J. Kosinski

Nuclear pores as conduits for fluid flow during osmotic stress

This study shows that Dictyostelium discoideum uses nuclear pores as conduits for fluid flow during osmotic stress, distinct from passive diffusion and active transport.

What shapes template matching performance in cryogenic electron tomography in situ

The paper evaluates template matching performance in cryo-ET by analyzing how template size, shape, and angular sampling affect ribosome detection in a ground truth dataset.

An RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment

This study identifies RNA's role in disassembling NP trimers and forming RNA-bound assemblies, and shows Z protein interaction with NP is pH-dependent.

The distance between the plasma membrane and the actomyosin cortex acts as a nanogate to control cell surface mechanics

The paper shows that the distance between the plasma membrane and actomyosin cortex, controlled by MCA proteins, acts as a nanogate regulating actin nucleation and cell surface mechanics.

Form follows function: Variable microtubule architecture in the malaria parasite

The paper uses electron cryo-tomography to study microtubule architecture in malaria parasites across their life cycle, revealing structural diversity not seen in other organisms.

Structure of the mycobacterial ESX-5 Type VII Secretion System hexameric pore complex

The paper presents a high-resolution cryo-EM structure of the ESX-5 Type VII secretion system hexameric pore complex, revealing its assembly and potential gating mechanisms.

Structural role of essential light chains in the apicomplexan glideosome

This study determines the structure of trimeric glideosome subcomplexes in Toxoplasma gondii, revealing conserved interactions and the role of essential light chains in stabilizing myosin A.

In cell architecture of the nuclear pore complex and snapshots of its turnover

The paper uses structural biology and yeast genetics to study NPC architecture and turnover in yeast cells, revealing new insights into mRNA export and autophagy mechanisms.

Nuclear pores constrict upon energy depletion

This study shows that nuclear pore complexes constrict when cells experience energy depletion, linking cellular energy status to NPC conformation.

Structure of the TFIIIC subcomplex {tau} A provides insights into RNA polymerase III pre-initiation complex formation

The paper reports the structure of the S. cerevisiae TFIIIC subcomplex {tau}A and shows how it recruits TFIIIB and positions it upstream of the transcription start site.

In situ architecture of the algal nuclear pore complex

This study reports the in situ architecture of the algal nuclear pore complex in Chlamydomonas reinhardtii and compares it to the human NPC, revealing structural variations between species.