D. Bulkley

High-resolution single-particle imaging at 100-200 keV with the Gatan Alpine direct electron detector.

This study evaluates the Gatan Alpine detector at 100-200 keV for cryo-EM, showing improved resolution and performance compared to the Gatan K3.

An ultra-potent synthetic nanobody neutralizes SARS-CoV-2 by locking Spike into an inactive conformation

This paper develops a potent synthetic nanobody that neutralizes SARS-CoV-2 by locking its Spike protein in an inactive conformation, enabling aerosol delivery as a prophylactic or therapeutic agent.

Bivalent binding of a fully human IgG to the SARS-CoV-2 spike proteins reveals mechanisms of potent neutralization

This study identifies a potent SARS-CoV-2 neutralizing antibody, 5A6, that binds bivalently to two RBDs on the spike protein, showing resistance to key mutations.

Hedgehog pathway activation through conformational blockade of the Patched sterol conduit

This paper develops a nanobody agonist that activates the Hedgehog pathway by stabilizing an alternative conformation of Patched1, disrupting its sterol conduit and providing a new approach for transporter-related drug development.

A simple and robust procedure for preparing graphene-oxide cryo-EM grids

This paper describes a method for preparing graphene-oxide cryo-EM grids that are suitable for high-resolution single particle cryo-EM.