C. Sieben

Single influenza A viruses induce nanoscale cellular reprogramming at the virus-cell interface

This study uses single-molecule microscopy to investigate influenza A virus-induced nanoscale cellular changes at the virus-cell interface, revealing receptor dynamics and actin cortex responses.

The bat Influenza A virus subtype H18N11 induces nanoscale MHCII clustering upon host cell attachment

This study shows that bat influenza A virus H18N11 attaches to MHCII clusters on host cells, causing local clustering of MHCII molecules similar to immunological synapse formation.

An improved workflow for the quantification of orthohantavirus infection using automated imaging and flow cytometry

The paper develops an improved workflow for quantifying orthohantavirus infection using automated imaging and flow cytometry, with an open-source software tool.

Nanoscale organization of the endogenous ASC speck

This study uses super-resolution microscopy to analyze the nanoscale structure of endogenous ASC specks during NLRP3 inflammasome activation, revealing a dense core with extending filaments and changes in density during formation.

Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence

This study shows Pseudomonas aeruginosa PA14 produces R-bodies, which are involved in virulence against nematodes through ribosome cleavage and translational inhibition.

Virtual-SMLM, a virtual environment for real-time interactive SMLM acquisition

Virtual-SMLM provides a virtual environment for real-time interactive single molecule localisation microscopy acquisition, enabling training and validation of automated methods with realistic simulated data.

Nanoscale pattern extraction from relative positions of sparse 3D localisations

The paper introduces a method to extract high-resolution ordered features from localisation microscopy data by analyzing relative molecular positions, applied to infer ultrastructure of various biological structures.

Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation

This study uses super-resolution microscopy to show that influenza A virus binds to sialic acid clusters and activates EGFR clusters on the cell membrane, revealing a mechanism for virus entry.

Multicolor single particle reconstruction of protein complexes

The paper develops a method to reconstruct and co-align multiple proteins from 2D super-resolution fluorescence images, demonstrating it with multi-color 3D reconstructions of proteins in human centrioles and procentrioles.