J. L. Rubinstein

Structure of yeast RAVE bound to a partial V1 complex

The paper determines the structure of yeast RAVE bound to a partial V1 complex using cryo-EM, revealing its role in facilitating V1-VO reassociation.

Vesicle Picker: A tool for efficient identification of membrane protein complexes in vesicles

Vesicle Picker automatically identifies vesicles and selects particle locations in cryo-EM micrographs, improving efficiency for membrane protein complex analysis.

High resolution cryo-EM of V-ATPase in native synaptic vesicles

The paper determines the high-resolution structure of V-ATPase in native synaptic vesicles using cryo-EM, revealing interactions with cholesterol and synaptophysin.

Structure and dynamics of a pentameric KCTD5/Cullin3/Gβγ E3 ubiquitin ligase complex

The paper presents the cryo-EM structure of a pentameric KCTD5/CUL3/Gβγ E3 ubiquitin ligase complex, revealing its dynamic architecture and mechanism for ubiquitylating Gβγ.

A simple assay for inhibitors of mycobacterial oxidative phosphorylation

This paper introduces a fluorescence-based assay to detect and distinguish inhibitors of mycobacterial oxidative phosphorylation, showing its utility for identifying new inhibitors.

Structure of a dimeric ABC transporter

This study presents the structure of Ycf1p, a yeast ABC transporter, showing it forms a dimer with distinct phosphorylation and ATPase activity compared to monomers.

Structural basis for inhibition of mycobacterial ATP synthase by squaramides and second generation diarylquinolines

This study investigates how squaramides and diarylquinolines inhibit mycobacterial ATP synthase, revealing structural insights and differences in their mechanisms and effects.

Antibody avidity and multi-specificity combined to confer protection against SARS-CoV-2 and resilience against viral escape

This study develops a multimeric antibody platform (Multabody) that enhances neutralization potency and breadth against SARS-CoV-2 and its variants through avidity and multi-specificity.

Structural basis of V-ATPase VO region assembly by Vma12p, 21p, and 22p

This study reveals how Vma12p, Vma21p, and Vma22p assemble and regulate V-ATPase VO region to prevent premature V1 binding and ensure proper acidification of cellular compartments.

Structure of mycobacterial respiratory Complex I

The paper determines the structure of mycobacterial Complex I using cryoEM, revealing its composition and interactions with menaquinone and a unique lipid.

Structure of V-ATPase from citrus fruit

This study determines the structure of plant V-ATPase from citrus fruit using SidK purification, revealing its ATP:H+ ratio and structural differences from yeast and mammalian enzymes.

Structural basis of mammalian Complex IV inhibition by steroids

This study identifies a steroid-binding site on Complex IV and shows how steroid binding inhibits proton uptake through the K-pathway.

Structure of ATP synthase under strain during catalysis

The paper uses cryoEM to study yeast mitochondrial ATP synthase under strain during ATP-hydrolysis-driven rotation, revealing structural deformation of the peripheral stalk and its role in opposing bending forces.

Detection and quantification of the vacuolar H ATPase using the Legionella effector protein SidK

The paper introduces a new probe derived from SidK to specifically localize and quantify V-ATPases in live and fixed cells, showing their progressive acquisition during phagosome maturation and subdomain distribution.

Cryo-EM of the yeast VO complex reveals distinct binding sites for macrolide V-ATPase inhibitors

The paper determines the structures of the yeast VO complex bound to two macrolide inhibitors, revealing distinct binding sites on the c ring that block proton translocation.

Coordinated conformational changes in the V1 complex during V-ATPase reversible dissociation

The paper uses cryoEM to study conformational changes in yeast V1 complex during V-ATPase dissociation, revealing how subunit C enables reassembly with VO.

CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7

The paper uses cryoEM to study endogenous V-ATPase interactions with mEAK-7 in porcine kidney, revealing structural insights and functional differences compared to yeast.

Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)

This study determines the structure of the mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug telacebec, revealing its mechanism of inhibiting menaquinol oxidation.

The respiratory supercomplex from C. glutamicum

This paper determines the structure of the CIII2CIV2 supercomplex from C. glutamicum using cryo-EM, revealing its architecture and proton transport pathway.

A Family of Glycosylated Macrolides Selectively Target Energetic Vulnerabilities in Leukemia

This study identifies the mitochondrial ATP synthase as the target of apoptolidin family glycomacrolides and demonstrates their efficacy in suppressing leukemia progression.

Rieske head domain dynamics and indazole-derivative inhibition of Candida albicans complex III

This study uses cryoEM to analyze the structure and dynamics of the Rieske head domain in Candida albicans Complex III and shows how Inz-5 alters its conformational equilibrium.

Structure of Ycf1p reveals the transmembrane domain TMD0 and the regulatory R region of ABCC transporters

This paper presents the cryo-EM structure of Ycf1p, revealing the TMD0 domain and parts of the R region in ABCC transporters, and shows how their orientation and interactions influence protein function and disease.

Polymeric assembly of endogenous Tuberous Sclerosis Protein Complex

The paper demonstrates that endogenous Tuberous Sclerosis Protein Complex (pTSC) assembles into filamentous super-structures in mammalian brains.

Selective Targeting of Ras Mutant Cancers via a New Small Molecule

This paper identifies 249C as a small molecule that selectively targets Ras-mutant cancers by inhibiting V-ATPase, disrupting lysosomal acidification and key survival pathways in Ras-driven tumors.

Cryo-EM structure and kinetics reveal electron transfer by 2D diffusion of cytochrome c in the yeast III-IV respiratory supercomplex

This paper uses cryo-EM and kinetic studies to show that cytochrome c transfers electrons via 2D diffusion along the surface of yeast III2IV1/2 supercomplexes, enhancing electron transfer efficiency.

Through-grid wicking enables high-speed cryoEM specimen preparation

This paper introduces a high-speed cryoEM specimen preparation method that reduces blotting times and improves ice film quality for cryoEM imaging.

Structure of mycobacterial ATP synthase with the TB drug bedaquiline

This study determines the structure of mycobacterial ATP synthase with and without bedaquiline, revealing how the drug binds and induces conformational changes to inhibit ATP hydrolysis.

Structural ordering of the Plasmodium berghei circumsporozoite protein repeats by inhibitory antibody 3D11

This study investigates how antibody 3D11 binds to Plasmodium berghei CSP repeats, inducing structural ordering and revealing common mechanisms of antibody evolution against Plasmodium CSP.

CryoEM map of Pseudomonas aeruginosa PilQ enables structural characterization of TsaP

The paper determines the structure of PilQ from Pseudomonas aeruginosa and identifies TsaP as the spoke-forming component, revealing its role in surface-sensing signal transduction.

Electron Event Representation (EER) data enables efficient cryoEM file storage with full preservation of spatial and temporal resolution

The paper introduces EER data format for cryoEM DDD movies, preserving spatial and temporal resolution without increasing file size.