M. Chami

Mitochondria alterations in fibroblasts from sporadic Alzheimer's disease (AD) patients correlate with AD-related clinical hallmarks

The paper examines mitochondrial alterations in fibroblasts from Alzheimer's disease patients and links them to clinical hallmarks and cognitive decline.

Molecular insights into atypical modes of beta-arrestin interaction with seven transmembrane receptors

This study presents cryo-EM structures and experimental data revealing how beta-arrestins interact with GPCRs, particularly those with phosphorylation in the third intracellular loop and intrinsically biased receptors.

Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution

This study presents cryo-EM structures of GPR109A-G-protein complexes with agonists and uses them to engineer biased agonists with reduced flushing effects.

Structural insights into ligand-recognition, activation, and signaling-bias at the complement C5a receptor, C5aR1

This study presents cryo-EM structures of C5aR1 bound to C5a and a synthetic peptide, revealing conformational changes and G-protein coupling mechanisms.

Structural snapshots uncover a lock-and-key type conserved activation mechanism of β-arrestins by GPCRs

The paper presents structural snapshots of β-arrestins interacting with GPCRs, revealing a conserved 'lock-and-key' activation mechanism through specific phosphorylation patterns.

Apo and ligand-bound high resolution Cryo-EM structures of the human Kv3.1 reveal a novel binding site for positive modulators

The paper determines high-resolution Cryo-EM structures of the human Kv3.1 channel, both apo and ligand-bound, revealing a novel binding site for positive modulators.

Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog

The paper determines the cryo-EM structures of ExbBSm and ExbB-ExbDSm complex, identifies key residues for function and interaction with TonB/HasB, and reveals the role of ExbBSm extension in heme acquisition.

Analysis of diverse eukaryotes suggests the existence of an ancestral 1 mitochondrial apparatus derived from the bacterial type II secretion system

The paper identifies homologues of bacterial T2SS components in mitochondria of diverse eukaryotes, suggesting an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.

Insight into Lipopolysaccharide Translocation by Cryo-EM structures of a LptDE Transporter in Complex with Pro-Macrobodies

The paper uses cryo-EM to determine structures of the LptDE transporter complex with Pro-Macrobodies, revealing partially and fully opened states of the transporter during LPS translocation.

Cryo-EM structural studies of the agonist complexed human TRPV4 ion-channel reveals novel structural rearrangements resulting in an open-conformation

This paper reports the cryo-EM structure of hTRPV4 in an open conformation induced by 4-PDD agonist, revealing molecular mechanisms of activation and providing a template for inhibitor design.

Retrieving High-Resolution Information from Disordered 2D Crystals by Single Particle Cryo-EM

The paper develops a method to improve resolution and resolve conformational heterogeneity in 2D crystals using single-particle refinement, achieving 4 Å resolution for the MloK1 potassium channel.

Image processing techniques for high-resolution structure determination from badly ordered 2D crystals

The paper develops image-processing algorithms to improve 3D structure determination from badly ordered 2D crystals, achieving resolution improvements to approximately 5 Å.