cryo-EM

The paper improves modeling of cryo-EM maps for membrane proteins with multiple states using generative AI and density-guided simulations.

This study reveals how mycobacterial HflX mediates ribosome splitting and antibiotic resistance through rRNA disordering and anti-association mechanisms.

This study maps the structural pathway of class III PI 3-kinase activation by showing how N-myristoylation of VPS15 stabilizes the inactive conformation and how GTP binding promotes activation.

This study presents the highest resolution cryo-EM structure of a BAR protein and shows endophilin B1 permeabilizes liposomes containing cardiolipin, proposing a new model for Bax-mediated cell death.

The paper presents a cryo-EM structure of the M5 mAChR with a novel allosteric binding site identified through mutagenesis, pharmacology, and simulations.

The paper uses cryoEM and machine learning to identify and classify novel oligomeric protein complexes from Azotobacter vinelandii, revealing new structural states of proteins like phosphoglucoisomerase and pyridine transhydrogenase.

The paper presents an in silico method to classify and identify co-purified protein complexes using cryoEM data, revealing new structures and multiple membrane scaffold protein assembly states.

The paper designs collagenous assemblies using a peptide self-assembly system and solves their structure with cryo-EM, revealing a non-twisting triple helix conformation that differs from the canonical right-handed triple helix.

The paper presents high-resolution cryo-EM structures of aerolysin pre-pore and pore in a membrane-like environment, revealing key interactions and constriction rings relevant for nanopore sensing.

The paper trains segmentation networks for cryo-ET data using synthetic and real data, showing that synthetic data with molecular diversity improves performance but real data is needed for generalization.

The paper reports the cryo-EM structure of H5N1 hemagglutinin showing an auto-glycan from N169 binds the receptor-binding site, highlighting auto-glycan recognition in zoonotic transmission.

The paper determines Cryo-EM structures of the EDEM:PDI complex with and without a substrate, revealing how disulfide bonds regulate conformational changes essential for substrate engagement.

The paper uses cryo-EM to determine the structure of bacteriophage 7-7-1 and shows it uses capsid fibers for initial host attachment.

The paper develops a rapid in situ cryo-ET workflow for bacterial ribosome structure analysis, achieving sub-nm resolution in E. coli within 10 days.

This paper presents a 2.85 Å cryo-EM structure of MMOH-MMOB, revealing conformational changes and Fe-Fe distances critical for oxygen activation in methane monooxygenase.

This study determines high-resolution structures of HIV-2 CA lattice using cryo-EM, revealing structural features and differences in host factor interactions compared to HIV-1.

The paper reports cryo-EM structures of Salmonella typhimurium ArnC in apo and UDP-bound forms, revealing its tetrameric structure and conformational changes upon UDP binding.

This study determines the cryo-EM structures of human myoferlin and dysferlin bound to Ca2+ and lipids, revealing how their domain dynamics enable membrane binding and fusion.

This paper uses cryo-EM to determine the structure of Rad51-ssDNA filaments in budding yeast, revealing local structural dynamics and conformational changes in Rad51 protomers.

This study uses cryo-EM to analyze the structural changes in C3 during hydrolysis, revealing a dynamic opening in the macroglobulin ring that allows domain translocation.

This study reveals the in-situ architecture of the mammalian pyruvate dehydrogenase complex using cryo-electron tomography, showing dynamic interactions and variable peripheral compositions.

This paper presents a scaffold strategy to improve cryo-EM resolution for small RNAs, demonstrating high-resolution structures of a TPP riboswitch and its ligand binding dynamics.

This paper identifies an unknown contaminant in protein samples using Cryo-EM and AI tools, leading to improved purification protocols.

This study determines the cryo-EM structure of the Nipah virus polymerase complex and identifies key domains critical for replication and transcription.

This paper introduces a cryo-electron tomography pipeline for imaging plasma membranes with angstrom-scale resolution and targeted protein labeling.

The paper reports cryo-EM structures of aberrant spliceosome intermediates in S. pombe, revealing how a helicase-protein pair maintains catalytic dormancy and facilitates disassembly.

This study investigates how fascin's structural plasticity enables flexible actin bundle formation through cryo-EM, tomography, and modeling.

The paper identifies the binding site of small molecule inhibitors NBA and IBA in TRPM4 using cryo-EM and patch clamp experiments.

The paper identifies a novel non-motile dynein heavy chain subfamily, DNAHX, using cryo-EM and shows its evolutionary origin and structural features.

The paper reports Cryo-EM structures of AAV2 Rep68 bound to DNA at the AAVS1 integration site, revealing conformational changes and DNA distortion mechanisms during replication.