M. Vignuzzi

Fitness adaptations of Japanese encephalitis virus in pigs following vector-free serial passaging

This study shows that serial passaging of JEV in pigs leads to fitness adaptations and amino acid changes, but not increased direct transmission.

IRF8 regulates efficacy of therapeutic anti-CD20 monoclonal antibodies

This study identifies IRF8 as a regulator of CD20 expression and anti-CD20 antibody efficacy through CRISPR screening and functional validation in B cells.

The E2 glycoprotein holds key residues for Mayaro virus adaptation to the urban Aedes aegypti mosquito

This paper identifies a mutation in the E2 glycoprotein of Mayaro virus that enhances transmission in Aedes aegypti but reduces replication in human cells, suggesting a trade-off in host adaptation.

Identification of DAXX As A Restriction Factor Of SARS-CoV-2 Through A CRISPR/Cas9 Screen

The paper identifies DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen, showing it limits viral replication independently of SUMOylation.

Exploring zebrafish larvae as a COVID-19 model: probable SARS-COV-2 replication in the swim bladder

This study tests SARS-CoV-2 replication in zebrafish larvae, finding limited infection except in the swim bladder, which shows some viral RNA and nucleoprotein expression.

Phospholipidosis is a shared mechanism underlying the in vitro antiviral activity of many repurposed drugs against SARS-CoV-2

Host PDZ-containing proteins targeted by SARS-Cov-2

The paper identifies sixteen human PDZ binders of SARS-CoV-2 proteins E, 3a, and N, showing significant interactions and potential roles in viral replication and immune evasion.

BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2

This study identifies BRD2 as a regulator of ACE2 transcription and shows that BRD2 inhibitors block SARS-CoV-2 infection in human cells and animal models.

Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets with therapeutic potential

This study identifies novel SARS-CoV-2 protease cleavage sites and cellular targets using mass spectrometry, showing that targeting these proteins can inhibit viral replication.

Comparative analysis of neuroinvasion by Japanese encephalitis virulent and vaccine strains in an in cellulo model of human blood-brain barrier

This study compares the neuroinvasion ability of JEV virulent and vaccine strains across an in cellulo human BBB model, showing differences in infectious particle crossing.

Modeling cell infection via virus-producing cells rather than free infectious virus significantly improves fits of an in vitro viral kinetic data

This study improves viral kinetic modeling by showing that cell-to-cell transmission, not free virus, better explains chikungunya and Zika virus in vitro infection dynamics.

DISSEQT - DIStribution based modeling of SEQuence space Time dynamics

DISSEQT models sequence space dynamics of evolving microbial populations, revealing low-dimensional genetic spaces and predicting phenotype from genotype with high accuracy.