D. Nicastro

The ciliary MBO2 complex targets assembly of inner arm dynein b and reveals additional doublet microtubule asymmetries

The paper identifies the MBO2 complex as a regulator of inner arm dynein b assembly in cilia and reveals its structural organization on doublet microtubules.

Cryo-electron tomography of eel sperm flagella reveals a molecular "minimum system" for motile cilia

This study uses cryo-electron tomography to analyze the structure of eel sperm flagella, revealing a minimal system with retained remnants of regulatory complexes and unique structural features.

Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery

This study uses cryo-electron tomography to show that the apical complex in apicomplexan parasites moves as a rigid body, with the conoid structure remaining unchanged during protrusion and retraction.

Triglyceride lipolysis driven by glucose restriction triggers liquid-crystalline phase transitions and proteome remodeling of lipid droplets

The paper shows glucose restriction induces liquid-crystalline phase transitions in lipid droplets by promoting TG lipolysis, which alters lipid composition and remodels the proteome.

Three-dimensional cilia structures from animals' closest unicellular relatives, the Choanoflagellates

This study uses cryo-electron tomography to analyze cilia structures in Choanoflagellates, revealing features more similar to metazoan cilia than to those of other unicellular organisms.

Liquid-crystalline lipid phase transitions in lipid droplets selectively remodel the LD proteome

The paper shows glucose restriction induces liquid-crystalline lipid phase transitions in lipid droplets, which selectively remodel the LD proteome and affect ER-LD transport.

3D structure and in situ arrangements of CatSper channel in the sperm flagellum

This study uses cryo-electron tomography to reveal the 3D structure and arrangement of the CatSper channel complex in mammalian sperm, identifying new associated proteins and structural features critical for flagellar function.

Structural organization of the C1b projection within the ciliary central apparatus

The paper identifies and characterizes the subunit organization of the C1b projection in the central apparatus of cilia, revealing its role in regulating dynein activity and ciliary beating.

Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus

The paper identifies and localizes subunits of the ciliary central apparatus using cryo-electron tomography and genetic approaches in Chlamydomonas.

FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia

This study identifies FAP57/WDR65 as a docking factor that targets specific inner dynein arms to the 96 nm axonemal repeat and connects to regulatory hubs in cilia.

Scaffold subunits support associated subunit assembly in the Chlamydomonas ciliary nexin-dynein regulatory complex

This study uses cryo-electron tomography to determine the structure of the N-DRC in Chlamydomonas flagella and identifies key subunit interactions and assembly requirements.

Complexity and ultrastructure of infectious extracellular vesicles from cells infected by nonenveloped virus

This study identifies the complex composition and structure of infectious extracellular vesicles from non-enveloped virus-infected cells, revealing their role in viral transmission.

Tetrahymena RIB72A and RIB72B are Microtubule Inner Proteins in the ciliary doublet microtubules

The paper identifies RIB72A and RIB72B as Microtubule Inner Proteins in Tetrahymena ciliary doublet microtubules and shows their role in ciliary structure and motility.

Targeted dynein inhibition generates flagellar beating

The paper uses cryo-electron tomography to visualize dynein conformations in sea urchin sperm flagella, revealing a mechanism where dynein inhibition generates flagellar beating through asymmetric force distribution.