J. Piehler

Long-term single molecule localization microscopy uncovers dynamic co-assembly of Lrp6 and Ror2 into Wnt-signalosomes

This study uses single-molecule tracking to show that Lrp6 and Ror2 co-assemble into hybrid Wnt signalosomes dynamically.

Tuning of granulopoietic signaling by de novo designed agonists

This paper designs and characterizes agonists that tune G-CSFR activity to bias granulopoiesis over proliferation, showing improved stability and selectivity compared to native G-CSF.

Structure and regulation of GSDMD pores at the plasma membrane of pyroptotic cells

The paper visualizes GSDMD nanostructures at the plasma membrane of pyroptotic cells and identifies PI(3,4,5)P3 as a regulator of pore formation.

Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane

The paper combines TIRF and structured illumination microscopy to enable quantitative single-molecule analysis and track receptor dynamics in the plasma membrane.

Specificity and promiscuity of JAK recruitment regulates pleiotropy of cytokine-receptor signaling

The paper investigates JAK recruitment specificity and promiscuity in cytokine-receptor signaling, finding that JAKs can interchangeably bind to receptors, leading to cell type-specific signaling pleiotropy.

Differential functional coupling in Gp130-JAK complexes expands the plasticity of the interleukin-6 signaling axis

The paper investigates how different JAKs in Gp130 signaling complexes lead to distinct STAT activation profiles and signaling outcomes.

Biofunctional nanodot arrays in living cells uncover synergistic co-condensation of Wnt signalodroplets

The paper develops biofunctional nanodot arrays to study Wnt signalodroplets in live cells, revealing synergistic co-condensation through liquid-liquid phase separation.

Plasma membrane topography governs the three-dimensional dynamic localization of IgM B cell receptor clusters

This study uses lattice light sheet microscopy to show that plasma membrane topography influences the dynamic localization of IgM B cell receptor clusters in Ramos B cells.

Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway

The paper shows pathogenic SMS2 variants disrupt lipid landscapes in the secretory pathway by trapping sphingomyelin in the ER and altering cholesterol and glycerophospholipid distributions.

Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain Dimers

This study investigates the formation of non-covalent MHC-I heavy chain dimers after dissociation of β2m and peptide, revealing a dimerization interface mediated by the 3 domain.

Tuning TPO-R signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia

This study develops diabody ligands that modulate TPO-R signaling, showing partial agonists preserve HSCs and inhibit ET oncogenesis.

TIN2 facilitates TRF1-mediated trans- and cis-interactions on physiologically relevant long telomeric DNA

This study shows TIN2 promotes TRF1-mediated DNA-DNA bridging on telomeres, stabilizing interactions that depend on telomere length and sequence.

Parallelized manipulation of adherent living cells by magnetic nanoparticles-mediated forces

This study demonstrates that magnetic nanoparticle-mediated forces from micro-magnetic pillars can direct cell migration and neurite outgrowth in adherent cells.

Engineered IL-10 variants elicit potent immuno-modulatory activities at therapeutic low ligand doses

This study engineered IL-10 variants with enhanced receptor affinity, showing improved bioactivity and gene expression at lower doses in human monocytes and CD8 T cells.

Kinetics of cytokine receptor trafficking determine signaling and functional selectivity

The paper investigates how cytokine receptor binding kinetics affect signaling outcomes by engineering IL-6 variants with different affinities to gp130.