D. E. Kyle

Extended blood stage sensitivity profiles of Plasmodium vivax to doxycycline and tafenoquine using Plasmodium cynomolgi as a model

The paper uses Plasmodium cynomolgi to determine the delayed antimalarial effects of doxycycline and tafenoquine on P. vivax, finding IC50 values for doxycycline and tafenoquine.

Oviposition status and sugar availability influence measures of transmission potential in malaria-infected mosquitoes.

This study shows that oviposition status and sugar availability affect malaria parasite transmission potential in mosquitoes.

Blood meals from 'dead-end' vertebrate hosts enhance transmission potential of malaria-infected mosquitoes

The paper shows that additional blood meals from bovine and canine hosts can enhance sporozoite migration in malaria-infected mosquitoes, with human and bovine blood showing more consistent reductions in extrinsic incubation period.

Liver stage fate determination in Plasmodium vivax parasites: characterization of schizont growth and hypnozoite fating from patient isolates

This study investigates how P. vivax patient isolates and hepatocyte donor lots influence hypnozoite formation and schizont growth in liver stage cultures.

Single-cell RNA profiling of Plasmodium vivax-infected hepatocytes reveals parasite- and host- specific transcriptomic signatures and therapeutic targets

This study uses single-cell RNA sequencing to analyze P. vivax-infected hepatocytes, identifying transcriptomic differences between schizonts and hypnozoites and potential therapeutic targets.

Direct type I Interferon signaling in hepatocytes control malaria

This study shows that direct type I interferon signaling in infected hepatocytes is necessary and sufficient to control malaria, revealing cell-autonomous immune responses.

Differential growth rates and in vitro drug susceptibility to currently used drugs for multiple isolates of Naegleria fowleri

This study evaluates drug susceptibility and growth rates of 11 Naegleria fowleri isolates, revealing significant variation in responses to 7 of 8 tested drugs and growth rates, emphasizing the need for diverse isolates in drug development.

Alkyne modified purines for assessing activation of Plasmodium vivax hypnozoites and growth of pre-erythrocytic and erythrocytic stages in Plasmodium spp.

This study develops alkyne-modified purines as a DNA synthesis marker to distinguish replicating and non-replicating Plasmodium stages, aiding in understanding dormancy mechanisms.

Altered cleavage of Caspase-1 in hepatocytes limits control of malaria in the liver

This study shows that limited Caspase-1 cleavage in hepatocytes reduces pyroptosis and cytokine maturation, impairing malaria control, and that enhancing ASC expression improves immune response.

EdU incorporation to assess cell proliferation and drug susceptibility for the brain-eating amoeba, Naegleria fowleri

This study validates EdU incorporation as a method to assess cell proliferation and drug susceptibility in Naegleria fowleri, showing its utility in drug screening.

The tubovesicular network in Plasmodium vivax liver-stage hypnozoites and schizonts associates with host aquaporin 3

This study uses high-resolution microscopy to show AQP3 associates with the tubovesicular network in P. vivax hypnozoites and schizonts, revealing dynamic host-parasite interactions during liver-stage infection.

Discovery of anti-amoebic inhibitors from screening the MMV Pandemic Response Box on Balamuthia mandrillaris, Naegleria fowleri and Acanthamoeba castellanii

The paper screens a chemical library to identify anti-amoebic inhibitors effective against three pathogenic amoebae, finding multiple compounds with nanomolar to low micromolar potency.