C. Eggeling

Homogeneous large field-of-view and compact iSCAT-TIRF setup for dynamic single molecule measurements

The paper introduces a compact iSCAT-TIRF setup for dynamic single molecule measurements with a large field-of-view and uniform illumination.

Neural Network Informed Photon Filtering Reduces Artifacts in Fluorescence Correlation Spectroscopy Data

This paper introduces a neural network to filter artifacts in fluorescence correlation spectroscopy data, improving analysis of samples with peak artifacts.

A novel PEX14/PEX5 interface links peroxisomal protein import and receptor recycling

This paper identifies a novel PEX14/PEX5 interface involved in peroxisomal protein import and receptor recycling through biochemical and structural analysis.

Deep learning enables fast, gentle STED microscopy

This paper uses deep learning to denoise STED images, reducing photobleaching and photodamage by significantly lowering pixel dwell time.

Quantification of live cell membrane compartmentalization with high-speed single lipid tracking through interferometric Scattering Microscopy

This paper uses interferometric scattering microscopy to analyze lipid diffusion in live cells, finding that compartmentalized diffusion dominates, with larger particles showing tighter confinement.

STED super-resolution imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes

This study demonstrates the use of NR4A, an exchangeable polarity-sensitive dye, for real-time 3D-STED imaging of membrane dynamics and biophysical properties in live cells.

Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5

The paper characterizes the diffusion and interaction dynamics of PEX5 in the cytosol using advanced microscopy techniques, revealing a novel interaction partner.

Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell Microscopy

The paper describes a method to fluorescently label metal-oxide nanoparticles without causing experimental artefacts, ensuring reliable live-cell microscopy for studying nanomaterials.

Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells

The paper investigates how membrane protein aggregation and immobilization affect lipid order in T cells, revealing lipid-mediated recruitment of signaling proteins like Lck.

Nanoscopy on the Chea(i)p

This paper presents a low-cost, 3D-printed super-resolution microscope using a cellphone for imaging and processing, achieving 100nm resolution and enabling live imaging of SARS-CoV-2 viroids.

Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins

This study shows that anti-GFP nanobodies alter the fluorescence properties, mobility, and organization of GFP-tagged proteins by binding to non-emissive oligomers and promoting their disassembly into monomers.

High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy

This paper demonstrates that high photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy by using advanced detection equipment.

Creating supported plasma membrane bilayers using acoustic pressure

This paper introduces a method to create supported plasma membrane bilayers by bursting GPMVs with ultrasonic pressure, preserving outer leaflet molecule mobility.

Background reduction in STED-FCS using coherent-hybrid STED

This study demonstrates that coherent-hybrid STED reduces background noise more effectively than other STED modes in 3D STED-FCS, improving signal quality and resolution.

z-STED imaging and spectroscopy to investigate nanoscale membrane structure and dynamics

This study uses z-STED imaging combined with FCS and spectral imaging to reveal previously unobservable aspects of cellular membrane structure and dynamics at the nanoscale.

Mechanical properties of plasma membrane vesicles correlate with lipid order and viscosity and depend on cell density

The paper investigates how plasma membrane properties like order, viscosity, and bending rigidity change with cell density using giant plasma membrane vesicles.

Nanoscale dynamics of cholesterol in the cell membrane

This study uses advanced fluorescence imaging to examine cholesterol dynamics in cell membranes, finding faster diffusion in live cells compared to model membranes.

Flotillin-dependent lipid-raft microdomains are required for functional phagolysosomes against fungal infections

This study shows that flotillin-dependent lipid rafts are essential for phagolysosomal function against fungal infections, with implications for human susceptibility to aspergillosis.

Adaptive optics allows 3D STED-FCS measurements in the cytoplasm of living cells

This paper introduces an adaptive optics method to correct optical aberrations, enabling 3D STED-FCS measurements in living cell cytoplasm.

HIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly.

Lipid composition but not curvature is a determinant of a low molecular mobility within HIV-1 lipid envelope

The paper investigates the factors affecting molecular mobility on HIV-1 lipid envelopes using STED-FCS, finding that lipid composition and rigidity, not curvature, determine low mobility.

Reconstitution of immune cell interactions in free-standing membranes

The paper presents an in vitro system using synthetic vesicles to study spatiotemporal aspects of immune synapse formation and signaling molecule distribution in T cells.

Complementary studies of lipid membrane dynamics using iSCAT and super-resolved Fluorescence Correlation Spectroscopy

This paper compares STED-FCS and iSCAT techniques for measuring lipid diffusion on SLBs using hybrid fluorescent-gold nanoparticle tags, finding significant differences in diffusion coefficients.

The Organization of Membranes Below 100 nm: Experimental Breakthroughs Occasion a Modeling Manifesto

The paper reviews experimental advances revealing membrane heterogeneity and scale dependence, and calls for simulation methods that can capture these features to better understand membrane organization.

Nanoscale spatio-temporal diffusion modes measured by simultaneous confocal and STED imaging

This study introduces LIESS-FCS, a method that simultaneously measures diffusion in confocal and STED modes to reveal nanoscale diffusion characteristics in live cell membranes.

Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation

This study uses super-resolution microscopy to show that influenza A virus binds to sialic acid clusters and activates EGFR clusters on the cell membrane, revealing a mechanism for virus entry.

Polarity sensitive probes for super resolution STED microscopy

This study evaluates polarity-sensitive dyes in super resolution STED microscopy to probe nanoscale membrane heterogeneity in cell-derived vesicles, live cells, and virus particles.