H. Guo

The ability of enzymes to preserve reactive conformations with well-positioned residues during enzyme-catalyzed reactions can be an important factor for efficient catalysis: a case study of CARNMT1

This study uses computational simulations to show that preserving reactive conformations with well-positioned residues is critical for catalytic efficiency in CARNMT1, as demonstrated by comparing wild-type and mutant enzymes.

Reversible ubiquitination of integrated domain controls paired NLR immune receptor complex homeostasis

The paper identifies a ubiquitination-deubiquitination cycle regulating the stability and function of the paired NLR receptor complex RRS1/RPS4 in Arabidopsis.

The maternal vGluT2 and embryonic mGluR3 signaling relay system controls offspring wing dimorphism in pea aphid

This study identifies a signaling relay system involving maternal vGluT2 and embryonic mGluR3 that controls offspring wing dimorphism in pea aphids through transgenerational phenotypic plasticity.

Synthetic organelles enable protein purification in a single operation

This paper introduces PandaPure, a synthetic organelle-based method for protein purification that simplifies and enhances the process in Escherichia coli.

Super homotypic targeting by exosome surface engineering

This paper enhances homotypic targeting by engineering exosome surfaces with lanthanides, significantly improving cell-exosome interactions and enabling new applications in diagnostics and therapy.

Engineering customized viral receptors for various coronaviruses

This paper develops a method to engineer customized viral receptors (CVRs) for various coronaviruses, enabling infection models for viruses lacking known receptors.

Liver cancer initiation is dependent on metabolic zonation but decoupled from premalignant clonal expansion

This study shows that liver cancer initiation depends on metabolic zonation but is not linked to premalignant clonal expansion.

Target-aware Molecule Generation for Drug Design Using a Chemical Language Model

This paper introduces TamGen, a chemical language model for target-aware molecule generation, and shows it produces high-quality compounds with inhibitory activity against Tuberculosis ClpP protease.

Linking root-associated fungal and bacterial functions to root economics

The paper links fungal and bacterial functions in root-associated communities to root economics traits in a subtropical forest.

Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity

The paper presents cryo-EM structures of human SIDT1 and SIDT2, revealing their dimeric architecture and demonstrating low-pH-dependent RNA binding and oligomerization.

Gut microbial genomes with paired isolates from China signify probiotic and cardiometabolic effects

The paper presents a new microbial reference set with paired isolates from China, showing probiotic and cardiometabolic effects on hosts.

Characterization of N-glycosylation and its Functional Role in SIDT1-Mediated RNA Uptake

This study maps N-glycosylation sites in SIDT1 and SIDT2 and shows their role in RNA uptake, cell surface expression, and protein stability.

Metabolic Nutrient Preferences of Vibrio mimicus: Leveraging Nucleotides and Oligopeptides from Yellow Catfish for Enhanced Infectivity

This study identifies host-specific metabolites that enhance Vibrio mimicus infectivity in yellow catfish by providing nutrients like deoxyguanosine.

Contribution of the delta-class glutathione S-transferase to agrochemical adaptation in Apis mellifera

This study characterizes AmGSTD1, a delta-class GST in honey bees, showing its role in agrochemical detoxification and antioxidative stress through structural and functional analysis.

An ACE2 decamer viral trap as a durable intervention solution for current and future SARS-CoV

This paper introduces an ACE2 decamer viral trap that neutralizes multiple SARS-CoV-2 variants and related coronaviruses through inhalation, showing durability and effectiveness in preclinical models.

Cell-based assay to determine Type 3 Secretion System translocon assembly in Pseudomonas aeruginosa using split luciferase

The paper describes a cell-based assay using split luciferase to detect T3SS translocon assembly in Pseudomonas aeruginosa, showing high signal/noise ratio and robustness for inhibitor screening.

Structure-based virtual screening identifies small molecule inhibitors of O-fucosyltransferase SPINDLY

The paper identifies SOFTI, a specific inhibitor of SPY O-fucosyltransferase, through virtual screening and validates its effects on O-fucosylation in Arabidopsis and tomato.

COP9 signalosome complex subunit-7-mediated regulation of cAMP levels contributes to autophagic degradation and pathogenesis of rice blast fungus Magnaporthe oryzae

This study identifies MoCSN7's role in regulating cAMP levels, autophagy, and pathogenesis in Magnaporthe oryzae through functional characterization of COP9 signalosome subunits.

The immune receptor SNC1 monitors helper NLRs targeted by a bacterial effector

The paper shows that SNC1, an immune receptor, guards ADR1-L1 and ADR1-L2 from bacterial effector suppression, enabling plant immunity through sequence diversification and a layered defense system.

Isolation of ACE2-dependent and -independent sarbecoviruses from Chinese horseshoe bats

The paper isolates ACE2-dependent and -independent sarbecoviruses from Chinese horseshoe bats using a new virus isolation protocol.

Cryo-EM structure of DNA polymerase θ helicase domain in complex with inhibitor novobiocin

The paper presents the Cryo-EM structure of DNA polymerase θ helicase domain with and without inhibitor novobiocin, revealing a non-canonical binding pocket and insights into its mechanism.

Cooperative hydrodynamics accompany multicellular-like colonial organization in the unicellular ciliate Stentor

This study shows that hydrodynamic coupling in Stentor colonies enhances feeding efficiency, with asymmetric benefits depending on individual solitary feeding rates.

Large-scale analysis of the N-terminal regulatory elements of the kinase domain in plant receptor-like kinase family

The paper analyzes NRE sequences in RLKs and RLCKs from 528 plants to uncover structural-functional relationships and phosphorylation motifs, revealing their role in kinase regulation.

Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine

This study develops a bivalent vaccine using lineage-mosaic and mutation-patched spike proteins that elicit broad-spectrum antibodies and protect against multiple SARS-CoV-2 variants in hamsters.

Monitoring the intestinal magnetic field with optically pumped atomic magnetometers

This study uses optically pumped magnetometers to measure the intestinal magnetic field of a rabbit, showing their potential for noninvasive disease detection.

Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12

This study identifies BUD31 as a splicing factor that promotes ovarian cancer by maintaining anti-apoptotic BCL2L12 expression through exon inclusion.

Cryo-EM complex structure of active GPR75 with a nanobody

The paper reports the Cryo-EM structure of active GPR75 with a nanobody, revealing its activation mechanism and providing a structural basis for anti-obesity drug discovery.

The effector recognition by synthetic sensor NLR receptors requires the concerted action of multiple interfaces within and outside the integrated domain

This study shows that synthetic sensor NLR receptors require multiple interfaces within and outside their integrated domain to recognize noncorresponding effectors and activate resistance.

Triterpenoid production with a minimally engineered Saccharomyces cerevisiae chassis

This paper engineers Saccharomyces cerevisiae to produce triterpenoids at high titers by modifying an oxidosqualene cyclase, demonstrating a chassis for diverse triterpenoid synthesis.

Validated Preclinical Murine Model for Therapeutic Testing against Multidrug Resistant Pseudomonas aeruginosa

This paper refines a murine model of Pseudomonas aeruginosa infection to predict antimicrobial efficacy against multidrug-resistant strains.