Y. Lei

Human cell surface-AAV interactomes identify LRP6 as blood-brain-barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder

De novo cancer mutations frequently associate with recurrent chromosomal abnormalities during long-term human pluripotent stem cell culture

This study tracks genetic changes in human pluripotent stem cells over time, finding that recurrent chromosomal gains drive the accumulation of other mutations.

Genomic evolution reshapes cell type diversification in the amniote brain

The paper creates a single-cell atlas of amniote brains to study cell type diversification and identifies species-specific gene expression patterns and novel cell subtypes.

Caspase-11 drives macrophage hyperinflammation in models of Polg-related mitochondrial disease

This study identifies caspase-11 as a driver of macrophage hyperinflammation in Polg-related mitochondrial disease models, linking IFN-I signaling to exacerbated infection outcomes.

Rhizobial effector NopM ubiquitinates Nod factor receptor NFR5 and promotes rhizobial infection in Lotus japonicus

This study identifies NopM as a rhizobial effector that ubiquitinates NFR5, enhancing infection of Lotus japonicus by modulating Nod factor signaling.

Phase Contrast Tomography (PCT)

This paper introduces phase contrast tomography (PCT) for 3D phase imaging, demonstrating its high resolution and stability for imaging microspheres and sub-organelles.

The Rhizobial effector NopT targets Nod factor receptors to regulate symbiosis in Lotus japonicus

The paper shows that the rhizobial effector NopT interacts with Nod factor receptors NFR1 and NFR5 in Lotus japonicus to regulate symbiosis by cleaving NFR5 and being counteracted by NFR1 phosphorylation.

Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway

This study shows that inhibiting OGT activates type I interferon-dependent antitumor immunity by enhancing the cGAS-STING pathway through genomic instability.

Adherent reformed islets: a long-term primary cell-based platform for exploring mouse and human islet biology.

This study develops a long-term primary cell-based platform called reformed islets to study mouse and human islet biology, showing they retain key physiological and functional features of native islets.

Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity

This study shows that 1q gain in hESCs increases MDM4 expression, giving a growth advantage during differentiation by reducing DNA damage sensitivity and altering differentiation capacity.

SALL3 mediates the loss of neuroectodermal differentiation potential in human embryonic stem cells with chromosome 18q loss

This study shows that loss of chromosome 18q in human embryonic stem cells impairs neuroectoderm commitment and cardiac progenitor differentiation through SALL3 downregulation.

CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis

This study compares the tumorigenic potential of MOLT4 and JURKAT T-ALL cell lines, finding that CCR9 overexpression enhances cholesterol biosynthesis and promotes T-ALL progression.

TERT accelerates BRAF mutant-induced thyroid cancer dedifferentiation and progression by regulating ribosome biogenesis

This study shows TERT promotes thyroid cancer dedifferentiation and progression by enhancing ribosome biogenesis, and inhibiting rRNA synthesis can reverse this process.

Ensemble Processing and Synthetic Image Generation For Abnormally Shaped Nuclei Segmentation

This paper proposes a system for segmenting abnormally shaped nuclei using synthetic data and ensemble strategies, achieving better performance than existing methods.

cpSRP54 and FtsH cooperate thylakoid membrane associated proteostasis during de-etiolation in Arabidopsis thaliana

The paper shows cpSRP54 and FtsH work together in thylakoid membrane protein quality control during de-etiolation in Arabidopsis, and provides a trackable marker for monitoring their activities.

Mesenchymal stromal cells and alpha-1 antitrypsin have a strong synergy in modulating inflammation and its resolution

This study shows that combining mesenchymal stromal cells and alpha-1 antitrypsin significantly improves inflammation modulation and resolution in vitro and in a mouse model.

Regulation of multidrug efflux pumps by TetR family transcriptional repressor negatively affects secondary metabolism in Streptomyces coelicolor A3(2)

This study identifies TFR-ABC transporter gene sets in Streptomyces coelicolor that regulate secondary metabolism and antibiotic resistance by repressing efflux pump expression.

Aryl hydrocarbon receptor utilises cellular zinc signals to maintain the gut epithelial barrier

This study shows that AHR activation by dietary ligands improves gut barrier function via zinc-dependent pathways, highlighting the role of zinc in AHR signaling and its protective effects against IBD.

Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity

This study shows that ZBP1 and cGAS cooperate to detect mitochondrial DNA, leading to cardiotoxicity, and that blocking this interaction protects against heart damage.

Comparative genomics of orobanchaceous species with different parasitic lifestyles reveals the origin and stepwise evolution of plant parasitism

This study compares genomes of orobanchaceous plants with different parasitic lifestyles to trace the evolutionary steps of plant parasitism.

Broadly neutralizing and protective nanobodies against diverse sarbecoviruses

The paper identifies nanobodies with broad neutralizing activity against multiple sarbecoviruses and shows their protective efficacy in a mouse model.

Global Spatial Transcriptome of Macaque Brain at Single-Cell Resolution

The paper creates a 3-D cell type atlas of the monkey brain using single-cell spatial transcriptomics and identifies molecular markers for neocortical layers and subregions.

Activation of the cGAS-STING innate immune response in cells with deficient mitochondrial topoisomerase TOP1MT

This study links TOP1MT deficiency and the P193L variant to mtDNA release and cGAS-STING activation, contributing to autoimmune disease susceptibility.

Yeast biopanning for detecting antibody binding to site-specific phosphorylations in tau

This paper develops a yeast biopanning platform to identify phospho-site specific antibodies against tau, demonstrating robust detection of phosphorylated tau peptides.

Cell transcriptomic atlas of the non-human primate Macaca fascicularis

The paper presents a single-cell transcriptomic atlas of Macaca fascicularis, identifying cell interactions and disease associations.

Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells

This study uses single-cell RNA sequencing to identify five MSC subpopulations, revealing distinct developmental trajectories and functional roles in self-renewal and immunoregulation.

Engineered human brown adipocyte microtissues improved glucose and insulin homeostasis in high fat diet-induced obese and diabetic mice

This study develops 3D human brown adipose microtissues that improve glucose and insulin homeostasis in obese and diabetic mice.

Metallothionein-2A protect Cardiomyocytes from Ischemia/ Reperfusion through inhibiting p38

This study shows that MT2A reduces p38 activation in cardiomyocytes during ischemia/reperfusion, thereby protecting against I/R injury.

Gains of 12p13.31 delay WNT-mediated initiation of hPSC differentiation and promote residual pluripotency in a cell cycle dependent manner

This study shows that gains of 12p13.31 in hPSC delay WNT signaling and promote residual pluripotency through NANOG and GDF3, dependent on cell cycle position during differentiation.

Isolating and Cryo-Preserving Pig Skin Cells for Single Cell RNA Sequencing Study

This study develops a method for isolating and cryo-preserving pig skin cells for scRNA-Seq, enabling the identification of major skin cell types.