cryo-EM

This study presents cryo-EM structures of sHSPs in apo and client-bound states, revealing mechanisms of client sequestration and polydispersity in sHSP complexes.

This paper presents a streamlined method for (an)aerobic vitrification of oxygen-sensitive proteins using an automated device, enabling high-resolution cryoEM structure determination.

This study uses cryo-EM to determine high-resolution structures of type I and IV pili from enterotoxigenic Escherichia coli, revealing subunit interactions and hydrated water molecules.

This paper uses time-resolved cryoEM to directly observe the swinging lever mechanism of myosin-5, showing its structural changes during ATP hydrolysis and force generation.

This study presents the first structural description of the fusobacterial TAA CbpF and its interaction with CEACAM1, revealing a novel binding mode and potential targets for drug design in CRC.

This paper investigates fusion-intermediate conformations of SARS-CoV-2 spike by using an HR2 peptide inhibitor, showing that receptor binding and cleavage at S1/S2 or S2' sites are required to capture these intermediates.

The paper uses structural biology techniques to study how nascent polypeptide chains fold through two distinct pathways during ribosome-mediated translation.

The paper determines how the human 26S proteasome distinguishes between K48-linked and K11/K48-branched ubiquitin chains through structural analysis and functional studies.

The paper identifies receptor-binding polymorphisms in WEEV that explain shifts in receptor dependencies and enable nonpathogenic strains to infect neurons.

DeepCryoRNA is a deep learning method for reconstructing RNA 3D structures from cryo-EM maps, showing higher accuracy than existing methods on a benchmark dataset.

This study uses cryo-EM to visualize how SNF2H, a chromatin remodeler, translocates DNA around the histone octamer by inducing conformational changes.

This study determines the structure of Drp1 on lipid membranes, revealing a locally ordered lattice and conformational changes that suggest a ratchet mechanism for mitochondrial constriction.

This study determines the structure of the AP5:SPG11-SPG15 complex and shows it binds PI3P, senses membrane curvature, and drives membrane remodeling in vitro.

This paper defines neutralizing epitopes on pertussis toxin using cryo-EM structures and functional assays, providing insights for vaccine development.

This study presents cryo-EM structures of GAS2 domains bound to actin and microtubules, revealing their roles in cytoskeletal organization and dimerization.

This study presents cryo-EM structures of hURAT1 bound with anti-gout drugs and uric acid, revealing transport mechanisms and drug inhibition.

This study shows DNAJB1 recruits Hsc70 in two steps to amyloid fibers, forming ordered arrays that enable efficient disaggregation, while a mutant disrupts this organization.

This study uses cryo-EM to show AKAP350 forms fibrillar clusters and interacts with DNA and DNA-binding proteins like NFIB and NCL.

The paper warns that counting particle images in cryo-EM can lead to biased estimates of conformational state populations due to noise and orientation uncertainty, and suggests using full image datasets for more accurate modeling.

This study characterizes the structure of Turnip yellows virus particles using cryo-EM and immunogold labelling, showing that RTP* insertion is random and does not disrupt capsid structure.

This study uses cryo-EM to analyze actinoporin pores and shows how lipids interact with the protein, revealing their role in pore stability and structure.

This study shows that glutamate gating of AMPA receptors is temperature sensitive and distinct from PAM-induced gating, revealing conserved structural motifs and desensitization mechanisms.

This study reveals how XPR1, a phosphate exporter, is regulated by KIDINS220 and inositol pyrophosphate through structural and functional analyses.

The paper determines the structure of ARF6 protein lattice on tubulated membranes, revealing its role in vesicle fission and how GAP proteins interact with it.

The paper identifies the Hitchdock domain in kinesin-2 tail as critical for adaptor assembly and cargo binding, revealing its structural role in interactions with KAP3 and APC.

This study determines the molecular organisation of the human prohibitin complex within the mitochondrial inner membrane using structural biology methods.

The paper determines the structure of mycobacterial NDH-2 bound to an inhibitor using cryo-EM, revealing structural features that could guide inhibitor design.

This study uses cryo-EM to visualize the dynamics of DNA unwinding by the SV40 Large Tumor Antigen helicase, revealing how ATP hydrolysis drives translocation and strand separation.

The paper uses cryogenic electron tomography to reveal a stargate mechanism for Microviridae genome delivery, showing it is universal across the family.

The paper presents cryo-EM structures of the human taurine transporter, identifies taurine-binding sites, and shows that enhanced taurine uptake reduces cellular senescence in biliary epithelial cells.