E. Sezgin

Imaging single particle profiler to study nanoscale bioparticles using conventional confocal microscopy

The paper introduces iSPP, an imaging-based method for analyzing nanoscale bioparticles using conventional confocal microscopy and provides software for its application.

VISION -- an open-source software for automated multi-dimensional image analysis of cellular biophysics

VISION is an open-source software for automated multi-dimensional image analysis of cellular biophysics, enabling high throughput and correlative analysis of biophysical properties.

Plasma membrane order maps functional diversity in immune cells

The paper identifies immune cell subsets with distinct functional behaviors based on plasma membrane order using biophysical profiling and transcriptome analysis.

A cholesterol switch controls phospholipid scrambling by G protein-coupled receptors

The paper shows cholesterol inhibits GPCR-mediated phospholipid scrambling in synthetic vesicles, suggesting a mechanism for maintaining plasma membrane asymmetry.

Discovery of a novel inhibitor of macropinocytosis with antiviral activity

This study identifies virapinib, a novel inhibitor of macropinocytosis that blocks viral entry by SARS-CoV-2, Monkeypox virus, and TBEV without host cell toxicity.

Quantifying fluorescence lifetime responsiveness of environment sensitive probes for membrane fluidity measurements

The paper quantifies how fluorescence lifetime responds to environmental changes in membrane order probes and highlights their sensitivity to biophysical properties.

Quantification of membrane fluidity in bacteria using TIR-FCS

This paper develops a TIR-FCS assay to measure membrane fluidity in live bacteria and finds that cold shock reduces membrane fluidity in Bacillus subtilis.

Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis

The paper shows that ADAM10-mediated removal of mucins during T cell apoptosis enhances macrophage efferocytosis.

Cell Membranes Sustain Phospholipid Imbalance Via Cholesterol Asymmetry

The paper reveals that mammalian plasma membranes have phospholipid imbalances due to cholesterol asymmetry, affecting membrane permeability and protein localization.

Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

This paper shows that displaying GM3g glycans on proteins using specific chemical linkers elicits strong, germline-encoded B cell responses that do not undergo affinity maturation.

Single particle profiler for measuring content and properties of nano-sized bioparticles

The paper introduces a high-throughput method called single particle profiler (SPP) to analyze content and biophysical properties of nanoscale bioparticles, demonstrating its application on mRNA encapsulation, viral binding, and biophysical heterogeneity.

Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties

This study investigates the sensitivity of three environment-sensitive probes to biophysical membrane properties using model systems and simulations.

Influence of the extracellular domains on the dynamic behavior of membrane proteins

This study investigates how extracellular domain properties affect membrane protein dynamics in lipid bilayers by analyzing sorting and mobility in model systems.

STED super-resolution imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes

This study demonstrates the use of NR4A, an exchangeable polarity-sensitive dye, for real-time 3D-STED imaging of membrane dynamics and biophysical properties in live cells.

Synthetic antigen presenting cells reveal the diversity and functional specialisation of extracellular vesicles composing the fourth signal of T cell immunological synapses.

This study uses synthetic antigen-presenting cells to analyze the composition and functional specialization of extracellular vesicles released during T cell immunological synapses.

Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5

The paper characterizes the diffusion and interaction dynamics of PEX5 in the cytosol using advanced microscopy techniques, revealing a novel interaction partner.

A FOXN1 mutation competitively displaces wild-type FOXN1 from higher order nuclear condensates to cause immunodeficiency

This paper identifies a FOXN1 mutation that displaces wild-type FOXN1 from nuclear condensates, leading to immunodeficiency and athymia in heterozygotes.

A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo

A bispecific nanobody neutralizes SARS-CoV-2 and its variant B.1.351 by inducing spike trimer-dimers that prevent ACE2 binding.

The glycosphingolipid inhibitor eliglustat inhibits autophagy in osteoclasts to increase bone mass and reduce myeloma bone disease

Eliglustat inhibits autophagy in osteoclasts, reducing MM bone disease and suggesting its use as a treatment for bone loss disorders.

Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells

The paper investigates how membrane protein aggregation and immobilization affect lipid order in T cells, revealing lipid-mediated recruitment of signaling proteins like Lck.

Lipoprotein particles interact with membranes and transfer their cargo without receptors

This study shows lipoprotein particles transfer cargo to membranes without receptors, using cryo-EM and spectroscopy, revealing receptor-independent mechanisms.

Alpha-ketoglutarate inhibits Regulatory T cell differentiation by coupling lipidome remodelling to mitochondrial metabolism

This study shows that alpha-ketoglutarate inhibits regulatory T cell differentiation by altering lipid metabolism and mitochondrial function.

The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function

This study shows that the chaperonin CCT8 is essential for T cell maturation, selection, and function by maintaining proteostasis, and its absence impairs immune protection against helminths.

Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins

This study shows that anti-GFP nanobodies alter the fluorescence properties, mobility, and organization of GFP-tagged proteins by binding to non-emissive oligomers and promoting their disassembly into monomers.

High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy

This paper demonstrates that high photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy by using advanced detection equipment.

The mammalian plasma membrane is defined by transmembrane asymmetries in lipid unsaturation, leaflet packing, and protein shape

The paper characterizes lipid and protein asymmetries in mammalian plasma membranes and shows their conservation across eukaryotes.

Creating supported plasma membrane bilayers using acoustic pressure

This paper introduces a method to create supported plasma membrane bilayers by bursting GPMVs with ultrasonic pressure, preserving outer leaflet molecule mobility.

Impact of nanoscale hindrances on the relationship between lipid packing and diffusion in model membranes

This study compares lipid packing and diffusion in different membrane models, finding that supported bilayers deviate from the correlation seen in free-standing bilayers.

z-STED imaging and spectroscopy to investigate nanoscale membrane structure and dynamics

This study uses z-STED imaging combined with FCS and spectral imaging to reveal previously unobservable aspects of cellular membrane structure and dynamics at the nanoscale.

Regulation of lipid saturation without sensing membrane fluidity

The paper investigates how cells regulate lipid saturation without sensing membrane fluidity by studying the yeast transcriptional regulator Mga2.