W.-H. Lee

HIV envelope trimers and gp120 as immunogens to induce broadly neutralizing antibodies in cows

This study investigates whether engineering HIV Env trimers or using gp120 can induce broadly neutralizing antibodies in cows, finding that trimers are more effective than monomeric gp120.

LncRNA BRE-AS1 Regulates the JAK2/STAT3-mediated Inflammatory Activation via the miR-30b-5p/SOC3 Axis in THP-1 cells

This study identifies BRE-AS1's role in modulating inflammatory activation in THP-1 cells via the miR-30b-5p/SOC3/JAK2/STAT3 pathway, showing its impact on cytokine production and signaling.

Immunization of cows with HIV envelope trimers generates broadly neutralizing antibodies to the V2-apex from the ultralong CDRH3 repertoire

This study immunized cows with HIV Env trimers to generate broadly neutralizing antibodies targeting the V2-apex, revealing ultralong CDRH3 antibodies outcompete shorter ones in the response.

Broadly inhibitory antibodies against severe malaria virulence proteins

The paper identifies two human monoclonal antibodies that broadly inhibit PfEMP1's binding to EPCR, a key process in severe malaria pathogenesis.

Defining bottlenecks and opportunities for Lassa virus neutralization by structural profiling of vaccine-induced polyclonal antibody responses

This study identifies neutralizing antibody epitopes against Lassa virus GPC using electron microscopy, revealing challenges in vaccine-induced responses and opportunities for improved vaccine design.

Structure-guided mutagenesis of OSCAs reveals differential activation to mechanical stimuli

The paper identifies structural elements in OSCA channels that differentially mediate stretch and poking activation through mutagenesis and electrophysiology.

Germline-targeting chimpanzee SIV Envelopes induce V2-apex broadly neutralizing-like B cell precursors in a rhesus macaque infection model

This study shows that germline-targeting SCIVs can activate and expand B cells expressing V2-apex bnAb-like precursors in rhesus macaques.

Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines

The paper introduces a triple tandem trimer (TTT) design for HIV-1 Env and influenza HA immunogens, showing improved trimer-specific immune responses in viral vectored vaccines.

Identifying distinct neural features between the initial and corrective phases of precise reaching using AutoLFADS

This study uses AutoLFADS to analyze neural activity during initial and corrective movements in rhesus macaques, revealing distinct neural features and the limitations of traditional decoding methods.

Structure of mechanically activated ion channel OSCA2.3 reveals mobile elements in the transmembrane domain

The paper determines the structure of Arabidopsis thaliana OSCA2.3 using cryo-EM, revealing conformational heterogeneity and a conserved interaction between the TM6a-TM7 linker and the Beam-Like Domain.

Immunogenetic metabolomics revealed key enzymes that modulate CAR-T metabolism and function

The paper identifies ADA as a metabolic regulator that enhances CAR-T cell function and tumor clearance through improved metabolism and reduced exhaustion.

Broad SARS-CoV-2 Neutralization by Monoclonal and Bispecific Antibodies Derived from a Gamma-infected Individual

This study identifies broadly neutralizing monoclonal and bispecific antibodies from a Gamma variant-infected individual that effectively neutralize multiple SARS-CoV-2 variants.

Structural basis of epitope selectivity and potent protection from malaria by PfCSP antibody L9

The paper determines the cryo-EM structure of the anti-PfCSP antibody L9, revealing its multivalent binding to NPNV repeats and the role of the light chain in affinity and protective efficacy.

Affinity-matured homotypic interactions induce spectrum of PfCSP-antibody structures that influence protection from malaria infection

The paper analyzes structural and functional properties of antibodies targeting PfCSP, showing how homotypic interactions and affinity maturation influence protection against malaria.

Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus

This study identifies nAbs against SIVmac239, reveals structural features of its Env trimer, and shows passive transfer of nAbs protects macaques from infection.

Long-lasting germinal center responses to a priming immunization with continuous proliferation and somatic mutation

This study shows that germinal center B cells can persist and continue to mutate for 29 weeks after a single immunization, leading to long-lasting antibody responses.

Broadly neutralizing antibodies to SARS-related viruses can be readily induced in rhesus macaques

This study shows that rhesus macaques can be immunized to produce broadly neutralizing antibodies against multiple SARS-related viruses, highlighting differences in B cell repertoires between humans and macaques.

Structural Insights into the Venus flytrap Mechanosensitive Ion Channel Flycatcher1

This study characterizes the mechanosensitive ion channel FLYC1 using cryo-EM, MD simulations, and electrophysiology, revealing structural features and conformational transitions involved in mechano-gating.

Changes at V2 apex of HIV-1 Clade C trimer enhance elicitation of autologous neutralizing and broad V1V2-scaffold antibodies

Elicitation of potent serum neutralizing antibody responses in rabbits by immunization with an HIV-1 clade C trimeric Env derived from an Indian elite neutralizer

This study shows that immunization with a thermostable HIV-1 clade C Env trimer induces potent autologous neutralizing antibodies in rabbits, with distinct epitope specificity compared to natural infection.

Murine monoclonal antibodies against RBD of SARS-CoV-2 neutralize authentic wild type SARS-CoV-2 as well as B.1.1.7 and B.1.351 viruses and protect in vivo in a mouse model in a neutralization dependent manner

This study evaluates murine monoclonal antibodies against SARS-CoV-2 RBD, showing neutralization of wild-type and variants, and in vivo protection dependent on neutralization.

Bivalent binding of a fully human IgG to the SARS-CoV-2 spike proteins reveals mechanisms of potent neutralization

This study identifies a potent SARS-CoV-2 neutralizing antibody, 5A6, that binds bivalently to two RBDs on the spike protein, showing resistance to key mutations.

Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike

The paper modifies HIV-1 Env trimers to increase glycan occupancy and reduce artificial glycan holes, improving vaccine antigen design.

Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein

The paper identifies two immunodominant linear epitopes on the SARS-CoV-2 spike protein that are targets of potent neutralizing antibodies in convalescent patient sera.

Generation of dual specific bivalent BiTEs (dbBIspecific T-cell Engaging antibodies) for cellular immunotherapy

This paper describes the generation of dual-specific bivalent BiTES for cancer immunotherapy, showing they maintain tumor targeting and kill CEA+ cells in vitro and in vivo.

HIV-1 Envelope and MPER antibody structures in lipid assemblies

This study presents methods to incorporate full-length HIV-1 Env into lipid assemblies for structural analysis, revealing the quaternary epitope of an MPER antibody and Env tilting during neutralization.

De novo transcriptome sequence of Senna tora provides insights into anthraquinone biosynthesis

The paper reports the de novo transcriptome of Senna tora using RNA-Seq and Iso-Seq, identifies genes and transcription factors involved in anthraquinone biosynthesis, and shows differential expression across tissue development stages.

Structures of the Otopetrin Proton Channels Otop1 and Otop3

The paper reports cryo-EM structures of zebrafish Otop1 and chicken Otop3, revealing their dimeric architecture and potential proton conduction pathways.

Structure of the Human Volume Regulated Anion Channel

The paper reports the high-resolution cryo-EM structure of human SWELL1, revealing its trimer of dimers assembly and identifying a charged residue critical for pore function in VRAC.