cryo-EM

This study characterizes IDE conformational dynamics using cryo-EM and MD simulations, identifying key residues and mechanisms that modulate enzyme activity.

This study investigates nearest-neighbor cooperativity in TRAP by engineering dTRAP variants and using cryo-EM and thermodynamic analyses to reveal structural and thermodynamic mechanisms of ligand binding cooperativity.

The paper determines the structure of the Na+-driven stator PomAB from Vibrio using cryo-EM and reveals the sodium ion pathway, ion selectivity mechanism, and inhibition by phenamil.

The paper reports a cryo-EM structure of Chlamydomonas mastigoneme, revealing a 5',5'-phosphodiester bond stabilizing glycan folding.

The paper determines the structure of siphophage T5 tail terminator protein TrP142 and tail completion protein TCPp143 using cryo-EM and Alphafold2, revealing their localization and structural features.

This study uses cryo-EM to visualize how dantrolene inhibits the Y522S RyR1 mutant, revealing its binding site and mechanism of action in preventing malignant hyperthermia.

This study identifies a second drug-binding site in P2X3 receptors and shows camlipixant acts as an allosteric inhibitor, providing insights into its selectivity and mechanism of action.

The paper describes the structure and immunogenicity of a stabilized CCHFV heterotrimeric glycoprotein complex and shows it provides 40% protection against viral challenge in mice.

This paper presents three structures of PfNCR1, a Plasmodium falciparum membrane protein, and shows it exports cholesterol to maintain membrane homeostasis, with MMV009108 inhibiting this function.

This paper provides a protocol for high-resolution cryo-EM structure determination of α-synuclein fibrils, resulting in a 2.04 Å structure with new density features.

This paper introduces a new statistical metric, 2DTM p-value, to improve target detection in cryo-EM by combining SNR and z-score, showing better performance for complex targets.

Cryo-ET of 169 nm β-carboxysomes from low-light Synechococcus elongatus reveals ~639 Rubiscos in concentric layers with CcmM bound at RbcL grooves; QconCAT proteomics yields the complete absolute protein stoichiometry.

The paper discovers a new glycofibril structure using CryoSeek, revealing its 8-nm diameter and unique glycan-coated tetrapeptide repeats.

The paper reports cryo-EM structures of PSI-FCPI complexes from Thalassiosira pseudonana under high light, revealing light-adaptive strategies and convergent evolution in diatom FCPIs.

This study reports the 3.2 Å resolution structure of the Pseudomonas aeruginosa PAO1 Type IV pilus using cryo-EM, revealing its architecture and potential functional roles.

The paper determines the structures of three ENaC complexes, revealing how subunit composition affects channel function through structural rearrangements.

This study determines the structure of apoB100 from human LDL using cryo-EM, AlphaFold2, and molecular dynamics, revealing its structural organization and functional domains.

The paper identifies a contaminant phage from a cryoEM grid and determines its tail structure using ML-based methods without prior sequence knowledge.

The paper evaluates crystal diffraction data quality metrics, finding that low-shell values of Rmerg, Rmeas, Rpim, and CC1/2 indicate Laue symmetry correctness, while high-shell I/σI is better for resolution cutoff selection.

This study analyzes the factors affecting resolution in cryoET-STA for MmCpn, evaluating the cost-benefit of achieving high-resolution structures.

This study presents cryo-EM structures of human NCX1 with PIP2 and SEA0400, revealing how these molecules modulate NCX1 activity through conformational changes.

The paper determines the cryoEM structure of the Ric1-Rgp1-Rab6 complex, revealing its mechanism for Rab6 activation and identifying a novel RabGEF domain and arrestin fold interactions.

This study uses cryo-EM to show that the W328-6H2 antibody forms different structures with SARS and Omicron BA.1 spike proteins, revealing a supplementary neutralization mechanism beyond RBD-Fab binding.

The paper presents a high-resolution structure of the ciliary tip central pair in Tetrahymena thermophila and identifies proteins that stabilize microtubules.

The paper presents cryo-EM structures of the FDX2-bound core ISC complex, revealing two-stage binding of FDX2 and its role in electron transfer during [2Fe-2S] cluster biosynthesis.

This study uses cryo-EM and single-molecule FRET to show conformational coupling between the extracellular and transmembrane domains of an aGPCR, revealing how ECR dynamics influence receptor function.

The paper determines the structure of yeast RAVE bound to a partial V1 complex using cryo-EM, revealing its role in facilitating V1-VO reassociation.

The paper presents cryo-EM structures of human mitochondrial transcription initiation complexes, revealing mechanisms of start-site selection and slippage synthesis.

This study presents cryo-EM structures of MCHR1 in active and inactive states, revealing ligand recognition and activation mechanisms to aid drug development.

The paper investigates the structural dynamics of αB-crystallin by mutating the NT-IXI motif, revealing how this affects fibrillar assembly and chaperone function using cryo-EM.