imaging

Differential Survival of Retinal Ganglion Cell Types Following Branch Retinal Vein Occlusion

In a mouse branch retinal vein occlusion model, retinal ganglion cells die progressively over 14 days. α-RGCs and F-RGCs are lost fastest, and TBR2-positive intrinsically photosensitive RGCs survive best.

Differential and regionally distinct molecular responses distinguish FLASH and conventional RT in a medulloblastoma model while preserving therapeutic outcomes

Hypo-fractionated FLASH and conventional radiotherapy (3x10 Gy) each ablated orthotopic human medulloblastoma in nude mice; FLASH evoked distinct microglial and hippocampal transcriptional programs and less neuroinflammation.

Expression of tardigrade intrinsically disordered proteins in C. elegans reveals variable effects on aging and stress

Constitutive somatic expression of four tardigrade disordered proteins shortened C. elegans lifespan and healthspan and gave little stress protection. Three of the four reduced polyQ aggregation under hyperosmotic stress.

Estimating glioblastoma migration-to-proliferation ratio from single time-point MRI data via radial density distribution

A reaction-diffusion model fitted to shell-averaged tumor density from one pre-treatment MRI estimates glioblastoma migration/proliferation ratio (D/k); in BraTS 2017, higher D/k went with shorter overall survival.

Reduced cerebral blood flow and metabolic rate trigger an adaptive mitochondrial remodeling program in a Down syndrome mouse model

In Dp16 Down syndrome mice, MRI shows reduced cerebral blood flow and oxygen metabolism (significant in males), while brain mitochondrial respiratory capacity stays largely intact and oxidative phosphorylation genes rise.

Fss1, a CTNS/PQ domain protein, has dual roles in promoting fertility in Schizosaccharomyces pombe

Fission yeast Fss1, a PQ-loop membrane protein, acts in diploids to build full-thickness, chitin-containing spore walls and within spores to maintain vacuole morphology and timely germination.

A human induced pluripotent stem cell-based high-throughput screening platform for teratogenicity hazard identification

Four-model hiPSC screen in 384-well imaging reached 83% accuracy (88% sensitivity, 75% specificity) on 130 scored compounds from a 146-compound reference set, scalable to hundreds of compounds monthly.

Linking clinical border morphology to cell-scale growth mechanisms in melanoma: a segmentation-to-simulation alignment study

Across 1,581 ISIC 2018 lesions, melanoma borders differ from nevi mainly by coarse lobulation rather than fractal dimension; of six simulated mechanisms, only an imposed long-range growth-rate field reproduces this.

Accelerating drug development in infectious diseases using zebrafish disease models supported by pharmacokinetic pharmacodynamic modeling as new approach methodology (NAM)

Zebrafish larva infection experiments plus pharmacokinetic-pharmacodynamic (PKPD) modelling gave bedaquiline's in vivo Emax and EC50 against M. marinum in about 2 months; waterborne clarithromycin barely entered larvae.

Whole-body single-cell atlas of an adult vertebrate in homeostasis and regeneration

Region-stratified scRNA-seq of 607,370 cells plus 500-gene MERFISH maps adult Danionella cerebrum cell types body-wide, revealing fibroblast Hox and limb-identity programs and telencephalon regeneration dynamics.

The epidermal growth factor (EGF) receptor family network in cardiac hypertrophy and heart failure

EGFR-family ligand mRNAs shift in failing human hearts; NRG1 and EGF drive distinct, differently ERBB2-dependent transcriptional programmes in rat cardiomyocytes; afatinib blunts mouse angiotensin II hypertrophy but worsens fibrosis.

Host response against SARS-CoV-2 infection is impacted by allosteric modulation of cholinergic and purinergic receptors

Ivermectin's protection in SARS-CoV-2-infected hamsters depended partly on both α7 nicotinic and P2X4 receptors; in co-cultures its effects were α7nAChR-independent, and P2X4 blockade prevented CXCL10 induction at 48 hpi.

A clinically annotated proteomic and histological resource of 1,000 colorectal cancers

Deep proteomics of 997 treatment-naïve colorectal cancers defines six subgroups, splitting chromosomally unstable tumors into three and revealing a rare adverse subgroup, with H&E slide benchmarks.

2C-RT Quantifies Genome-Wide DNA Replication Perturbations and Redefines RIF1-CDC7 Regulation of Replication Timing

Sorting S-phase cells by Cyclin A2 rather than DNA content exposes a uniform genome-wide delay after CDC7 inhibition and a broad replication timing advance in RIF1 knockout.

MYC targeted de novo binders impair tumor growth

Designed protein binders to conserved MYC regions reach low-nanomolar affinity, reduce MYC-MAX proximity in cells, and are depleted from MYC-dependent cancer cells in culture and mouse pancreatic tumors.

gene2fish: a multi-gene spatial expression browser for in situ hybridization data across zebrafish development

gene2fish arranges 53,595 Thisse in situ hybridization images of 8,263 zebrafish genes into a gene-by-stage grid, with anatomy-term search for validating scRNA-seq markers.

Neural signatures of glioma progression using implanted neural interfaces

Microwire recordings in glioma-bearing mice track tumor progression: gamma power rises in glioblastoma and diffuse intrinsic pontine glioma xenografts, and low-gamma trajectories predict tumor growth rate (R^2 0.63).

The modular nature of long-association pathways in the human brain

Diffusion MRI tractography below 750 µm resolves the dorsal superior longitudinal fasciculus into layered fiber systems of varying length, matching macaque tracing; 1.5 mm tractography misses this.

Heterogeneity in cilia patterning enables multiple flow functions in Paramecium

Paramecium tetraurelia partitions its ciliary array into a dense, fast-beating domain anterior to the oral apparatus that drives feeding flows and a sparser, slower domain that drives swimming.

Neural prediction decorrelation reveals that adversarial robustness substantially improves DNN prediction accuracy across the entire human auditory cortex

Sounds synthesized to decorrelate model predictions across human auditory cortex show an adversarially robust CochResNet50 predicts fMRI responses far better than its standard counterpart, a gap hidden with natural sounds.

Unraveling phenotypic variation in multiscale behavioral dynamics

Comparing coarse-grained dynamics across resolutions, weighted by sampling uncertainty, yields phenotypic spaces linking E. coli run speed to CheB and showing paramecia experience narrows zebrafish variability.

Mapping the Trade-Offs That Shape Mammalian Brain Network Architecture

Across 25,000 generative-model networks scored on 29 properties, 635 human and 224 mammalian connectomes cluster in one narrow niche, trading wiring economy for computational capability and robustness.

Structural Basis of Human Kinetochore-Microtubule Coupling by the Ndc80 and Ska Complexes

Cryo-EM shows human Ndc80 and Ska complexes bound to adjacent microtubule protofilaments, linked by a SKA3 tether that passes Ska's preference for GTP-like lattice and tip tracking to Ndc80.

A surviving beta cell subpopulation enriched in patients with T1D

Regulon-based clustering of islet scRNA-seq finds an IRF1-high, cytokine-driven beta cell state with reduced identity and autoantigen genes, enriched in type 1 diabetes donors.

KAS-CUT&Tag for direct mapping of transcription bubbles

N3-kethoxal labeling of single-stranded DNA within CUT&Tag maps Pol II transcription bubbles; bubble density tracks nascent transcription, shifts with transcription inhibitors and TFIIS loss, and peaks at histone genes.

PTEN Subcellular Localization Dictates Function

Nuclear-excluded PTEN rescued all knockout overgrowth phenotypes in mouse dentate granule neurons; nuclear-restricted PTEN rescued only spine head area and partly soma size, so localization shapes function.

Multi-Trajectory Pseudotime Inference via Permutation Factorizations

Factorizing multiple monotone pseudotimes into one shared donor permutation plus cluster-specific warps (HiP-BIND) yields Alzheimer's donor orderings agreeing better with Braak, ADNC and CERAD staging than single-trajectory B-BIND.

Topological structure in human spatial representation revealed through drawing

When redrawing simple line figures from memory, adults and 5- to 8-year-olds pulled angles toward 90° yet kept T-junctions more often than L-junctions; adults also compressed length ratios.

Degraded myelin is associated with cold hypersensitivity in paclitaxel-induced peripheral neuropathy

In paclitaxel-treated mice, cold hypersensitivity outlasts mechanical hypersensitivity and coincides with reduced myelin gene expression, lower chromatin accessibility at Egr2/Yy1 motifs, and fragmented sciatic nerve myelin.

Patient-Derived Liver Cancer Organoids Reflect Tumor Biology and Their Growth Phenotype Correlates with Clinical Outcomes

Organoids grew from 13 of 27 resected hepatocellular carcinomas. The sequenced lines kept parental mutations, and solid rather than cystic growth marked shorter progression-free survival (HR 13.91).