K. Lorenzen

Light-induced Trpin/Metout switching during BLUF domain activation in ATP-bound photoactivatable adenylate cyclase OaPAC

This study investigates the light-induced structural changes in OaPAC's BLUF domain, revealing a Trpin/Metout transition and conformational shifts during ATP activation.

Convolutional neural network approach for the automated identification of in cellulo crystals

This paper uses a convolutional neural network to automatically identify in cellulo crystals in living insect cells from bright field images.

Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography

This paper introduces a mix-and-extrude method for time-resolved protein crystallography using 3D printed microchannels to study ligand-induced reactions in viscous media.

SARS-CoV-2 papain-like protease PLpro in complex with natural compounds reveal allosteric sites for antiviral drug design

This study identifies three natural compounds that inhibit SARS-CoV-2 PLpro by binding to the ISG15/Ub-S2 allosteric site, showing antiviral activity and potential for drug design.

Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX

This paper determines the structure of Tpp49Aa1 using MHz-SFX and investigates its interaction with Cry48Aa1, expanding its known target range to include additional mosquito species.

Inhibition of SARS-CoV-2 main protease by allosteric drug-binding

The paper identifies 37 compounds binding to SARS-CoV-2 main protease through X-ray crystallography, with five non-peptidic compounds showing antiviral activity in cell-based assays.

Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes

This study determines the stoichiometry and topology of nsp7+8 complexes from seven coronaviruses using mass spectrometry and biophysical techniques, revealing three clustering groups with distinct assembly patterns.

Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease

The paper presents the crystal structure of SARS-CoV-2 main protease covalently bound to a metabolite of HEAT, revealing a pro-drug mechanism and potential for non-peptidic inhibitors.