

This study shows phosphorylation at specific residues on stressosome proteins dictates assembly, activation, and function in Listeria monocytogenes, with implications for virulence and antimicrobial targets.

This study determines the structure of the CODANIN-1_ASF1 complex, revealing how CODANIN-1 inhibits ASF1-mediated histone supply by sequestering ASF1 in the cytoplasm.

This study reveals how MIDN enhances proteasome activity through cryo-EM structures showing its binding to proteasomes and substrates in two conformational states.

This paper presents the cryo-EM structure of the RC-LH1 supercomplex from Dinoroseobacter shibae, revealing structural adaptations for anaerobic anoxygenic photosynthesis in oxygen-rich environments.

This paper introduces a method for purifying HIV virions with high yield and minimal force, enabling detailed structural analysis of virion maturation.

The paper uses cryo-EM, molecular dynamics, and small-angle neutron scattering to characterize an open and closed state of the bacterial ion channel DeCLIC under acidic conditions, revealing structural and dynamic mechanisms of gating.

This paper uses cryo-EM to solve high-resolution structures of actin-bound myo1c with and without ADP, revealing unique actin-binding orientation, ADP release pathway, and power stroke trajectory.

The paper determines the cryoEM structures of MUC5AC variants and shows their structural basis for mucus formation and disease risk in COPD and IPF.

This study reveals how lipid competition modulates drug affinity in ABC exporters through structural and simulation analyses of BmrCD.

The paper determines the cryoEM structure of the Arabidopsis R2T complex, revealing its dodecameric organization and distinct architecture compared to the human R2TP complex.

The paper presents a cryoEM structure of the human THIK-1 K2P K+ channel, revealing structural features and regulatory mechanisms involving lipids and anaesthetics.