D. Koo

Optimizing Cell Therapy by Sorting Cells with High Extracellular Vesicle Secretion

The paper develops a method to enrich cells with high extracellular vesicle secretion and shows improved therapeutic outcomes in a mouse model.

STORM imaging buffer with refractive index matched to standard immersion oil

This paper introduces a STORM imaging buffer with refractive index matched to standard immersion oil, improving imaging depth and resolution for thick tissues.

Defining T cell receptor repertoires using nanovial-based affinity and functional screening

This study uses nanovials to screen antigen-specific T cells based on affinity and function, identifying a more specific functional TCR repertoire than standard techniques.

Secretion encoded single-cell sequencing (SEC-seq) uncovers gene expression signatures associated with high VEGF-A secretion in mesenchymal stromal cells

SEC-seq links cell secretions to transcriptomes, revealing VEGF-A secretion heterogeneity and a unique gene signature in MSCs with high secretion.

Sorting Single T cells based on Secreted Cytokines and Surface Markers using Hydrogel Nanovials

This paper develops a method to sort T cells based on cytokine secretion and surface markers using hydrogel nanovials, finding that polyfunctional cells are more common in CD4+ T cells and remain viable after sorting.

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

The paper develops a scalable method to produce hydrogel microparticles with localized surface chemistry for cell interfacing, achieving high throughput and precise shape and functionalization.

Massively parallel encapsulation of single cells with structured microparticles and secretion-based flow sorting

This paper introduces a method to sort single cells based on secreted products using microparticles, enabling high-throughput screening of cell function.