P. Tinnefeld

Cooperative dynamics of DNA grafted magnetic nanoparticles optimize magnetic biosensing and coupling to DNA origami

This study shows that DNA grafting density on magnetic nanoparticles affects their dynamics and biosensing performance, with optimal sensitivity at intermediate densities.

Single-Molecule FRET at 10 MHz Count Rates

This paper demonstrates a method using DNA nanoantennas to increase photon count rates in smFRET, enabling observation of fast biomolecular dynamics with improved temporal resolution.

Real-Time Structural Biology of DNA and DNA-Protein Complexes on an Optical Microscope

This study develops a method to study DNA and DNA-protein interactions with high spatial and temporal resolution using graphene and energy transfer.

Engineering Modular and Tunable Single Molecule Sensors by Decoupling Sensing from Signal Output

This paper introduces modular and tunable single-molecule sensors using DNA origami scaffolds to detect biomolecules with adjustable response windows and specificity.

DNA Origami Vesicle Sensors with Triggered Cargo Transfer

The paper describes a DNA origami-based biosensor that detects lipid vesicles through smFRET and enables targeted cargo transfer to vesicles.

Engineering an artificial catch bond using mechanical anisotropy

The paper demonstrates how modifying anchor geometry can generate catch bonding behavior in the DocG:CohE adhesion complex, showing mechanical anisotropy enables engineered catch bonds.

Super-Resolved FRET and Co-Tracking in pMINFLUX

This paper introduces two methods in pMINFLUX to enable nanometer-precise co-localized tracking of fluorophores, bridging the resolution gap between smFRET and co-tracking.

Deep-Learning Assisted, Single-molecule Imaging analysis (Deep-LASI) of multi-color DNA Origami structures

Deep-LASI is a deep learning tool for rapid analysis of single-molecule imaging data, demonstrating accuracy and speed in FRET experiments and multi-color data.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

DNA Origami Voltage Sensors for Transmembrane Potentials with Single-Molecule Sensitivity

The paper develops DNA origami-based voltage sensors with single-molecule sensitivity, showing a 5% FRET change for 100 mV transmembrane potential shifts.

Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope

This paper introduces NACHOS, a DNA origami-based nanoantenna system that enables single-molecule detection using a smartphone microscope, reducing costs and complexity.

High Force Catch Bond Mechanism of Bacterial Adhesion in the Human Gut

The paper identifies a catch bond mechanism in a bacterial adhesion complex that resists high shear forces in the human gut.

Cohesin-dockerin code in cellulosomal dual binding modes and its allosteric regulation by proline isomerization

The paper investigates cohesin-dockerin interactions in cellulosomes, revealing two binding modes and an allosteric regulation mechanism via proline isomerization.

DNA origami-based single-molecule force spectroscopy unravels the molecular basis of RNA Polymerase III pre-initiation complex stability

The paper uses DNA origami force clamps to study how force affects initiation complex stability in RNA polymerase II and III systems, revealing Bdp1's critical role in RNAP III stability.