K. Gupta

Assembling a true "Olympic Gel" from >16,000 combinatorial DNA rings

This paper creates a true Olympic gel from over 16,000 DNA rings, showing unique mechanical properties and swelling behavior due to entanglements.

Genome-Wide Identification of G-Quadruplex forming regions in Arabidopsis: Unraveling the Role of Pif like Helicase (AtPLH1) in Gene Regulation and Stress Response

This study identifies G-quadruplex regions in Arabidopsis and shows AtPLH1 regulates gene expression related to stress response and transcription.

A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs

This paper introduces a platform for high-throughput extraction of membrane proteins into native nanodiscs while preserving their local membrane context and provides a database for optimized extraction conditions.

Isolation and Lipidomic Profiling of Neuronal Lipid Droplets: Unveiling the Lipid Landscape of Neurodegenerative Disorders

This paper describes a protocol for isolating and lipidomic profiling of neuronal lipid droplets, revealing their unique lipid composition and potential role in neurodegenerative disorders.

Microcystin-RR is a biliary toxin selective for neonatal cholangiocytes

The paper identifies microcystin-RR as a biliary toxin that selectively damages neonatal cholangiocytes through redox stress mechanisms.

A Gallium-68-Labeled Peptide Radiotracer For CD38-Targeted Imaging In Multiple Myeloma With PET

This paper develops a gallium-68-labeled peptide radiotracer, [68Ga]Ga-AJ206, for non-invasive CD38 imaging in multiple myeloma using PET, showing high specificity and utility in monitoring therapy response.

Antibodies generated in vitro and in vivo elucidate design of a thermostable ADDomer COVID-19 nasal nanoparticle vaccine

This study develops a thermostable ADDomer-based nanoparticle vaccine that neutralizes SARS-CoV-2 and cross-reacts with VOCs, demonstrating its potential for nasal administration.

Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer

This paper develops a platform for analyzing integral membrane proteins and lipids in intact membranes using native mass spectrometry, showing how lipid binding and membrane composition affect protein function.

Human Vascularized Bile Duct-on-a Chip: A multi-cellular micro-physiological system for studying Primary Sclerosing Cholangitis

This paper develops a vascularized bile duct-on-a-chip model to study PSC, showing PSC cholangiocytes exhibit increased inflammation under IL-17A stimulation.

Biliatresone treatment of pregnant mice causes changes in bile metabolism and liver inflammation in their offspring

This study shows that prenatal exposure to low-dose biliatresone in mice leads to altered bile metabolism and liver inflammation in offspring without histological damage.

Determination of oligomeric organization of membrane proteins from native membranes at nanoscale-spatial and single-molecule resolution

This paper introduces a single-molecule imaging technique to measure membrane protein oligomerization in native membranes at high spatial resolution.

Injury and a program of fetal wound healing in the fetal and neonatal extrahepatic bile duct

This study investigates the fetal response to injury in extrahepatic bile ducts (EHBDs) and identifies a fetal wound healing program characterized by high molecular weight HA, which may lead to obstruction in biliary atresia.

Low-intensity transcranial focused ultrasound suppresses pain by modulating pain processing brain circuits

This paper demonstrates that low-intensity transcranial focused ultrasound can suppress pain by modulating pain processing brain circuits in mouse models.

An evolutionarily conserved olfactory receptor is required for sex differences in blood pressure

This study identifies Olfr558/OR51E1 as an evolutionarily conserved receptor mediating sex differences in blood pressure through effects on renin, vascular reactivity, and arterial stiffness.

A transcriptional response to replication stress selectively expands a subset of BRCA2-mutant mammary epithelial cells

The paper shows that BRCA2-mutant mammary epithelial cells with low hormone receptor expression expand under replication stress through a transcriptional response involving Tspan8 and Thrsp.

Dynamic matrices with DNA-encoded viscoelasticity for advanced cell and organoid culture

This paper introduces DyNAtrix, a DNA-encoded hydrogel that allows precise control over viscoelastic properties for 3D cell and organoid cultures.

ATG9 vesicles comprise the seed membrane of mammalian autophagosomes

This study identifies ATG9 vesicles as the seed membrane for mammalian autophagosomes, showing they integrate proteins like LC3-II and are diluted as autophagosomes expand.

Mitochondria morphology governs ATP production rate

This study presents a thermodynamically consistent model of mitochondrial ATP production, showing that ATP production, not export, is the limiting step and linking cytosolic ATP rates to mitochondrial morphology.

Mitoguardin-2 is a lipid transporter and its lipid transfer ability is required for function

This study identifies mitoguardin-2 as a lipid transporter that facilitates lipid transfer between mitochondria and lipid droplets, essential for mitochondrial and lipid droplet function.

Structure of a HIV-1 IN-Allosteric Inhibitor Complex at 2.93 Angstrom Resolution: Routes to Inhibitor Optimization

The paper reports the X-ray structure of an HIV-1 IN-ALLINI complex, identifying pi-mediated interactions that guide inhibitor optimization.

The Free Fatty Acid-Binding Pocket is a Conserved Hallmark in Pathogenic β-Coronavirus Spike Proteins from SARS-CoV to Omicron

The paper identifies a conserved free fatty acid-binding pocket in pathogenic β-coronavirus spike proteins that suppresses viral infection and replication.

Bifidobacterium longum attenuates ovariectomy-induced bone loss via modulating the Immunoporotic Breg-Treg-Th17 cell axis

This study shows that Bifidobacterium longum reduces bone loss in ovariectomy-induced osteoporosis by modulating the Breg-Treg-Th17 immune axis.

Sequential single cell transcriptional and protein marker profiling reveals TIGIT as a marker of CD19 CAR-T cell dysfunction in patients with non-Hodgkin lymphoma

The paper identifies TIGIT as a marker of CD19 CAR-T cell dysfunction in NHL patients through single-cell transcriptional and protein profiling.

Cryptic pathogen-sugar interactions revealed by universal saturation transfer analysis

This paper introduces uSTA, an NMR method to analyze protein-ligand interactions, revealing SARS-CoV-2 spike binds sialoside sugars via the NTD and showing variants-of-concern lose this binding.

Dichotomous regulation of lysosomes by MYC and TFEB controls hematopoietic stem cell fate

This study shows that lysosomes are regulated by TFEB and MYC to balance catabolic and anabolic processes in hematopoietic stem cells, influencing their fate and activation.

Longitudinal preclinical imaging characterization of drug delivery potential after radiotherapy in the healthy and leukemic bone marrow vascular microenvironment

This study uses longitudinal multiphoton imaging to assess how radiotherapy affects drug delivery in the bone marrow vasculature of healthy and leukemic mice.

ALG-097111, a potent and selective SARS-CoV-2 3-chymotrypsin-like cysteine protease inhibitor exhibits in vivo efficacy in a Syrian Hamster model

This study evaluates ALG-097111, a selective SARS-CoV-2 3CLpro inhibitor, showing potent in vitro activity and significant in vivo antiviral effects in Syrian hamsters.

The SARS-CoV-2 Spike protein disrupts human cardiac pericytes function through CD147-receptor-mediated signalling: a potential non-infective mechanism of COVID-19 microvascular disease

This paper shows that SARS-CoV-2 Spike protein causes cardiac pericyte dysfunction through CD147-mediated signaling, without infecting the cells.

Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer

This study identifies a structural motif in the SMN YG box essential for higher-order oligomerization, which is critical for viability and linked to SMA in animal models.

Regulation of gene transcription by thyroid hormone receptor β agonists in clinical development for the treatment of non-alcoholic steatohepatitis (NASH)

This study compares the efficacy of three THRβ agonists in modulating gene expression related to cholesterol and fatty acid metabolism in human and rat models.