R. Toth

A chemical-genetic system to rapidly inhibit the PP2A-B56 phosphatase

The paper develops a chemical-genetic system to inhibit PP2A-B56 phosphatase by blocking SLiM-binding pockets, revealing its role in kinetochore-microtubule attachments.

Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.

The paper identifies active cytochrome P450s in mouse, plant, and fungal systems using chemical probes and mass spectrometry, showing their subcellular localization and activity-based labeling.

Mechanism of human PINK1 activation at the TOM complex in a reconstituted system

This study identifies TOM20, TOM70, TOM40, and associated subunits as critical for PINK1 activation in a reconstituted yeast system, providing insights for PINK1 activator development.

Chemo-phosphoproteomic profiling with ATR inhibitors berzosertib and gartisertib uncovers new biomarkers and DNA damage response regulators

The paper identifies novel ATR-dependent phospho-proteomic targets and reveals new biomarkers and genome maintenance factors through screening with ATR inhibitors.

Regulation of Human PINK1 ubiquitin kinase by Serine167, Serine228 and Cysteine412 phosphorylation

This study identifies phosphorylation sites in hPINK1 that regulate its activation and ubiquitin interaction, revealing a novel inhibitory mechanism involving pCys412.

Kinome screening identifies integrated stress response kinase EIF2AK1 / HRI as a negative regulator of PINK1 mitophagy signaling

The paper identifies EIF2AK1/HRI as a negative regulator of PINK1 mitophagy signaling through siRNA screening and pharmacological inhibition.

Glioblastoma-instructed microglia transition to heterogeneous phenotypic states with phagocytic and dendritic cell-like features in patient tumors and patient-derived orthotopic xenografts

This study characterizes the tumor microenvironment in glioblastoma patient-derived xenografts and patient tumors, revealing phenotypic heterogeneity in microglia and their functional adaptations.

The Design of Brainstem Interfaces: Characterisation of Physiological Artefacts and Implications for Closed-loop Algorithms

The paper characterizes physiological artefacts in brainstem implants and discusses their implications for closed-loop neuromodulation algorithms.

Interaction with C21ORF2 controls the cellular functions of the NEK1 kinase

This study identifies C21ORF2 as a regulatory subunit of NEK1, showing that their interaction is critical for NEK1 function and that mutations disrupt this interaction, linking it to disease.

Structure-based design and characterization of Parkin activating mutations

The paper identifies three Parkin activating mutations that rescue mitophagy defects in cell-based studies, suggesting therapeutic potential for Parkinson's disease.

Diverse routes of Club cell evolution in lung adenocarcinoma

This study identifies Club cells as a major cell-of-origin for lung adenocarcinoma, revealing two evolutionary trajectories similar to lung regeneration and tumor-specific epigenetic changes.

Global ubiquitylation analysis of mitochondria in primary neurons identifies physiological Parkin targets following activation of PINK1

The paper identifies 49 PINK1-dependent ubiquitylated proteins in mouse neurons and develops a resource to visualize mitochondrial ubiquitin sites.

Candida albicans enhances the progression of oral squamous cell cancrinoma in vitro and in vivo

This study shows that C. albicans promotes OSCC progression in vitro and in vivo by inducing inflammation, oncometabolites, and metastatic gene expression.

Oral epithelial cells distinguish between Candida species with high or low pathogenic potential through miRNA regulation

The paper shows oral epithelial cells respond differently to C. albicans and C. parapsilosis, with miRNA regulation playing a key role in distinguishing their pathogenic potential.

Epilepsy kinase CDKL5 is a DNA damage sensor which controls transcriptional activity at DNA breaks

CDKL5 is a DNA damage-sensing kinase that modulates transcriptional activity near DNA breaks.

Characterization of protease activity of Nsp3 from SARS-CoV-2 and its in vitro inhibition by nanobodies

This study characterizes Nsp3 protease activity from SARS-CoV-2 and identifies nanobodies that inhibit PLpro with nanomolar affinity.

Structural basis of FANCD2 deubiquitination by USP1-UAF1

This study determines the structural basis of FANCD2 deubiquitination by USP1-UAF1, revealing conformational changes and substrate recognition mechanisms.

Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms

This study identifies distinct Rab proteins phosphorylated by LRRK1 and LRRK2, showing they are regulated by different mechanisms and respond differently to inhibitors.

DyNeuMo Mk-1: Design and Pilot Validation of an Investigational Motion-Adaptive Neurostimulator with Integrated Chronotherapy

The paper describes the design and validation of a motion-adaptive neurostimulator with chronotherapy, intended for movement disorders and other applications.

Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL

This study introduces Methyl-COOM, a method to identify cell-of-origin and CLL-specific epigenetic aberrations by analyzing DNA methylation patterns.

PINK1-dependent phosphorylation of Serine111 within the SF3 motif of Rab GTPases impairs effector interactions and LRRK2 mediated phosphorylation at Threonine72

This study shows that PINK1 phosphorylates Rab GTPases at Ser111, which inhibits LRRK2-mediated phosphorylation at Thr72, suggesting PINK1 activators could treat LRRK2-related Parkinson's.

Phagocytic predation by the fungivorous amoeba Protostelium aurantium targets metal ion and redox homeostasis

This study shows that the amoeba Protostelium aurantium targets fungal copper and redox homeostasis during predation, with gene deletions in C. parapsilosis confirming their role in survival against phagocytic killing.

Hypoxia-Induced PIM Kinase and Laminin-Activated Integrin α6 Mediate Resistance to PI3K Inhibitors in Bone-Metastatic CRPC

The paper identifies two survival pathways in bone-metastatic CRPC that confer resistance to PI3K inhibitors and shows combined inhibition of integrin α6β1 and PIM kinase is needed to overcome this resistance.

Random forest-based modelling to detect biomarkers for prostate cancer progression

This study uses a random forest model to identify DNA methylation biomarkers for prostate cancer progression, finding ZIC2 expression loss as an independent prognostic marker.

Specificity for deubiquitination of monoubiquitinated FANCD2 is driven by the N-terminus of USP1

This study identifies the N-terminus of USP1 as critical for its specificity in deubiquitinating FANCD2, but not PCNA or FANCI.

Allosteric network in Ube2T drives specificity for RING E3 catalysed ubiquitin signals.

The paper identifies an allosteric network in Ube2T that enhances FANCD2 ubiquitination while maintaining specificity, and reveals similar networks in other E2s.