cryo-EM

The paper characterizes human MCM double hexamer (hDH) loading using biochemical reconstitution and cryo-EM, revealing differences from yeast in assembly mechanisms and identifying a novel intermediate.

This study uses cryo-electron tomography to describe the molecular architecture and structural heterogeneity of individual synaptic vesicles in mouse brains and cultured neurons.

This study uses cryoID to reconstruct the native PLP synthase complex from Methanosarcina acetivorans lysate, revealing a homo-dodecamer structure and absence of a previously proposed supracomplex.

This study determines the structure of the Retriever-SNX17 complex using cryo-EM, revealing how SNX17 interacts with Retriever to facilitate endosomal sorting of cargo proteins.

This study uses cryo-ET and AI-based methods to analyze thylakoid membrane organization in spinach chloroplasts, revealing similarities to green algae despite differences in 3D architecture.

DomainSeeker identifies protein domains in cryo-ET maps using AlphaFold2 structures and Bayesian inference, improving accuracy and resolution robustness.

The paper reports a cryo-EM structure of the HRSL domain from GCN2, revealing its role in activating the kinase and regulating the ISR pathway.

This study uses cryo-EM to determine high-resolution structures of apo-APC/C and APC/C CDH1:EMI1, revealing new structural features and regulatory mechanisms.

This paper introduces spIsoNet, a deep-learning tool that improves 3D reconstruction isotropy and particle alignment in cryoEM by addressing the preferred orientation problem without altering specimen preparation.

The paper reveals the cryo-EM structure of hTAS2R14 bound to FFA and gustducin, showing dual binding pockets and supporting a dual binding mode for FFA.

This study reveals a novel pentameric assembly of tail tube protein in SPR phages using cryo-EM, showing structural features that influence tube curvature and genome release.

The paper analyzes SARS-CoV-2 variant virion morphology and spike protein structures using cryo-EM, finding no adaptive changes in virion shape but multiple amino acid changes affecting spike conformational dynamics.

This study presents the cryo-EM structure of the spinach plastid-encoded RNA polymerase complex, revealing its composition and spatial arrangement of subunits and associated proteins.

The paper shows that laser phase-plate images of apoF and rubisco reveal unexpected structural heterogeneity, suggesting that specimen damage may be more prevalent than previously thought.

The paper introduces a Python package to map protein conformational landscapes using crystallographic drug fragment screens and applies it to PTP-1B and SARS-CoV-2 MPro.

This study examines the aggregation dynamics of a 150 kDa Aβ42 oligomer using cryo-EM and other techniques, revealing unique string-like assemblies distinct from classical fibrils.

This study uses cryo-EM and smFRET to reveal structural and kinetic details of yeast TFIIIC engagement with tRNA promoter DNA motifs.

This study investigates how HURP regulates Kif18A motility and microtubule dynamics during mitosis using single-molecule imaging and Cryo-EM.

The paper presents cryo-EM structures of the mitochondrial RNase Z complex bound to tRNAHis, revealing mechanisms of tRNA maturation and linking ELAC2 mutations to mitochondrial diseases.

This study uses cryo-EM and genetic engineering to show how USP14 non-catalytically regulates proteasome activity through salt-dependent allosteric changes.

The paper determines the cryo-EM structure of the UBA7-UBE2L6 disulfide complex and identifies factors influencing its formation, revealing its role in ISG15 conjugation and immune response regulation.

This study uses cryo-electron tomography to show COPII forms spherical vesicles by binding cargo on native membranes, revealing its structure and how it generates membrane curvature.

The paper determines the structure of a novel α-synuclein filament fold in multiple system atrophy using cryo-EM, identifying a new filament type distinct from other synucleinopathies.

TomoScore assesses cryo-ET tomogram quality for cellular annotation using a deep learning model, identifying optimal electron dose ranges to improve data processing efficiency.

This study determines the structure of sensor-activated NbNRC2 using cryo-EM, revealing a hexameric resistosome and highlighting structural changes during NLR activation.

The paper introduces a phantom sample and annotated 3D volumes for a machine learning challenge to improve cryoET volume annotation.

The paper reports the cryo-EM structure of TRPC1/5 heterotetramer, revealing structural features that differentiate it from TRPC5 homomers and provide insights into its functional properties.

The paper solves the cryo-EM structure of CCR1-Gi complex in pre-coupled state, revealing molecular mechanism of GPCR-G protein activation and highlighting conserved pre-coupling in chemokine receptors.

This study identifies polyphosphate as the 'mystery density' in α-synuclein fibrils by showing it binds to lysine residues, affecting fibril formation and toxicity.

The paper introduces cryoMDM, a method that estimates 3D atomic models from single cryoEM particle images by matching them to simulated structural models, revealing intermediate structures of the SARS-CoV-2 spike protein.