Author

J. J. Arnold

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 3 Microbiology· 3 Biochemistry· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Research Focus:host-pathogen & immune response· 5 Organism:virus· 3 Approach:biophysical modeling· 1 Research Focus:cell signaling & trafficking· 1 Research Focus:chromatin & epigenetic regulation· 1 Research Focus:drug response / drug discovery· 1 Organism:mouse· 1

Frequent Co-Authors

C. E. Cameron· 8 shared D. Dulin· 4 shared F. S. Papini· 3 shared M. Depken· 3 shared M. Seifert· 3 shared P. van Nies· 3 shared A. Woodman· 2 shared B. Canard· 2 shared

Activity by Year

2018-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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8 shared papersMicrobiology

C. E. Cameron

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Enteroviral 2C protein is an RNA-stimulated ATPase and uses a two-step mechanism for binding to RNA and ATP

C. Yeager, G. Carter, D. W. Gohara, N. H. Yennawar, E. J. Enemark, J. J. Arnold, C. E. Cameron

This study elucidates the biochemical mechanism of enteroviral 2C protein's ATPase activity, showing RNA stimulates ATP hydrolysis via a two-step binding mechanism.

bioRxiv · Sep 28, 2022Biochemistry

Interfering with nucleotide excision by the coronavirus 3'-to-5' exoribonuclease

R. Chinthapatla, M. S. Sotoudegan, T. Anderson, I. M. Moustafa, K. T. Passow, S. A. Kennelly, R. Moorthy, D. Dulin, J. Y. Feng, D. A. Harki, R. Kirchdoerfer, C. E. Cameron, J. J. Arnold

This study investigates how SARS-CoV-2's ExoN enzyme processes RNA and identifies modifications that block its activity, offering potential for new antiviral strategies.

bioRxiv · Aug 11, 2022Biochemistry

IRES-independent, eIF2A/eIF2D-dependent translation of the enterovirus genome

H. Kim, D. Aponte-Diaz, M. S. Sotoudegan, J. J. Arnold, C. E. Cameron

This study shows that enterovirus genome recombination depends on eIF2A/eIF2D-dependent translation, not IRES-mediated translation, and explains non-replicative recombination and RNA persistence.

bioRxiv · Jun 1, 2022Microbiology

The nucleotide addition cycle of the SARS-CoV-2 polymerase

S. C. Bera, M. Seifert, R. Kirchdoerfer, P. van Nies, Y. Wubulikasimu, S. Quack, F. S. Papini, J. J. Arnold, B. Canard, C. E. Cameron, M. Depken, D. Dulin

This study describes the mechanochemical behavior of the SARS-CoV-2 polymerase using magnetic tweezers, revealing three catalytic conformations and a thermal ratchet mechanism for nucleotide incorporation.

bioRxiv · Mar 27, 2021Biophysics

Inhibition of SARS-CoV-2 polymerase by nucleotide analogs: a single moleculeperspective

M. Seifert, S. C. Bera, P. van Nies, R. N. Kirchdoerfer, A. Shannon, T.-T.-N. Le, X. Meng, H. Xia, J. M. Wood, L. D. Harris, F. S. Papini, J. J. Arnold, S. C. Almo, T. L. Grove, P.-Y. Shi, , D. Dulin

This study investigates how nucleotide analogs like Remdesivir affect SARS-CoV-2 polymerase activity using single-molecule techniques, revealing backtracking rather than termination and evasion of ddhCTP.

bioRxiv · Aug 6, 2020 · PreprintBiophysics

Induced copy-back RNA synthesis as a novel therapeutic mechanism against RNA viruses

R. Janissen, A. Woodman, K.-M. Lee, I. Moustafa, F. Fitzgerald, P.-N. Huang, L. Kuijpers, A. L. Perkins, D. A. Harki, J. J. Arnold, B. Solano, S.-R. Shih, C. E. Cameron, N. H. Dekker

This study shows that a ribonucleotide analogue induces copy-back RNA synthesis, a new mechanism to combat RNA viruses by promoting defective viral genomes.

bioRxiv · Feb 12, 2020 · PreprintMicrobiology

Temperature controlled high-throughput magnetic tweezers show striking difference in activation energies of replicating viral RNA-dependent RNA polymerases.

M. Seifert, P. van Nies, F. S. Papini, J. J. Arnold, M. M. Poranen, C. E. Cameron, M. Depken, D. Dulin

This study uses temperature-controlled magnetic tweezers to compare replication kinetics of RdRps from three RNA viruses, finding temperature-dependent activation differences.

bioRxiv · Jan 15, 2020Biophysics

A speed-fidelity trade-off determines the mutation rate and virulence of an RNA virus

W. Fitzsimmons, R. J. Woods, J. T. McCrone, A. Woodman, J. J. Arnold, M. Yennawar, R. Evans, C. E. Cameron, A. S. Lauring

This study shows that RNA viruses evolve high mutation rates as a byproduct of selection for faster replication, not because of adaptive advantages.

bioRxiv · Apr 27, 2018Microbiology