E. Lerner

Upregulation of TNFR2 Precedes TOX Expression by Exhausted T cells and Restricts Antitumor and Antiviral Immunity

PEG-mCherry interactions beyond classical macromolecular crowding

This study investigates PEG-induced structural and dynamical changes in mCherry using simulations and experiments, revealing specific PEG-mCherry interactions beyond classical crowding effects.

Rapid and specific detection of single nanoparticles and viruses in microfluidic laminar flow via confocal fluorescence microscopy

This paper develops a flow virometer using confocal fluorescence microscopy to rapidly and specifically detect single nanoparticles and viruses in microfluidic laminar flow.

Multiparameter-based photosynthetic state transitions of single phytoplankton cells

This study tracks single phytoplankton cell photophysiological states using multi-parameter fluorescence analysis during diurnal cycles and light changes, revealing distinct subpopulations and transitions.

Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC)

The paper introduces Brick-MIC, an affordable, open-source 3D-printed microscopy platform that enables single-molecule and super-resolution imaging techniques.

FRET-sensitized acceptor emission localization (FRETsael) - nanometer localization of biomolecular interactions using fluorescence lifetime imaging

This paper introduces FRETsael, a method to localize biomolecular interactions with nanometer accuracy using fluorescence lifetime imaging, achieving 20-30 nm resolution in simulations and experimental samples.

Glioblastoma and other intracranial tumors elicit systemic sympathetic hyperactivity that limits immunotherapeutic responses

This study shows that intracranial tumors cause systemic sympathetic hyperactivity, impairing T cell function and limiting immunotherapy, and that beta-adrenergic blockade improves outcomes in mouse models and human patients.

Dissecting Mechanisms of Ligand Binding and Conformational Changes in the Glutamine-Binding Protein

This study investigates the ligand binding mechanism of GlnBP in E. coli, finding that conformational changes and ligand binding are correlated, and that the induced-fit mechanism is more consistent with the data than conformational selection.

Fluorescent protein lifetimes report increased local densities and phases of nuclear condensates during embryonic stem cell differentiation

The paper uses fluorescent protein lifetimes to measure local densities in nuclear condensates during mouse embryonic stem cell differentiation, finding increased homogeneity in HP1 condensates post-differentiation.

Structural and Dynamic Insights Into α-Synuclein Dimer Conformations

The paper identifies a compact, stable dimeric conformation of α-synuclein that may promote dityrosine linkage and is implicated in Parkinson's disease.

Evidence for a compact apo-σ70 conformation in vitro and in vivo

The paper identifies a compact apo-σ70 conformation in vitro and in vivo using CL-MS, suggesting it may be transcriptionally inactive and activated upon RNAP binding.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Identification and Quantification of Within-Burst Dynamics in Singly-Labeled Single-Molecule Fluorescence Lifetime Experiments

This paper introduces an analytical framework to quantify within-burst dynamics in single-molecule fluorescence lifetime experiments, demonstrating its application to -synuclein monomer structural dynamics.

Multi-parameter photon-by-photon hidden Markov modeling

This paper introduces multi-parameter H2MM to distinguish FRET dynamics from photophysical transitions in smFRET data, improving the analysis of biomolecular conformational changes.

The structural heterogeneity of α-synuclein is governed by several distinct subpopulations with interconversion times slower than milliseconds

This study maps the conformational space of α-synuclein monomer using simulations and experimental validations, revealing stable conformations with slow interconversion times.

High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins

This paper introduces a high-throughput smFRET method using multispot excitation and SPAD arrays to rapidly analyze nucleic acid and protein conformations and interactions.

Monte-Carlo Diffusion-Enhanced Photon Inference: Distance Distributions And Conformational Dynamics In single-Molecule FRET

This paper introduces MC-DEPI, a method to analyze smFRET data by accounting for distance fluctuations and photo-blinking, showing how these factors affect distance estimation and introducing a machine learning approach to decouple them.

Characterizing highly dynamic conformational states: the transcription bubble in RNAP-promoter open complex as an example

This study uses smFRET and hybrid modeling to identify two conformational states of the transcription bubble in bacterial RPo, supporting a role for sub-millisecond dynamics in transcription start site selection.

The effect of macromolecular crowding on single-round transcription by E. coli RNA polymerase

This study investigates how macromolecular crowding affects single-round transcription by E. coli RNA polymerase, finding unexpected enhancements in kinetics with large crowders.

Multispot single-molecule FRET: high-throughput analysis of freely diffusing molecules

The paper introduces a multispot confocal setup for high-throughput smFRET assays and validates its performance using bacterial RNA transcription kinetics.