G. C. Lander

High-resolution single-particle imaging at 100-200 keV with the Gatan Alpine direct electron detector.

This study evaluates the Gatan Alpine detector at 100-200 keV for cryo-EM, showing improved resolution and performance compared to the Gatan K3.

The Pex6 N1 domain is required for Pex15 binding and proper assembly with Pex1

This study identifies the Pex6 N1 domain's role in binding Pex15 and stabilizing the Pex1/Pex6 heterohexamer, essential for peroxisome function.

Protein-mediated genome folding allosterically enhances site-specific integration of foreign DNA into CRISPRs

The paper determines the structure of a CRISPR integration complex and explains how it facilitates site-specific DNA integration through DNA bending and loop formation.

Succinylation of a KEAP1 sensor lysine promotes NRF2 activation

The paper identifies succinylation of KEAP1 lysine 131 as a mechanism to activate NRF2 transcriptional signaling through metabolic regulation.

Structure of the hepatitis C virus E1E2 glycoprotein complex

The paper presents the cryo-EM structure of the HCV E1E2 glycoprotein complex with antibodies, revealing the E1-E2 interface and its stabilization mechanisms.

Hidden conformations differentiate day and night in a circadian pacemaker

The paper uses cryo-EM to reveal structural changes in KaiC during day and night that regulate clock protein binding and circadian rhythms.

A case for glycerol as an acceptable additive to cryoEM samples

This study shows glycerol can be used as a cryoEM sample additive without compromising high-resolution structure determination, using mouse apoferritin and rabbit muscle aldolase as examples.

Sub-2 A Resolution Structure Determination Using Single-Particle Cryo-EM at 200 keV

This study shows that a 200 keV cryo-EM microscope can achieve 1.7 Å resolution for macromolecular structures, comparable to higher voltage systems.

Protein dynamics regulate distinct biochemical properties of cryptochromes in mammalian circadian rhythms

The paper identifies a dynamic loop in cryptochromes that regulates their differential binding to CLOCK:BMAL1, explaining their distinct roles in circadian rhythms.

Specific lid-base contacts in the 26S proteasome control the conformational switching required for substrate engagement and degradation

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

The paper demonstrates that conventional cryo-EM can determine high-resolution structures of sub-100 kDa complexes, resolving ligands and conformational states with sub-nanometer precision.

Conformational ensemble of the human TRPV3 ion channel

The paper presents cryo-EM structures of human TRPV3 in different states to elucidate its sensitization and gating mechanisms.

Insights into autophagosome biogenesis from structural and biochemical analyses of the ATG2A-WIPI4 complex

The paper characterizes the ATG2A-WIPI4 complex's role in autophagosome biogenesis through structural and biochemical analyses.

Achieving Better Than 3 A Resolution By Single Particle Cryo-EM At 200 keV

This paper shows that 200 keV TEMs can achieve cryo-EM resolutions better than 3 Å, enabling detailed structural analysis previously thought possible only with 300 keV TEMs.

A multi-model approach to assessing local and global cryo-EM map quality

The paper presents a multi-model approach to assess local and global cryo-EM map quality using independent model generation and convergence analysis.