M. Digman

ESPRESSO: Spatiotemporal omics based on organelle phenotyping

ESPRESSO provides high-dimensional spatiotemporal phenotyping of organelles using fluorescent labeling and advanced microscopy, enabling analysis of cell states, stress response, and immune cell polarization.

The microenvironment dictates glycocalyx construction and immune surveillance

This study shows that the physical properties of the microenvironment influence glycocalyx composition and immune surveillance in vitro using physiologically relevant models.

Building Fluorescence Lifetime Maps Photon-by-photon by Leveraging Spatial Correlations

This paper introduces a doubly nonparametric framework for FLIM that learns fluorescent species and absolute lifetime maps while addressing spatial correlations and low photon counts.

Multiplexed bioluminescence microscopy via phasor analysis

This paper introduces a method combining bioluminescence with phasor analysis to rapidly differentiate multiple luciferase reporters and resonance energy transfer processes in time-lapse imaging.

NAD consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival

The paper shows PARP1 activation in response to DNA damage causes NAD+ depletion, leading to a metabolic shift to oxidative phosphorylation, which supports cell survival.