G. O'Toole

DPR-MEDIATED H2O2 RESISTANCE CONTRIBUTES TO STREPTOCOCCI SURVIVAL IN A CYSTIC FIBROSIS AIRWAY MODEL SYSTEM

The paper identifies Dpr-mediated H2O2 resistance as critical for Streptococcus sanguinis survival in a polymicrobial CF airway model.

Reconstitution of a Biofilm Adhesin System from a Sulfate-Reducing Bacterium in Pseudomonas fluorescens

This study reconstitutes a biofilm adhesin system from Desulfovibrio vulgaris in Pseudomonas fluorescens, showing that DvhA's N-terminal fragment is regulated by DvhG/DvhD and c-di-GMP levels.

An In Vitro Medium for Modeling Gut Dysbiosis Associated with Cystic Fibrosis

This paper develops a novel in vitro culture medium, CF-MiPro, that mimics the CF gut environment and alters non-CF microbiomes to resemble CF-like profiles.

Transcriptional Profiling and Genetic Analysis of a Cystic Fibrosis Airway-Relevant Model Shows Asymmetric Responses to Growth in a Polymicrobial Community

The paper compares transcriptional responses of a polymicrobial cystic fibrosis airway community to monocultures under different growth conditions and identifies community-specific gene expression patterns.

The Regulator FleQ Post-Transcriptionally Regulates the Production of RTX Adhesins by Pseudomonas fluorescens

This paper identifies FleQ as a post-transcriptional regulator of LapA and MapA adhesin production in Pseudomonas fluorescens, showing that its absence reduces biofilm formation despite increased transcript levels.

Evidence for the Type IV Pili Retraction Motor PilT as a Component of the Surface Sensing System in Pseudomonas aeruginosa

This study shows that PilT, a Type IV pili retraction motor, is essential for surface sensing in Pseudomonas aeruginosa and mediates cAMP production through its interaction with PilJ.

Assessment of the Glycan-Binding Profile of Pseudomonas aeruginosa PAO1

This study identifies the glycan-binding preferences of Pseudomonas aeruginosa PAO1 using glycan arrays and exogenous glycans, showing a preference for monosaccharides over complex structures.

Predicting Clinical Outcomes in Infants With Cystic Fibrosis From Stool Microbiota using Random Forest Algorithms

This paper uses random forest algorithms to predict clinical outcomes in children with cystic fibrosis based on stool microbiota composition.

Community composition shapes microbial-specific phenotypes in a cystic fibrosis polymicrobial model system

This study develops a polymicrobial biofilm model of the CF lung to investigate community-specific antimicrobial resistance and identifies LasR loss-of-function as a driver of tobramycin tolerance through increased phenazine production.

Development of the Intestinal Microbiome in Cystic Fibrosis in Early Life

This study tracks the development of the gut microbiome in children with cystic fibrosis from birth to age 4, finding persistent dysbiosis and early signs of inflammatory bowel disease-associated microbiota.

Surface-Induced cAMP Signaling Requires Multiple Features of the Pseudomonas aeruginosa Type IV Pili

This study shows that Pseudomonas aeruginosa type IV pili must be retracted by the PilT motor to induce cAMP signaling, challenging a current model of how this signaling occurs.

Intestinal Bacteroides Modulates Systemic Inflammation and the Microbial Ecology in a Mouse Model of CF: Evidence for Propionate and other Short Chain Fatty Acids Reducing Systemic Inflammatory Cytokines

This study shows that Bacteroides in CF patients reduces systemic inflammation via propionate and alters microbial ecology in a mouse model of CF.

The Diguanylate Cyclase YfiN of Pseudomonas aeruginosa Regulates Biofilm Maintenance in Response to Peroxide

This study shows that YfiN, a diguanylate cyclase in P. aeruginosa, regulates biofilm maintenance by controlling c-di-GMP levels, motility, and viability under peroxide stress.

Differential surface competition and biofilm invasion strategies of Pseudomonas aeruginosa PA14 and PA01

This study compares the biofilm colonization strategies of Pseudomonas aeruginosa PA14 and PAO1, showing PAO1 outcompetes PA14 during initial colonization but PA14 excels in invading pre-formed biofilms and surviving starvation.

Mild CF Lung Disease is Associated with Bacterial Community Stability

The paper identifies five bacterial community types in CF patients and finds that community instability is linked to worse lung function outcomes.

Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition

The paper reveals the structure and function of CdrA in Pseudomonas aeruginosa biofilms, showing how it mediates cell-cell interactions and how targeting it can inhibit biofilm formation.

Fine tuning cyclic-di-GMP signaling in Pseudomonas aeruginosa using the type 4 pili alignment complex

This paper identifies PilO's role in modulating SadC activity to regulate cyclic-di-GMP levels and biofilm formation in Pseudomonas aeruginosa.

Pseudomonas aeruginosa uses c-di-GMP phosphodiesterases RmcA and MorA to regulate biofilm maintenance

This study identifies RmcA and MorA as c-di-GMP phosphodiesterases essential for Pseudomonas aeruginosa to maintain mature biofilms under nutrient limitation.

MapA, a second large RTX adhesin, contributes to biofilm formation by Pseudomonas fluorescens

The paper identifies MapA, a second RTX adhesin in Pseudomonas fluorescens, which contributes to biofilm formation under specific conditions, with regulation distinct from LapA.

Exogenous alginate protects Staphylococcus aureus from killing by Pseudomonas aeruginosa

This study shows that exogenous alginate protects S. aureus from P. aeruginosa killing in co-culture models by downregulating specific P. aeruginosa genes and pathways.

Pseudomonas aeruginosa enhances the efficacy of norfloxacin against Staphylococcus aureus biofilms

This study shows that Pseudomonas aeruginosa exoproducts enhance norfloxacin efficacy against Staphylococcus aureus biofilms by increasing drug uptake and reducing efflux.

Availability of Zinc Impacts Interactions Between Streptococcus sanguinis and Pseudomonas aeruginosa in Co-culture

This study shows that zinc availability affects the growth dynamics between Streptococcus sanguinis and Pseudomonas aeruginosa in co-culture, with lower zinc levels favoring Streptococcus and higher levels favoring Pseudomonas.

Pseudomonas aeruginosa increases the sensitivity of biofilm-grown Staphylococcus aureus to membrane-targeting antiseptics and antibiotics

This study shows Pseudomonas aeruginosa exoproducts enhance the effectiveness of antimicrobial agents against Staphylococcus aureus biofilms by increasing membrane permeability.

Interspecies signaling generates exploratory motility in Pseudomonas aeruginosa

This study shows Pseudomonas aeruginosa changes its motility behavior in response to signals from Staphylococcus aureus, leading to directed movement and colony invasion.

Metabolic Modeling of Cystic Fibrosis Airway Communities Predicts Mechanisms of Pathogen Dominance

The paper develops a metabolic model of CF airway communities to predict pathogen dominance mechanisms using 16S rRNA sequencing data from 75 CF patient samples.

Pseudomonas aeruginosa Can Inhibit Growth of Streptococcal Species via Siderophore Production

This study shows that Pseudomonas aeruginosa inhibits growth of Streptococcus species by producing siderophores that compete for iron.

Flagellar stators stimulate c-di-GMP production by Pseudomonas aeruginosa

This study shows that flagellar stators in Pseudomonas aeruginosa stimulate c-di-GMP production, creating a feedback loop that regulates motility and biofilm formation.

Ethanol decreases Pseudomonas aeruginosa flagellar motility through a cyclic-di-GMP- and stator-dependent pathway

This study shows ethanol reduces Pseudomonas aeruginosa motility by increasing c-di-GMP and involving stator and PilMNOP proteins.

Coopting the Lap system of Pseudomonas fluorescens to reversibly customize bacterial cell surfaces

This study engineers the Lap system of Pseudomonas fluorescens to reversibly display protein cargo on cell surfaces and control its release in response to c-di-GMP levels.

Ligand-Mediated Biofilm Formation via Enhanced Physical Interaction Between a Diguanylate Cyclase and Its Receptor

This paper shows that the DGC GcbC enhances c-di-GMP synthesis and biofilm formation through ligand-mediated interaction with its receptor LapD, with citrate increasing this interaction.