H. Han

Diffusing protein binders to intrinsically disordered proteins

The paper develops RFdiffusion to generate protein binders for IDPs/IDRs, achieving nanomolar affinity and functional applications in amylin and IL2RG.

A MSTNDel273C mutation with FGF5 knockout sheep by CRISPR/Cas9 promotes skeletal muscle myofiber hyperplasia

This paper describes the creation of MSTNDel273C mutation with FGF5 knockout sheep using CRISPR/Cas9, resulting in skeletal muscle myofiber hyperplasia and a 'double-muscle' phenotype.

Non-Invasive Quality Control of Organoid Cultures Using Mesofluidic CSTR Bioreactors and High-Content Imaging

This paper develops a mesofluidic bioreactor platform with high-content imaging for non-invasive quality control of brain organoids, improving their development and reducing cell death.

antiCD49d Ab treatment ameliorates age-associated inflammatory response and mitigates CD8 T cell cytotoxicity after traumatic brain injury

This study shows that anti-CD49d antibody treatment reduces age-related inflammatory responses and CD8 T cell cytotoxicity after traumatic brain injury in mice.

The long-chain fatty acid-CoA ligase FadD1 monitors Pseudomonas aeruginosa quorum sensing as a receptor of cis-2-decenoic acid

This paper identifies FadD1 as a receptor for cis-DA quorum sensing in P. aeruginosa, showing it regulates virulence and physiology by binding cis-DA and modulating gene expression.

Minimized Sample Consumption for Time-Resolved Serial Crystallography Applied to the Redox Cycle of Human NQO1

This paper demonstrates a method to reduce sample consumption in time-resolved serial crystallography by using segmented droplet generation and mix-and-inject approaches for studying NQO1's redox cycle.

Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography

The paper determines the crystal structure of a bacterial photoactivated adenylate cyclase at room temperature using serial femtosecond crystallography and compares it with cryo structures to study structural changes upon light exposure.

Double-stranded RNA induces retinal pigment epithelium cell degeneration and inflammation

This study shows dsRNA triggers RIG-I signaling, leading to inflammation and RPE degeneration in AMD, with implications for pathogenesis.

Cell Type Specific Responses of the Endoplasmic Reticulum Dynamics to Environmental Stress

This study develops a super-resolution imaging and analysis method to study ER dynamics in Arabidopsis under environmental stress, revealing correlations between ER structure parameters and growth features.

Inhibiting Stromal Class I HDACs Curbs Pancreatic Cancer Progression

This study shows that inhibiting stromal Class I HDACs reduces pancreatic cancer progression by targeting desmoplastic and pro-tumorigenic programs in cancer-associated fibroblasts.

Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP

This study demonstrates that lipid nanoparticle-delivered thermostable Cas9 RNPs achieve high-efficiency genome editing in mouse liver and lung tissues.

Identification and functional analysis of eRNA markers for hepatocellular carcinoma based on high-throughput data

This study identifies three eRNA biomarkers for hepatocellular carcinoma (HCC) and shows their involvement in cancer progression, prognosis, and potential as therapeutic targets.

Light-induced Trpin/Metout switching during BLUF domain activation in ATP-bound photoactivatable adenylate cyclase OaPAC

This study investigates the light-induced structural changes in OaPAC's BLUF domain, revealing a Trpin/Metout transition and conformational shifts during ATP activation.

A Stapled Peptide Inhibitor of METTL3-METTL14 for Cancer Therapy

This paper develops a stapled peptide inhibitor of METTL3-METTL14 that suppresses tumor growth by inhibiting m6A methylation and promoting METTL3 degradation.

Heterologous expression, purification and structural features of native Dictyostelium discoideum dye-decolorizing peroxidase bound to a natively incorporated heme

This study expresses and purifies a functional DdDyP peroxidase with native heme incorporation, determines its structure, and reveals key catalytic site features.

Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency

This study improves a DNA polymerase's ability to incorporate modified nucleotides through combinatorial mutagenesis, resulting in a 20-fold efficiency gain.

Sanglifehrin A mitigates multi-organ fibrosis in vivo by inducing secretion of the collagen chaperone cyclophilin B

This study identifies cyclophilin B as the target of sanglifehrin A, showing it reduces collagen synthesis in fibrotic tissues by inducing cyclophilin B secretion.

Suggested role of NosZ in preventing N2O inhibition of dissimilatory nitrite reduction to ammonium

This study investigates how N2O affects DNRA in Bacillus sp. DNRA2, finding that N2O delays DNRA transition unless removed by NosZ, suggesting nosZ retention helps prevent N2O inhibition.

Transcription regulation of SARS-CoV-2 receptor ACE2 by Sp1: a potential therapeutic target

This study identifies Sp1 as a regulator of ACE2 expression in response to SARS-CoV-2 infection and shows that targeting Sp1 reduces viral replication and tissue injury in Syrian hamsters.

Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography

This paper introduces a mix-and-extrude method for time-resolved protein crystallography using 3D printed microchannels to study ligand-induced reactions in viscous media.

SARS-CoV-2 papain-like protease PLpro in complex with natural compounds reveal allosteric sites for antiviral drug design

This study identifies three natural compounds that inhibit SARS-CoV-2 PLpro by binding to the ISG15/Ub-S2 allosteric site, showing antiviral activity and potential for drug design.

A Genome-scale Screen Reveals ANTXR1, Heparan Sulfate and Neu5Gc as Host Factors mediating Seneca Valley virus Entry into Porcine Cells

This study identifies ANTXR1, heparan sulfate, and Neu5Gc as host factors required for SVV entry into porcine cells using CRISPR screening.

Vomocytosis of Cryptococcus neoformans cells from murine, bone marrow-derived dendritic cells

This study shows that Cryptococcus neoformans can be expelled from murine bone marrow-derived dendritic cells via vomocytosis, similar to macrophages, and is inhibited by phagosome and actin-modifying drugs.

Domain fusion TLR2-4 enhances the autophagy-dependent clearance of Staphylococcus aureus in the genetic engineering goat generated by CRISPR/Cas9

This study generates genetically engineered goats expressing a domain fusion TLR2-4 receptor to enhance autophagy-dependent clearance of Staphylococcus aureus in macrophages.

Characterization of the minimal residual disease state reveals distinct evolutionary trajectories of human glioblastoma

This study characterizes the minimal residual disease state in glioblastoma, revealing distinct evolutionary trajectories and immunomodulatory signatures associated with recurrence.

Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling

The paper uses CETSA MS to identify novel crosstalk between brassinosteroid and auxin signaling in Arabidopsis by analyzing the thermal and phospho-proteomes of the GSK3 inhibitor bikinin.

Eicosanoids in the pancreatic tumor microenvironment: a multicellular, multifaceted progression

The paper identifies key eicosanoid changes in pancreatic tumorigenesis and the cell types responsible for their synthesis using mouse models and patient samples.

Endogenous RNA editing of a nuclear gene BOSS triggers flowering in tomato

The paper identifies RNA editing of the BOSS gene in tomato that regulates flowering by producing functionally distinct transcripts.

Transcriptional Inactivation of TP53 and the BMP Pathway Mediates Therapy-induced Dedifferentiation and Metastasis in Prostate Cancer

The paper identifies a novel subtype of therapy-induced prostate cancer cells with hybrid E/M and stem-like traits, showing their dependency on HER2/3 signaling and resistance to AR inhibition.

Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis

The paper identifies a rapid auxin-mediated phosphorylation of Myosin XI and MadB2 that regulates PIN transporter trafficking and polarity in Arabidopsis, revealing a non-canonical auxin response mechanism.