M. Kampmann

Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes

This study shows tau fibrils enter cells via endocytosis, accumulate in lysosomes, cause membrane damage, and nucleate cytosolic tau at lysosomes.

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

The paper uses CRISPR screens in iPSC-derived neurons to identify factors controlling tau aggregation, finding new pathways and potential therapeutic targets for tauopathies.

Pharmacologic Activation of a Compensatory Integrated Stress Response Kinase Promotes Mitochondrial Remodeling in PERK-deficient Cells

This study shows that halofuginone can compensate for PERK signaling deficiencies by promoting adaptive mitochondrial remodeling, while BtdCPU causes mitochondrial fragmentation.

A genome-wide CRISPR interference screen using an engineered trafficking biosensor reveals a role for RME-8 in opioid receptor regulation

The paper uses a CRISPR interference screen with a trafficking biosensor to identify genes regulating opioid receptor trafficking, finding RME-8 as a novel regulator.

Parallel CRISPR-Cas9 screens reveal mechanisms of PLIN2 and lipid droplet regulation

The paper uses CRISPR-Cas9 screens to identify genes regulating PLIN2 and lipid droplet abundance, revealing new mechanisms and creating a resource for lipid metabolism research.

An E3 ligase network engages GCN1 to promote elongation factor-1α degradation on stalled ribosomes

The paper identifies an E3 ligase network that promotes eEF1A degradation on stalled ribosomes through interaction with GCN1.

Number and proportion of P. falciparum gametocytes vary from acute infection to chronic parasite carriage despite unaltered sexual commitment rate

The paper compares gametocyte levels and sexual commitment in P. falciparum infections during dry and wet seasons, finding increased mature gametocytes in asymptomatic dry season carriers without changes in sexual commitment.

mTOR activation induces endolysosomal remodeling and nonclassical secretion of IL-32 via exosomes in inflammatory reactive astrocytes

Functional multi-omics reveals genetic and pharmacologic regulation of surface CD38 in multiple myeloma

This study uses multi-omics approaches to identify regulators of CD38 surface expression in multiple myeloma and assess the impact of therapies on CD38 levels and signaling.

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells

PIKfyve inhibition blocks endolysosomal escape of α-synuclein fibrils and spread of α-synuclein aggregation

The paper identifies PIKfyve inhibition as a strategy to block α-synuclein fibril escape from lysosomes, reducing aggregation spread in a human cell model.

BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2

This study identifies BRD2 as a regulator of ACE2 transcription and shows that BRD2 inhibitors block SARS-CoV-2 infection in human cells and animal models.

A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling

The paper develops a CRISPR interference screen to identify regulators of GPCR/cAMP signaling, identifying 45 strong and 50 weaker regulators, and validates seven novel mediators.

Image-based pooled whole genome CRISPR screening for Parkin and TFEB subcellular localization

The paper introduces AI-Photoswitchable Screening (AI-PS) to identify genes affecting subcellular localization, validating factors involved in TFEB relocation.

CRISPR-based screens uncover determinants of immunotherapy response in Multiple Myeloma

This study uses CRISPR screens to identify mechanisms increasing BCMA expression and enhancing anti-myeloma efficacy of BCMA-targeted therapies in multiple myeloma.

Pharmaceutical-grade Rigosertib is a Microtubule-destabilizing Agent

This paper shows pharmaceutical-grade rigosertib, with high purity, causes microtubule destabilization in cells, confirming its mechanism of action against cancer cells.

Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation

The paper identifies ESCRT pathway components as regulators of tau aggregation propagation by enabling endolysosomal escape of tau seeds.

Mitochondrial dysfunction is signaled to the integrated stress response by OMA1, DELE1 and HRI

The paper identifies OMA1, DELE1, and HRI as a pathway that relays mitochondrial dysfunction to the integrated stress response, showing its role in activating eIF2 kinase activity and potential therapeutic relevance.

CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons

This study develops a CRISPRi platform for genetic screens in human iPSC-derived neurons, identifying gene knockdown effects on survival, transcriptomics, and morphology.