P. PADDISON

Classifying cell cycle states and a quiescent-like G0 state using single-cell transcriptomics

The paper introduces ccAFv2, a high-resolution cell cycle classifier for scRNA-seq data, which outperforms existing methods and classifies six cell cycle states and a quiescent-like G0 state.

Functional genomic analysis of adult and pediatric brain tumor isolates

This study identifies shared and unique gene dependencies between adult and pediatric brain tumor isolates using CRISPR screens and machine learning models.

FBXO42 activity is required to prevent mitotic arrest, spindle assembly checkpoint activation, and lethality in glioblastoma and other cancers

This study identifies FBXO42 as essential for preventing mitotic arrest and cell death in glioblastoma and other cancers by suppressing chromosome-microtubule dynamics disruption.

Gene knock-outs in human CD34+ hematopoietic stem and progenitor cells and in the human immune system of mice

The paper develops a method to efficiently edit human CD34+ HSPCs before transplantation, enabling gene knock-outs in the human immune system of mice with high efficiency and without affecting engraftment potential.

KAT5 regulates neurodevelopmental states associated with G0-like populations in glioblastoma

This study identifies KAT5 as a regulator of G0-like states in glioblastoma, showing its role in suppressing non-dividing subpopulations and associating with worse patient outcomes.

Functional dissection of human mitotic proteins using CRISPR-Cas9 tiling screens

The paper uses CRISPR-Cas9 tiling screens to identify functional domains in human mitotic proteins, revealing new regions essential for cell proliferation and chromosome segregation.

A simple and highly efficient method for multi-allelic CRISPR-Cas9 editing in primary cell cultures

This paper introduces an optimized CRISPR-Cas9 method for efficient gene knockout and genomic deletion in primary cells, achieving >90% indel formation in 3 days.

Neural G0: a quiescent-like state found in neuroepithelial-derived cells and glioma

The paper identifies a quiescent-like state, Neural G0, in human neural stem cells and gliomas using scRNA-seq, linking it to tumor aggressiveness and survival.

N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis

The paper identifies m6A mRNA methylation as a regulator of erythropoiesis by promoting the translation of key genes involved in erythroid lineage specification.