D. Walker

Efficient and reproducible generation of human iPSC-derived cardiomyocytes using a stirred bioreactor

This paper describes a method to generate high-purity, scalable human iPSC-derived cardiomyocytes using a bioreactor, showing improved viability, function, and consistency compared to traditional methods.

Characterization of transcriptional heterogeneity and novel therapeutic targets using single cell RNA-sequencing of primary and circulating Ewing sarcoma cells.

This study uses single-cell RNA-sequencing to analyze transcriptional heterogeneity in Ewing sarcoma and identifies TSPAN8 as a novel therapeutic target.

Stratifying macrophages based on their infectious burden identifies novel host targets for intervention during Crohns disease associated adherent-invasive Escherichia coli infection

The paper identifies novel therapeutic targets for AIEC infection by stratifying macrophages based on bacterial load and showing that targeting these pathways reduces AIEC replication.

Herptile gut microbiomes: a natural system to study multi-kingdom interactions between filamentous fungi and bacteria

This paper investigates the gut microbiomes of herptiles, showing that Basidiobolus fungi are common and interact with bacteria, potentially shaping bacterial communities through horizontal gene transfer.

Dysregulation of N-terminal acetylation causes cardiac arrhythmia and cardiomyopathy

This study identifies a mutation in NAA10 causing cardiac arrhythmia and cardiomyopathy by disrupting N-terminal acetylation, and shows potential therapeutic interventions.

Mechanism of targeted killing of P. aeruginosa by pyocins SX1 and SX2

This study identifies two novel pyocins, SX1 and SX2, and elucidates their mechanisms of action and uptake pathways in P. aeruginosa.

Rapastinel Accelerates Loss of Withdrawal Signs after Repeated Morphine and Blunts Relapse to Conditioned Place Preference

This study shows rapastinel reduces opioid withdrawal symptoms and prevents relapse to morphine conditioned place preference in rats.

Physiological insight into the conserved properties of Caenorhabditis elegans acid-sensing DEG/ENaCs

The paper identifies acid-sensitive DEG/ENaC subunits in C. elegans using TEVC, revealing functional similarities to vertebrate ASICs and potential drug targets.

Yersinia pestis genomes reveal plague in Britain 4,000 years ago

The paper identifies Yersinia pestis genomes from 4,000-year-old British remains, showing plague in Britain earlier than previously documented.

Distinct roles for two Caenorhabditis elegans acid-sensing ion channels in an ultradian clock

The paper identifies two acid-sensing ion channels in C. elegans with distinct roles in regulating pH and Ca2+ oscillations in the intestine's ultradian clock.

Capturing in-field root system dynamics with the RootTracker

The paper introduces RootTracker, a device that measures root growth in the field, and finds that maize hybrids respond differently to drought by growing more and deeper roots.

FusB energises import across the outer membrane through direct interaction with its ferredoxin substrate

The paper identifies FusB as essential for ferredoxin import across the outer membrane, showing it interacts with both FusA and the ferredoxin substrate.

Pyocin efficacy in a murine model of Pseudomonas aeruginosa sepsis

This study evaluates pyocin efficacy in a murine model of Pseudomonas aeruginosa sepsis, finding both pyocins reduce mortality and show in vivo activity.

Pyocin S5 import into Pseudomonas aeruginosa reveals a generic mode of bacteriocin transport

The paper identifies the import mechanism of Pyocin S5 in Pseudomonas aeruginosa, revealing a generic pathway for TonB-dependent bacteriocins across Gram-negative bacteria.

Engineering bacteriocin-mediated resistance against plant pathogenic bacteria in plants

This paper demonstrates that expressing the bacteriocin putidacin L1 in plants provides effective resistance against Pseudomonas syringae and related pathogens.

A phylogeography of the second plague pandemic revealed through the analysis of historical Y. pestis genomes

This study analyzes historical Y. pestis genomes to trace the phylogeography of the second plague pandemic, revealing patterns of genetic diversity and virulence gene loss.

Adherence to the iDSI reference case among published cost-per-DALY averted studies

This study evaluates adherence to the iDSI reference case guidelines in cost-per-DALY averted studies from 2011 to 2017, finding improved reporting standards but no significant methodological improvement.

Galleria mellonella as an infection model for the multi-host pathogen Streptococcus agalactiae reflects hypervirulence of ST283

This paper shows that GBS ST283 is more virulent in G. mellonella larvae than other isolates, suggesting the model can study virulence and test anti-GBS strategies.

Genomic inversion drives small colony variant formation and increased virulence in P. aeruginosa

The paper identifies a genomic inversion as the mechanism driving small colony variant formation and increased virulence in P. aeruginosa during chronic infection.