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18 papers
Recent·Most cited
C. Catapano, M. S. Dietz, J. Kompa, S. Jang, P. Freund, K. Johnsson, M. Heilemann
This paper introduces a labeling method for long-term single-molecule tracking in living cells and demonstrates its application to study EGFR dynamics.
bioRxiv · Jul 11, 2024Biophysics
C. S. Katsanos, L. Tran, N. Hoffman, L. R. Roust, E. A. De Filippis, L. J. Mandarino, K. Johnsson, M. Belohlavek, M. R. Buras
This study examines how insulin affects lipid extraction from plasma in lean and obese individuals, finding impaired lipid partitioning away from muscle in obese insulin-resistant humans.
bioRxiv · Jun 11, 2024Physiology
T. Liu, J. Kompa, J. Ling, N. Lardon, Y. Zhang, J. Chen, L. Reymond, P. Chen, M. Tran, Z. Yang, H. Zhang, Y. Liu, S. Pitsch, P. Zou, L. Wang, , Z. Chen
This paper introduces Gentle Rhodamines with reduced phototoxicity for live-cell imaging, enabling time-lapse and voltage imaging with less cellular damage.
bioRxiv · Feb 6, 2024Cell Biology
L. Hellweg, M. Pfeifer, L. Chang, M. Tarnawski, A. Bergner, J. Kress, J. Hiblot, J. Reinhardt, K. Johnsson, P. Leippe
The paper develops a biosensor, iAspSnFR, to measure intracellular aspartate levels in live cells and demonstrates its utility in studying metabolic pathways and cancer biology.
bioRxiv · May 4, 2023Cell Biology
L. Hellweg, A. Edenhofer, L. Barck, M.-C. Huppertz, M. S. Frei, M. Tarnawski, A. Bergner, B. Koch, K. Johnsson, J. Hiblot
The paper introduces a method to create multicolor biosensors with large dynamic ranges using FRET pairs based on fluorescent proteins and HaloTag.
bioRxiv · Nov 29, 2022Biochemistry
M. Glogger, D. Wang, J. Kompa, A. Balakrishnan, J. Hiblot, H.-D. Barth, K. Johnsson, M. Heileman
This paper demonstrates 6-target STED microscopy using exchangeable labels, improving multiplexing capabilities and reducing photobleaching in live-cell imaging.
bioRxiv · Jul 2, 2022 · PreprintBiophysics
J. Kompa, J. Bruins, M. Glogger, J. Wilhelm, M. S. Frei, M. Tarnawski, E. d'Este, M. Heilemann, J. Hiblot, K. Johnsson
The paper introduces exchangeable HaloTag ligands for multi-modal super-resolution fluorescence microscopy, enabling dual-color imaging techniques like PAINT and STED.
bioRxiv · Jun 20, 2022 · PreprintBiochemistry
M. S. Frei, B. Koch, J. Hiblot, K. Johnsson
The paper demonstrates that eight pairwise combinations of 18 synthetic fluorescent probes are suitable for fluorescence lifetime multiplexing in mammalian cells, enabling imaging of up to six biological targets.
bioRxiv · Jan 15, 2022Cell Biology
N. Lardon, L. Wang, A. Tschanz, P. Hoess, M. Tran, E. D'Este, J. Ries, K. Johnsson
This paper describes a method to systematically tune the spirocyclization equilibrium of rhodamines to create probes for different super-resolution microscopy techniques.
bioRxiv · May 20, 2021Cell Biology
E. Kemter, A. Müller, M. Neukam, A. Ivanova, N. Klymiuk, S. Renner, K. Yang, J. Broichhagen, M. Kurome, V. Zakhartchenko, B. Kessler, K.-P. Knoch, M. Bickle, B. Ludwig, K. Johnsson, , M. Solimena
This study develops a transgenic pig model with SNAP-tagged insulin to investigate in vivo insulin secretory granule dynamics and turnover.
bioRxiv · Apr 23, 2021Cell Biology
J. Wilhelm, S. Kuehn, M. Tarnawski, G. Gotthard, J. Tuennermann, T. Taenzer, J. Karpenko, N. Mertes, L. Xue, U. Uhrig, J. Reinstein, J. Hiblot, K. Johnsson
The paper compares the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag, and CLIP-tag to guide their optimal use in bioimaging and biotechnology.
bioRxiv · Apr 13, 2021Biochemistry
M. S. Frei, M. Tarnawski, J. Roberti, B. Koch, J. Hiblot, K. Johnsson
HaloTag9 improves fluorophore performance with higher brightness and fluorescence lifetime, enabling live-cell multiplexing and biosensor generation.
bioRxiv · Apr 1, 2021Biochemistry
M. Umebayashi, S. Takemoto, L. Reymond, M. Sundukova, R. Hovius, A. Bucci, P. A. Heppenstall, H. Yokota, K. Johnsson, H. Riezman
This paper develops a method to monitor the local membrane environment around the insulin receptor in living cells and finds it is dynamic, heterogeneous, and changes with receptor activation.
bioRxiv · Dec 23, 2020Cell Biology
M. Neukam, P. Sala, A.-D. Brunner, K. Ganss, A. Palladini, M. Grzybek, J. Vasiljevic, J. Broichhagen, U. Coskun, K. Johnsson, T. Kurth, M. Mann, M. Solimena
The paper purifies age-distinct insulin secretory granules from INS-1 cells and identifies age-related changes in their lipid composition and membrane properties.
bioRxiv · Jun 3, 2020Cell Biology
L. WANG, M. Tran, E. D Este, J. Roberti, B. Koch, L. Xue, K. Johnsson
The paper develops a strategy to create fluorogenic probes for live-cell nanoscopy with high cell permeability and low background signal.
bioRxiv · Jul 2, 2019Biochemistry
J. Ast, A. Arvaniti, N. H. F. Fine, D. Nasteska, F. B. Ashford, Z. Stamataki, Z. Koszegi, A. Bacon, S. Trapp, B. J. Jones, B. Hastoy, A. Tomas, C. Reissaus, A. K. Linnemann, E. D'Este, , D. J. Hodson
The paper introduces LUXendin peptides that label and visualize endogenous GLP1R with high specificity, revealing receptor organization and dynamics.
bioRxiv · May 8, 2019Cell Biology
M. S. Frei, P. Hoess, M. Lampe, B. Nijmeijer, M. Kueblbeck, J. Ellenberg, J. Ries, S. Pitsch, L. Reymond, K. Johnsson
This paper introduces a new photoactivatable fluorophore that enables live-cell super-resolution microscopy without caging groups or side-products.
bioRxiv · May 4, 2019Biophysics
H. Farrants, A. Acosta Ruiz, V. A. Gutzeit, D. Trauner, K. Johnsson, J. Levitz, J. Broichhagen
This paper introduces a method to reversibly control GPCR activity using photoswitchable ligands tethered via SNAP-tagged nanobodies.
bioRxiv · Feb 15, 2018Pharmacology & Toxicology