H. Shroff

Imaging the extracellular matrix in live tissues and organisms with a glycan-binding fluorophore

This paper introduces a fluorophore for imaging extracellular matrix in live tissues and organisms by binding to glycans, enabling nonperturbative and wash-free visualization.

Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells

This study uses live-cell single-molecule fluorescence microscopy to investigate MYO7A trafficking in stereocilia, revealing its dimeric form and movement restrictions mediated by plasma membrane and F-actin interactions.

Altruistic feeding and cell-cell signaling during bacterial differentiation actively enhance phenotypic heterogeneity

This study shows that bacterial differentiation through altruistic feeding and cell-cell signaling increases phenotypic heterogeneity, enhancing population resilience to environmental changes.

Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM)

The paper introduces a polarized dual-view light-sheet microscope to recover 3D orientations and positions of fluorescent dipoles, validated with experiments on biological samples.

Phase diversity-based wavefront sensing for fluorescence microscopy

This paper introduces a phase diversity-based wavefront sensing method for fluorescence microscopy to correct optical aberrations efficiently and accurately.

Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy

This paper introduces a deep learning method to correct optical aberrations in fluorescence microscopy, improving image quality and enabling better downstream analysis.

Cell division machinery drives cell-specific gene activation during bacterial differentiation

The paper shows that the cell division machinery in Bacillus subtilis drives asymmetric localization of DivIVA and SpoIIE, leading to cell-specific activation of σF during sporulation.

Three-dimensional structured illumination microscopy with enhanced axial resolution

The paper introduces two methods to enhance axial resolution in 3D SIM microscopy, achieving isotropic resolution with minimal hardware changes and using deep learning for improved imaging.

Label-free cleared tissue microscopy and machine learning for 3D histopathology of biomaterial implants

This study uses cleared tissue microscopy and machine learning to analyze 3D histopathology of biomaterial implants and their interaction with tissue microenvironments.

Coiling of cellular protrusions around extracellular fibers

This study combines theory and experiments to show that cellular protrusions coil around round fibers but not elliptical ones, driven by membrane curvature and adhesion energies.

Incorporating the image formation process into deep learning improves network performance in deconvolution applications

This paper introduces RLN, a deep learning method for 3D fluorescence microscopy deconvolution that improves speed, performance, and generalizability compared to existing methods.

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein

The paper analyzes sequence and structural variability in the SARS-CoV-2 nucleocapsid protein, finding high mutability and structural plasticity linked to enhanced viral assembly efficiency.

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes

This paper applies RCAN to enhance fluorescence microscopy image volumes, achieving denoising and resolution improvements across multiple microscopy techniques.

Multiview super-resolution microscopy

This paper introduces a multiview super-resolution microscopy approach that enhances confocal microscopy resolution and reduces phototoxicity across multiple scales.

A polymer gel index-matched to water enables diverse applications in fluorescence microscopy

This paper introduces a polymer gel matched to water's refractive index, enabling high-resolution imaging of living cells and organisms in thick layers.

Signaling through polymerization and degradation: Analysis and simulations of T cell activation mediated by Bcl10

This paper quantifies Bcl10 filament dynamics in T cell activation using microscopy and simulations, showing degradation at filament ends controls response timing.

Nanoscale pattern extraction from relative positions of sparse 3D localisations

The paper introduces a method to extract high-resolution ordered features from localisation microscopy data by analyzing relative molecular positions, applied to infer ultrastructure of various biological structures.

Accelerating iterative deconvolution and multiview fusion by orders of magnitude

This paper introduces faster deconvolution and multiview fusion methods, achieving speedups of 10 to several thousand-fold over previous approaches.

N-WASP regulates the mobility of the B cell receptor and co-receptors during signaling activation

This study uses single molecule imaging and machine learning to show that N-WASP regulates BCR and co-receptor mobility during signaling, affecting actin dynamics and B cell responses.

Single-shot super-resolution total internal reflection fluorescence microscopy

This paper introduces a method combining instant structured illumination microscopy with TIRF to achieve higher resolution and faster imaging of live samples near the coverslip.

A scheme for 3-dimensional morphological reconstruction and force inference in the early C. elegans embryo

This study presents a method for 3D morphological reconstruction and force inference in early C. elegans embryos, showing deviations of 7% and 10% from force balance and Young-Laplace relations.

Reflective imaging improves resolution, speed, andcollection efficiency in light sheet microscopy

This paper introduces a reflective imaging technique that enhances resolution, speed, and collection efficiency in light sheet microscopy without altering the microscope hardware.