A. Typas

Essentiality of LD-Transpeptidation in Agrobacterium tumefaciens

This study identifies the essential role of LD-transpeptidation in Agrobacterium tumefaciens cell wall integrity and growth by analyzing LDTs and their genetic interactions.

Uncovering nitroxoline activity spectrum, mode of action and resistance across Gram-negative bacteria

This study identifies nitroxoline's broad antibacterial activity against Gram-negative bacteria, its mode of action involving metal homeostasis and outer membrane disruption, and resistance mechanisms across multiple species.

Emergent survival and extinction of species within gut bacterial communities

The paper shows how species in gut bacterial communities can survive or go extinct based on resource availability and metabolic interactions, identifying emergent survival and community-driven extinctions.

The killing of human gut commensal E. coli ED1a by tetracycline is associated with severe ribosome dysfunction

The paper examines how tetracycline affects ribosome function in two E. coli strains, showing different outcomes based on the antibiotic's bacteriostatic or bactericidal effect.

Emergence of community behaviors in the gut microbiota upon drug treatment

This study examines how drug treatment affects gut microbiota communities, finding that cross-protection is more common than cross-sensitization, and that metabolic processes contribute to these community behaviors.

Consistency across multi-omics layers in a drug-perturbed gut microbial community

The paper examines consistency and complementarity across five omics layers in a drug-perturbed gut microbial community, finding high agreement in species abundance and functional insights from different data types.

High-throughput profiling of drug interactions in Gram-positive bacteria

This study systematically tests drug combinations in Gram-positive bacteria, identifying many new synergies and antagonisms, including effective combinations against drug-resistant strains.

Transient glycolytic complexation of arsenate enhances resistance in the enteropathogen Vibrio cholerae

This study identifies a novel arsenate resistance mechanism in V. cholerae involving the formation of a transient glycolytic complex to sequester arsenate and prevent its toxic effects.

Modulation of bacterial cell size and growth rate via activation of a cell envelope stress response

This study shows that activating the Rcs phosphorelay in E. coli reduces growth rate and alters FtsZ distribution, with effects mediated through growth-rate changes rather than direct regulation.

Bacterial cell widening alters periplasmic size and activates envelope stress responses

This study shows that changes in bacterial cell width affect periplasmic thickness and activate the Rcs stress response system through RcsF.

Midcell localization of PBP4 of Escherichia coli is modulating the timing of divisome assembly

This paper shows that PBP4 localizes at midcell during septal PG synthesis in E. coli and is important for the timing of divisome assembly.

Phage proteins block and trigger retron toxin/antitoxin systems

The paper identifies phage proteins that block or trigger retron toxin/antitoxin systems in bacteria, revealing their role in anti-phage defense.

Bacterial retrons encode tripartite toxin/antitoxin systems

The paper identifies retrons as a novel family of tripartite toxin/antitoxin systems in bacteria, with the reverse transcriptase and msDNA functioning as antitoxin components.

A dual-mechanism antibiotic targets Gram-negative bacteria and avoids drug resistance

This paper identifies a dual-mechanism antibiotic, SCH-79797, that kills Gram-negative and Gram-positive bacteria with low resistance and outperforms combination treatments.

Global mapping of Salmonella enterica-host protein-protein interactions during infection

The paper maps Salmonella enterica effector-host protein-protein interactions during infection, identifying 421 novel interactions and demonstrating their roles in pathogenesis.

Dissecting the collateral damage of antibiotics on gut microbes

This study characterizes the antibiotic activity spectrum on gut bacteria, revealing species-specific effects and identifying antidotes to mitigate collateral damage.

Systematic localization of Gram-negative bacterial membrane proteins

This study systematically maps membrane protein localization in E. coli using mass spectrometry, identifying discrepancies with existing annotations and providing a reference dataset for Gram-negative bacteria.

Fighting the spread of antibiotic resistance with bacterial competence inhibitors

The paper identifies COM-blockers, which inhibit horizontal gene transfer in S. pneumoniae by disrupting competence without affecting growth or antibiotic activity, offering a strategy to reduce antibiotic resistance spread.

The outer membrane lipoprotein NlpI nucleates hydrolases within peptidoglycan multi-enzyme complexes in Escherichia coli

This study identifies NlpI as a scaffold for PG hydrolases in E. coli, forming multi-enzyme complexes that coordinate PG synthesis and hydrolase activity.