Cellular transport and secretion

This paper shows that N-terminal tagging of Exo70 disrupts its assembly with the exocyst complex and alters its spatiotemporal behavior at the plasma membrane.

The paper shows that disrupting the actin cytoskeleton leads to the formation of a giant unilocular vacuole, which confers anoikis resistance in cells losing matrix attachment.

This study identifies USP8's role in endosome maturation by mediating Rab5-to-Rab7 switch through recruitment by Rabex5 and promotion of SAND-1/Mon1 recruitment.

The paper identifies a minimal set of presynaptic proteins that regulate different modes of Ca2+-triggered vesicle fusion and short-term plasticity.

The paper identifies two Fam21 fragments interacting with VPS35 and a third interacting with VPS29, revealing distinct binding modes and structural insights into retromer-WASH complex recruitment.

The paper maps the interactome of ARF and ARL proteins using BioID, identifies new localizations, and reveals functional roles of ARL14 in activating PLD1 and cargo trafficking.

The paper challenges the classical cisternal progression model by showing that intra-Golgi transport velocity varies and Golgi residence times differ for different cargos, suggesting a stable compartment model.

This study identifies N-terminal signals in Vin1 and Vps5 that guide the formation of specific endosomal coat complexes in yeast.

The paper explores how liquid-liquid phase separation regulates dense-core vesicle function, specifically the differential release of catecholamines and ATP.

The paper shows that neutralizing late endosomal pH via LLOMe or NH4Cl hyper-activates Rab7 through V-ATPase and RILP, disrupting tubulation and CI-M6PR trafficking.

The paper investigates how point mutations in Arf1 affect its interaction with GAPs, revealing cooperative effects between the N-terminal region and myristate on catalytic activity.

The paper identifies a mechanism by which TBC1D23 recognizes transport vesicles destined for the Golgi through direct binding to a shared cargo motif.

The paper introduces PICT to measure protein distances in living cells, showing exocyst subunit and interacting protein spatial configurations.

This paper identifies design rules for efficient endosomal escape by showing that protein size and thermal stability influence delivery efficiency when conjugated to ZF5.3.

This study shows tomosyns inhibit synaptic depression by binding to VAMP2-containing template complexes and preventing SNAP-25 association, rather than competing with VAMP2 for SNARE complex assembly.

This study investigates the role of the C-terminal half of the SNARE domain of Syntaxin-1A in synaptic vesicle stability and neurotransmitter release regulation.

This study shows that otoferlin's free C-terminal end is essential for synaptic vesicle docking and fusion in inner hair cells, as demonstrated by deafness and reduced exocytosis in a knock-in mouse model.

The paper investigates the role of DRP2s and SH3P2 in plant clathrin-mediated endocytosis, finding that DRP2s are recruited independently of BAR-SH3 proteins.

This study shows that SNX4 limits synaptic vesicle docking and release by affecting active zone structure and vesicle density.

The paper investigates AP-1 function in membrane traffic, showing it retrieves proteins from post-Golgi compartments back to the TGN, similar to COPI's role in the early secretory pathway.

This paper introduces recombinant biosensors with SNAP tags for multiplex and super-resolution imaging of all 8 phosphoinositides in fixed cells and tissues.

This study identifies Sbh1/Sec61β as a specific signal peptide receptor that recognizes and guides substrates into the Sec61 channel through its cytosolic IDR and conserved regions.

This study identifies Exo70's role in herpesvirus secretory vesicle tethering and virion release via the exocyst complex, showing ES2 inhibits herpesvirus infection.

This study uses lattice light sheet microscopy to analyze cell plate development in four dimensions, identifying phase transitions and the role of callose in cytokinesis.

This study determines the cryoEM structure of autoinhibited Sec7 and proposes an active conformation model based on functional and structural analyses.

This study investigates how ALG-2 binds membranes and proteins in a Ca2+-dependent manner, revealing its role in membrane repair and ERES function.

This paper reconstructs the evolution of ESCRT systems in archaea, identifying homologs in Asgardarchaeota and other groups, suggesting an early origin of ESCRT components and their connection to the ubiquitin system.

The paper investigates Drosophila p24 proteins and finds that their interaction with Tango1 is essential for maintaining ER-Golgi interface stability and efficient transport.

The paper shows that weakening of septate junctions in Drosophila epithelial cells triggers changes in adhesive and mechanical properties through ESCRT-III and Retromer pathways.

This study shows that the VPS35 D620N mutation enhances LRRK2 activity and amplifies its response to endolysosomal stress, leading to increased phosphorylation of RAB10 and RAB12.