S. Manley

Spatiotemporal Modeling of Mitochondrial Network Architecture

This study models mitochondrial network dynamics using simulations and analytic models to show how local parameters control network morphology and remodeling rates.

Surface passivation and functionalization for interferometric scattering microscopy

This paper describes surface passivation and functionalization methods for interferometric scattering microscopy to reduce non-specific binding and enable single-molecule experiments.

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

The paper tracks replisome dynamics in Caulobacter crescentus and finds that chromosome organization influences replisome colocalization and splitting, with replication-transcription conflicts affecting replication speed and replisome decoupling.

Label-free imaging of DNA interactions with 2D materials

This study uses iSCAT microscopy to observe DNA interactions with 2D materials in solution, finding enhanced binding at engineered defects.

Polyphosphate affects cytoplasmic and chromosomal dynamics in nitrogen-starved Pseudomonas aeruginosa

This study shows that polyphosphate reduces cytoplasmic mobility and chromosomal accessibility in nitrogen-starved Pseudomonas aeruginosa, potentially explaining its pleiotropic effects.

Event-driven acquisition for content-enriched microscopy

The paper introduces an event-driven acquisition framework that enhances microscopy by capturing dynamic biological events with optimized imaging rates.

Nanoscale organization of the endogenous ASC speck

This study uses super-resolution microscopy to analyze the nanoscale structure of endogenous ASC specks during NLRP3 inflammasome activation, revealing a dense core with extending filaments and changes in density during formation.

Distinct molecular signatures of fission predict mitochondrial degradation or proliferation

The paper identifies two distinct fission types in mitochondria, one leading to degradation and the other to proliferation, both Drp1-mediated but using different adaptors and pre-constriction mechanisms.

Mitochondrial membrane tension governs fission

The paper investigates mitochondrial fission dynamics, finding that membrane tension influences the likelihood of constrictions dividing.

Homogeneous multifocal excitation for high-throughput super-resolution imaging

This paper develops a high-throughput super-resolution imaging technique to enable large-scale, high-resolution imaging of biological structures.

Virtual-SMLM, a virtual environment for real-time interactive SMLM acquisition

Virtual-SMLM provides a virtual environment for real-time interactive single molecule localisation microscopy acquisition, enabling training and validation of automated methods with realistic simulated data.

Nanoscale pattern extraction from relative positions of sparse 3D localisations

The paper introduces a method to extract high-resolution ordered features from localisation microscopy data by analyzing relative molecular positions, applied to infer ultrastructure of various biological structures.

Mitochondrial RNA granules are fluid condensates, positioned by membrane dynamics

This study investigates mitochondrial RNA granules (MRGs), finding they are fluid condensates that undergo fusion and are positioned by membrane dynamics.

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size

The paper shows that Pom1 gradients in S. pombe cells are regulated by multi-phosphorylation and clustering, adjusting mid-cell levels based on cell size.

Design Principles for Autonomous Illumination Control in Localization Microscopy

This paper designs an autonomous illumination control system for localization microscopy that eliminates the need for sample-specific inputs.

Constriction rate modulation can drive cell size control and homeostasis in C. crescentus

This study shows that constriction rate in C. crescentus affects cell size control and homeostasis by regulating elongation during division and compensating for pre-constriction variation.

Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation

This study uses super-resolution microscopy to show that influenza A virus binds to sialic acid clusters and activates EGFR clusters on the cell membrane, revealing a mechanism for virus entry.

Multicolor single particle reconstruction of protein complexes

The paper develops a method to reconstruct and co-align multiple proteins from 2D super-resolution fluorescence images, demonstrating it with multi-color 3D reconstructions of proteins in human centrioles and procentrioles.