Author

A. Knebel

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 11

Signature Assays, Organisms & Methods

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Approach:experimental assay· 11 Assay:proteomics· 7 Organism:human· 6 Research Focus:cell signaling & trafficking· 4 Research Focus:cell type annotation· 3 Research Focus:drug response / drug discovery· 3 Research Focus:protein folding & dynamics· 3 Research Focus:chromatin & epigenetic regulation· 2

Frequent Co-Authors

V. De Cesare· 4 shared C. Johnson· 3 shared F. Lamoliatte· 3 shared M. Muqit· 3 shared T. Macartney· 3 shared Y. Kulathu· 3 shared D. R. Alessi· 2 shared K. Berndsen· 2 shared

Activity by Year

2017-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 22 mutual co-author links

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4 shared papersBiochemistry

V. De Cesare

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Author profile →

11 papers

Recent·Most cited

Regulation of Human PINK1 ubiquitin kinase by Serine167, Serine228 and Cysteine412 phosphorylation

A. Waddell, H. Ojha, S. Agarwal, C. Clarke, A. Terriente-Felix, H. Zhou, P. Kakade, A. Knebel, A. Shaw, R. Gourlay, J. Varghese, R. Soares, R. Toth, T. Macartney, P. Eyers, , M. Muqit

This study identifies phosphorylation sites in hPINK1 that regulate its activation and ubiquitin interaction, revealing a novel inhibitory mechanism involving pCys412.

bioRxiv · Mar 31, 2023 · PreprintBiochemistry

Discovery and characterization of non-canonical E2 conjugating enzymes

S. A. Abdul Rehman, E. Di Nisio, C. Cazzaniga, O. Antico, A. Knebel, C. Johnson, F. Lamoliatte, R. Negri, M. Muqit, V. De Cesare

The paper identifies and characterizes non-canonical E2 conjugating enzymes that ubiquitylate serine and threonine instead of lysine, revealing their target substrate and catalytic mechanism.

bioRxiv · Mar 5, 2023 · PreprintBiochemistry

Comprehensive approach to study branched ubiquitinchains reveals roles for K48-K63 branches inVCP/p97-related processes

S. M. Lange, M. R. McFarland, F. Lamoliatte, D. Kwasna, L. Shen, I. Wallace, I. Cole, L. A. Armstrong, A. Knebel, C. Johnson, V. De Cesare, Y. Kulathu

This paper develops a pipeline to study branched ubiquitin chains, identifies debranching enzymes, and reveals their roles in VCP/p97 processes and DNA damage responses.

bioRxiv · Jan 10, 2023Biochemistry

Elaboration of a MALDI-TOF Mass Spectrometry-based Assay of Parkin Activity and High-Throughput screening platform for Parkin Activators

R. Traynor, J. Moran, M. U. Stevens, A. Knebel, B. Behrouz, K. Merchant, C. J. Hastie, P. Davies, M. Muqit, V. De Cesare

The paper develops two MALDI-TOF MS-based methods to quantify Parkin E3 ligase activity in vitro, enabling high-throughput screening of Parkin activators.

bioRxiv · Mar 4, 2022 · PreprintBiochemistry

Human UFSP1 is an active protease that regulates UFM1 maturation and UFMylation

D. Millrine, T. Cummings, S. P. Matthews, J. J. Peter, H. Magnussen, T. Macartney, F. Lamoliatte, A. Knebel, Y. Kulathu

The paper identifies UFSP1 as an active protease that matures UFM1 and regulates UFMylation, distinguishing it from UFSP2 in substrate specificity and function.

bioRxiv · Feb 28, 2022Biochemistry

HOIL-1-catalysed ubiquitylation of unbranched glucosaccharides and its activation by ubiquitin oligomers

I. R. Kelsall, E. H. McCrory, Y. Xu, C. L. Scudamore, S. K. Nanda, P. Mancebo-Gamella, N. T. Wood, A. Knebel, S. J. Matthews, P. Cohen

This study shows HOIL-1 ubiquitylates unbranched glucosaccharides and its activity is enhanced by ubiquitin oligomers, preventing toxic polysaccharide accumulation.

bioRxiv · Sep 10, 2021 · PreprintBiochemistry

Structural basis for the specificity of PPM1H phosphatase for Rab GTPases

D. Waschbüsch, K. Berndsen, P. Lis, A. Knebel, Y. P. Lam, D. R. Alessi, A. R. Khan

This paper identifies the structural basis for PPM1H phosphatase specificity for Rab GTPases, showing that a flap domain enables recognition of phosphorylated substrates.

bioRxiv · Feb 17, 2021Biochemistry

Mechanism of activation and regulation of Deubiquitinase activity in MINDY1 and MINDY2

S. A. Abdul Rehman, L. A. Armstrong, S. M. Lange, Y. A. Kristariyanto, T. W. Grawert, A. Knebel, D. Svergun, Y. Kulathu

This paper reveals the mechanism of MINDY1 and MINDY2 deubiquitinase activity, showing how substrate interaction relieves autoinhibition and enables cleavage of K48-linked ubiquitin chains.

bioRxiv · Jan 27, 2021Biochemistry

Human ANKLE1 is a nuclease specific for branched DNA

J. Song, A. D. J. Freeman, A. Knebel, A. Gartner, D. M. J. Lilley

The paper identifies ANKLE1 as a nuclease that specifically cleaves branched DNA structures, suggesting its role in resolving DNA junctions during mitosis.

bioRxiv · Jul 3, 2020Biochemistry

PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins

K. Berndsen, P. Lis, W. Yeshaw, P. S. Wawro, R. S. Nirujogi, M. Wightman, T. Macartney, M. Dorward, A. Knebel, F. Tonelli, S. R. Pfeffer, D. R. Alessi

This study identifies PPM1H as a phosphatase that counteracts LRRK2 signaling by dephosphorylating Rab proteins, suggesting it as a potential therapeutic target for Parkinson's disease.

bioRxiv · Jul 22, 2019Biochemistry

The MALDI TOF E2/E3 ligase assay as an universal tool for drug discovery in the ubiquitin pathway

V. De Cesare, C. Johnson, V. Barlow, J. Hastie, A. Knebel, M. Trost

The paper introduces a MALDI TOF-based assay for high-throughput screening of E2/E3 ligase activity in the ubiquitin pathway, demonstrating its utility for drug discovery.

bioRxiv · Nov 29, 2017Biochemistry