Author

E. Rhoades

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biophysics· 6 Biochemistry· 2 Bioengineering· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 10 Research Focus:cell signaling & trafficking· 7 Research Focus:protein folding & dynamics· 4 Organism:human· 3 Assay:protein structure· 3 Assay:proteomics· 3 Assay:fluorescence / live-cell microscopy· 2 Research Focus:cell type annotation· 1

Frequent Co-Authors

E. J. Petersson· 2 shared J. Ramirez· 2 shared K. McKibben· 2 shared M. Birol· 2 shared A. A. Pal· 1 shared A. D. Miranker· 1 shared A. Idris· 1 shared B. Pan· 1 shared

Activity by Year

2017-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 7 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersBiochemistry

E. J. Petersson

Mutual co-authors in cluster

Author profile →

10 papers

Recent·Most cited

The C-terminus of alpha-Synuclein regulates its dynamic cellular internalization by Neurexin 1 beta

M. Birol, I. I. Dominguez Munoz, E. Rhoades

This study identifies the role of alpha-Synuclein's C-terminus in its internalization via Neurexin 1 beta, showing that truncation beyond residue 90 prevents internalization and that S-N1{beta} interactions may be therapeutically targeted.

bioRxiv · Jul 28, 2023Biophysics

Nanomolar interactions of alpha-synuclein fibrils to tau determined by FCS

J. Ramirez, E. A. E. Brackhahn, E. J. Petersson, E. Rhoades

The paper investigates interactions between tau and alpha-synuclein using FCS, finding stronger binding with fibrillar alpha-synuclein than monomers, without affecting tau aggregation.

bioRxiv · Apr 13, 2023 · PreprintBiochemistry

Semi-synthetic CoA-alpha-Synuclein Constructs Trap N-terminal Acetyltransferase NatB for Binding Mechanism Studies

B. Pan, S. Gardner, K. Schultz, R. Perez, S. Deng, M. Shimogawa, K. Sato, E. Rhoades, R. Marmorstein, E. J. Petersson

This study creates a semi-synthetic inhibitor of NatB using coenzyme A and full-length alpha-synuclein, revealing structural and conformational changes upon binding.

bioRxiv · Apr 3, 2023Biochemistry

Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter.

T. R. Mumford, D. Rae, E. Brackhahn, A. Idris, D. Gonzalez-Martinez, A. A. Pal, M. C. Chung, J. Guan, E. Rhoades, L. J. Bugaj

This paper introduces CluMPS, a fluorescent reporter that detects and amplifies submicroscopic protein clusters, enabling visualization of small oligomers and endogenous protein assemblies.

bioRxiv · Jul 13, 2022Bioengineering

Tau avoids the GTP cap at growing microtubule plus ends

B. T. Castle, K. M. McKibben, E. Rhoades, D. J. Odde

This paper shows that tau preferentially binds GDP-tubulin over GTP-tubulin, causing it to lag behind microtubule plus-ends and suppress shortening rather than promote growth.

iScience · Dec 1, 2020Peer-reviewedCell Biology

Structural characterization of tau in fuzzy tau:tubulin complexes

H. Y. J. Fung, K. McKibben, J. Ramirez, K. Gupta, E. Rhoades

This study characterizes the structure of tau in fuzzy tau:tubulin complexes using fluorophore probes and compares it to cryo-EM models of tau:MT complexes.

bioRxiv · Nov 21, 2019Biophysics

Regulation of tau’s proline rich region by its N-terminal domain

K. McKibben, E. Rhoades

This study identifies tau's proline rich region as an autonomous tubulin-binding domain that enhances polymerization, while the N-terminal domain negatively regulates these interactions.

bioRxiv · May 13, 2019Biophysics

Polyphosphate initiates tau aggregation through intra- and intermolecular scaffolding

S. P. Wickramasinghe, J. Lempart, H. E. Merens, J. Murphy, U. Jakob, E. Rhoades

This study shows polyphosphate initiates tau aggregation by altering its conformation and enabling noncovalent cross-linking.

bioRxiv · Mar 25, 2019Biophysics

Identification of N-linked glycans as specific mediators of neuronal uptake of acetylated α-Synuclein

M. Birol, S. P. Wojcik, A. D. Miranker, E. Rhoades

This study identifies N-linked glycans as mediators of neuronal uptake of acetylated α-Synuclein, showing that they facilitate internalization while unmodified α-Synuclein does not interact with them.

bioRxiv · Dec 9, 2018Biophysics

Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA and actin filaments

S. Espinoza Sanchez, L. A. Metskas, S. Z. Chou, E. Rhoades, T. D. Pollard

The paper investigates how ATP, WASp-VCA, and actin filaments induce conformational changes in the Arp2/3 complex using FRET and electron microscopy.

bioRxiv · Jul 16, 2017Biophysics