A. Au

TSC22D, WNK and NRBP gene families exhibit functional buffering and evolved with Metazoa for macromolecular crowd sensing

This study identifies functional relationships between TSC22D, WNK, and NRBP gene families in macromolecular crowding sensing and cell volume regulation across metazoans.

A Practical Approach for Optimizing Off-axis Telecentric Digital Holographic Microscope Design

This paper introduces methodologies to optimize the design and alignment of telecentric digital holographic microscopes by determining the optimal +1 order position, verified using living HeLa cells.

Monitoring Non-Specific Adsorption at Solid-Liquid Interfaces by Supercritical Angle Fluorescence Microscopy

This paper extends supercritical angle fluorescence microscopy to monitor dynamic surface phenomena by adding hardware and software, demonstrating its use in tracking non-specific adsorption of proteins and surfactants.

Targeting Apollo-NADP+ to image NADPH generation in pancreatic beta-cell organelles

The paper develops organelle-targeted Apollo-NADP+ sensors to study glucose- and glutamine-stimulated NADPH production in pancreatic beta-cell organelles, revealing mitochondria's key role in NADPH generation.

Nementin is a Nematode-Selective Small Molecule Agonist of Neurotransmitter Release

A C. elegans-based screening pipeline identifies a selective nematicide that also potentiates acetylcholinesterase inhibitors.

Islet-on-a-chip device reveals first phase glucose-stimulated respiration is substrate limited by glycolysis independent of Ca2+ activity.

The paper develops an islet-on-a-chip device to measure glucose-stimulated respiration and Ca2+ activity in individual islets, showing OxPhos is substrate-limited by NADH rather than ADP and independent of Ca2+.

Design of a Versatile Microfluidic Device for Imaging Precision-Cut-Tissue Slices

This paper designs a microfluidic device for 3D fluorescence imaging of precision-cut-tissue slices, enabling controlled media exchange and high-resolution imaging with confocal and SPIM.

A simple, inexpensive and multi scale 3D fluorescent test sample for optical sectioning microscopies

This paper introduces a simple, inexpensive 3D fluorescent test sample made from tissue paper labeled with a highlighter pen for quality control and comparison across optical sectioning microscopies.

Shake-it-off: A simple ultrasonic cryo-EM specimen preparation device

The paper introduces a simple ultrasonic cryo-EM specimen preparation device that improves sample transfer efficiency and reduces air-water interface interactions.