J. Mahamid

Subtomogram averaging of helical filaments in cryo-electron tomography

The paper describes a protocol for subtomogram averaging to generate high-resolution density maps of helical filaments using cryo-electron tomography, applied to mumps virus nucleocapsids and in-cell forms.

An architectural role of oskar mRNA in granule assembly

This study investigates how oskar mRNA's kissing-loop interaction contributes to RNP granule assembly in Drosophila oocytes, showing its role in condensate formation and posterior RNA localization.

Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells

This study uses cryo-electron tomography to show how chloramphenicol affects ribosome structure and translation in Mycoplasma pneumoniae, revealing context-dependent inhibition mechanisms.

The distance between the plasma membrane and the actomyosin cortex acts as a nanogate to control cell surface mechanics

The paper shows that the distance between the plasma membrane and actomyosin cortex, controlled by MCA proteins, acts as a nanogate regulating actin nucleation and cell surface mechanics.

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

This paper introduces genetically encoded multimeric tags for localizing intracellular proteins in cryo-EM, demonstrating their use in human cells with drug-controlled labeling.

Cryo-electron tomography suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules

The paper uses cryo-ET to identify lumenal particles in neuronal microtubules, suggesting some are tubulin chaperones involved in maintaining microtubule structure during neuronal differentiation.

Visualizing translation dynamics at atomic detail inside a bacterial cell

This study uses cryo-electron tomography to visualize translation dynamics in Mycoplasma pneumoniae, revealing ribosome states and showing how antibiotics affect translation.

Determinants shaping the nanoscale architecture of the mouse rod outer segment

This study uses cryo-ET to reveal molecular connectors and supramolecular assemblies in mouse rod outer segments, explaining their structural organization and linking them to genetic diseases.

Molecular mechanisms of stress-induced reactivation in mumps virus condensates

The paper investigates how cellular stress triggers reactivation of persistent mumps virus infection through molecular and structural changes in viral condensates.

Light and electron microscopy continuum-resolution imaging of 3D cell cultures

The paper presents a multi-scale imaging approach for 3D cell cultures that integrates light and electron microscopy to analyze subcellular structures in organoids.

A Modular Platform for Streamlining Automated Cryo-FIB Workflows

The paper introduces SerialFIB, an open-source software tool for automating cryo-FIB workflows, enabling customizable and efficient lamella preparation for cryo-electron tomography.

Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM

This paper introduces a GPU-accelerated 2D template matching method in cisTEM for identifying macromolecular assemblies in cells, demonstrating its utility for in situ proteomics and structure determination.

Liquid-to-solid phase transition of oskar RNP granules is essential for their function in the Drosophila germline

This study shows that oskar RNP granules in Drosophila oocytes undergo a liquid-to-solid phase transition, which is essential for their function in germline formation.

Fluorescence-based 3D targeting of FIB-SEM acquisition of small volumes in large samples

The paper presents a method to precisely target FIB-SEM acquisition of fluorescently labeled cells in large samples using fluorescence preservation and confocal imaging.

In cell architecture of the nuclear pore complex and snapshots of its turnover

The paper uses structural biology and yeast genetics to study NPC architecture and turnover in yeast cells, revealing new insights into mRNA export and autophagy mechanisms.

Nuclear pores constrict upon energy depletion

This study shows that nuclear pore complexes constrict when cells experience energy depletion, linking cellular energy status to NPC conformation.

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.7 A inside cells

The paper introduces a software tool, M, for multi-particle cryo-EM refinement that achieves 3.7 Å resolution of a ribosome-antibiotic complex inside bacterial cells.

Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies

This paper introduces a method to tailor cryo-electron microscopy grids using photo-micropatterning to improve cell positioning and enable in-cell structural studies.