cryo-EM

This study shows how a plant pathogen effector blocks the stepwise assembly of a helper NLR resistosome by stabilizing an intermediate state.

This paper uses cryo-EM to reveal the dynamic conformational landscape and C-terminal gating mechanism of a mycobacterial class Ib ribonucleotide reductase, showing multiple subunit association states and structural flexibility.

The paper establishes a tripartite co-culture system to study defensive mutualism between a Nanobdellati symbiont and its host, revealing glycan-mediated protection against viral infection.

This study designs and characterizes de novo protein binders that specifically target the closed state of HSP90, showing their structural accuracy and functional properties.

This study identifies a specialized proteasome structure in mature mammalian sperm using cryo-EM, revealing that PAC1/2 repurposes from a chaperone to a stable regulator, defining a hyperactive 20S variant.

This study reveals how chaperones guide the conformational transitions of the 26S proteasome base subcomplex through intermediates to achieve ATPase activity.

This study determines the cryo-EM structure of TASK-1 at low pH and shows how protonation of an extracellular histidine leads to channel closure, with mutations affecting proton sensitivity.

This study uses cryo-EM to show doxycycline binds to the ribosome exit tunnel in C. burnetii and E. coli, revealing new mechanisms of ribosome inhibition.

This study determines the structure of multi-channel connexin-46/50 gap junction assemblies, revealing lipid-mediated channel organization and refined single-channel structure.

The paper introduces a robotic system for cryo-EM grid preparation that improves sample efficiency and reconstruction quality.

This study reveals the conformational dynamics of zebrafish Abcb4 using cryo-EM, showing its open-and-close motion and substrate-binding flexibility, which may explain its polyspecificity.

The paper determines the structure of an active FlhA ring and shows how it couples substrate specificity to export engine activation through linker-mediated docking.

This study determines how MRP2 recognizes diverse drugs structurally, showing overlapping ligand binding with different interactions.

This study reports cryo-EM structures of P2X2R in lipid nanodiscs, revealing how ATP binding and lipid interactions regulate channel opening and desensitization.

AlignPCA-2D improves 2D classification in cryo-EM by using PCA-reduced Euclidean vector alignment, achieving competitive accuracy with lower computational cost.

The paper describes the structure and function of a membrane-spanning E3 ubiquitin ligase complex, revealing its role in developmental processes and linking mutations to human birth defects.

The paper determines the mechanism of teichoic acid transport by the flippase TacF in Streptococcus pneumoniae using cryo-EM and computational methods.

The paper introduces PP7 virus-like particles as a standardized benchmark for cryo-electron tomography, enabling rapid validation and resolution assessment of tomographic pipelines.

This study presents cryoEM structures of PaMprF from Pseudomonas aeruginosa, revealing its dimeric architecture and dynamic elements involved in lipid modification.

This study shows cGAS forms a protein-dsDNA mesh that enables dsDNA-specific and length-dependent activation through higher-order assembly.

The paper presents cryo-EM structures of Escherichia coli cytochrome bd-I to elucidate its mechanism of quinol oxidation and inhibition, identifying key residues and structural changes involved in catalysis and drug binding.

The paper identifies components of mammalian sperm nuclear vacuoles using cryo-ET and AlphaFold docking, revealing proteasomes, PA200, and ferritin, and their structural roles.

The paper reports cryo-EM structures of the Zn2+ ABC transporter ZnuB-ZnuC from E. coli, revealing a self-regulating mechanism via a zinc sensor.

The paper uses cryo-ET and fluorescence microscopy to study ER exit sites in human cells, revealing COPII and COPI vesicle structures and resolving their origin and organisation.

The paper identifies an intermediate conformation of ALC1 bound to a nucleosome's minor super-groove, revealing structural insights into its activation mechanism.

This study uses cryo-EM to reveal how Lmod2 stabilizes actin subunits at the pointed end of filaments and promotes elongation by introducing strain.

The paper reports cryo-EM structures of ArsB, revealing its mechanism of metalloid transport and H+/metalloid antiport.

StructAgent automates cryo-EM model building and refinement with a multi-agent system, enabling auditable workflows while preserving expert control.

This study reveals the structural mechanism of electron transfer in iNOS using cryo-EM, showing how FMND moves to shuttle electrons from FAD to haem via W366.

The paper determines the structure of the ChlG2HliD2 complex using cryo-EM to elucidate chlorophyll synthesis and photoprotection mechanisms.