V. Singh

The olfactory receptor SNIF-1 mediates foraging for leucine-rich diets in Caenorhabditis elegans

This study identifies SNIF-1 as an olfactory receptor in C. elegans that mediates foraging for leucine-rich diets via the AWC neurons in response to isoamyl alcohol.

Insights into gene conversion and crossing-over processes from long-read sequencing of human, chimpanzee and gorilla testes and sperm

This study uses long-read sequencing to analyze gene conversion and crossover events in human, chimpanzee, and gorilla testes and sperm, revealing species-specific patterns and GC bias in gene conversion.

ATTRv-V30M Type A amyloid fibrils from the heart and nerves exhibit structural homogeneity.

This study determines the structure of ATTRv-V30M amyloid fibrils from cardiac and nerve tissues using cryo-EM, finding structural homogeneity across tissues when transthyretin originates from the liver.

Enhancing affinity of neutralizing SARS-CoV-2 nanobody through facile structure-guided mutations in CDRs

This study enhances the affinity of SARS-CoV-2 neutralizing nanobodies through structure-guided mutations in CDRs, resulting in improved neutralization activity against pseudovirus.

Altering the redox status of Chlamydia trachomatis directly impacts its developmental cycle progression

This study shows that altering the redox status of Chlamydia trachomatis affects its developmental cycle, with AhpC overexpression delaying differentiation and knockdown accelerating it.

(p)ppGpp buffers cell division when membrane fluidity decreases in Escherichia coli.

This study shows that (p)ppGpp regulates cell division in E. coli when membrane fluidity decreases due to reduced unsaturated fatty acid synthesis.

Rodent Gut Bacteria Coexisting with an Insect Gut Virus in Parasitic Cysts: Metagenomic Evidence of Microbial Translocation and Co-adaptation in Spatially-Confined Niches

This study identifies gut bacteria and an insect virus in parasitic cysts of vole mice, suggesting microbial translocation and adaptation in spatially confined environments.

Cryo-EM confirms a common fibril fold in the heart of four patients with ATTRwt amyloidosis

The paper uses cryo-EM to show that cardiac ATTRwt amyloid fibrils from four patients are structurally identical, supporting their consistent clinical presentation.

The Bacterial Quorum-Sensing Signal 2-Aminoacetophenone Rewires Immune Cell Bioenergetics through the PGC-1α/ERRα Axis to Mediate Tolerance to Infection

This study shows that the bacterial QS signal 2-AA modulates immune cell bioenergetics via the PGC-1α/ERRα axis, promoting immune tolerance and pathogen persistence.

Distinct impacts of each anti-anti-sigma factor ortholog of the chlamydial Rsb partner switching mechanism on development in Chlamydia trachomatis

This paper investigates the role of two anti-anti-sigma factors, RsbV1 and RsbV2, in the partner switching mechanism of Chlamydia trachomatis, showing that RsbV2 overexpression blocks secondary differentiation, reducing infectious EB production.

Low-intensity pulsed ultrasound stimulation (LIPUS) modulates microglial activation following intracortical microelectrode implantation.

This study shows that LIPUS reduces microglial activation and improves tissue-microelectrode integration after intracortical implantation.

Single-cell precision nanotechnology in vivo

This study demonstrates DNA origami can be directed in vivo to target immune cells and cancer metastases, showing potential for precision drug delivery.

Molecular insights into atypical modes of beta-arrestin interaction with seven transmembrane receptors

This study presents cryo-EM structures and experimental data revealing how beta-arrestins interact with GPCRs, particularly those with phosphorylation in the third intracellular loop and intrinsically biased receptors.

Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution

This study presents cryo-EM structures of GPR109A-G-protein complexes with agonists and uses them to engineer biased agonists with reduced flushing effects.

Characterization of human lightness discrimination thresholds for independent spectral variations

This study measures human lightness discrimination thresholds under varying scene reflectance and light intensity, finding that extrinsic variations linearly affect thresholds.

The basis of antigenic operon fragmentation in Bacteroidota and commensalism

The paper investigates antigenic operon fragmentation in Bacteroidota, finding that genomic insertions and natural selection drive operon fragmentation, affecting pro-inflammatory properties and commensalism.

Structure of mitoribosome reveals mechanism of mRNA binding, tRNA interactions with L1 stalk, roles of cofactors and rRNA modifications

The paper presents a high-resolution structure of the human mitoribosome, revealing mechanisms of mRNA binding, tRNA interactions, and roles of cofactors and rRNA modifications.

O-GlcNAc modification forces the formation of an α-Synuclein amyloid-strain with notably diminished seeding activity and pathology

This study shows that O-GlcNAc modification of α-Synuclein leads to amyloid fibrils with reduced seeding activity and pathology in Parkinson's disease models.

Structural insights into agonist-binding and activation of the human complement C3a receptor

This study presents structural insights into C3a-C3aR interactions and identifies a G-protein-biased agonist for the receptor.

A very-low carbohydrate content in a high-fat diet modifies the plasma metabolome and alleviates experimental atherosclerosis

This study shows that a very-low carbohydrate high-fat diet (KD) reduces atherosclerosis in mice by altering the plasma metabolome and reducing inflammation.

Structural insights into ligand-recognition, activation, and signaling-bias at the complement C5a receptor, C5aR1

This study presents cryo-EM structures of C5aR1 bound to C5a and a synthetic peptide, revealing conformational changes and G-protein coupling mechanisms.

Air-Liquid-Interface Reorganizes Membrane Lipid and Enhance Recruitment of Slc26a3 to Lipid-Rich Domains in Human Colon

The paper shows that air-liquid interface culture increases DRA expression and activity in Caco-2/BBe cells and colonoids by enhancing ceramide-rich platform formation and differentiation.

SLC26A3 (DRA) is stimulated in a synergistic, intracellular Ca2+ dependent manner by cAMP AND ATP in intestinal epithelial cells

The paper shows that forskolin and ATP synergistically increase DRA activity in intestinal epithelial cells through intracellular Ca2+ elevation, which is blocked by BAPTA-AM.

Examining Anxiety and Risk-taking in Healthy Male and Female Wistar Rats using Spatial and Temporal Analysis of Elevated Plus Maze

This study introduces two new parameters for analyzing anxiety and risk-taking in rats using the Elevated Plus Maze, revealing sex differences in spatial and temporal behavior.

Peyers Patch B cells undergo cell death via neutrophil-released toxic DNA following sterile tissue injury

The paper shows that sterile tissue injury causes B cell death in Peyers patches via neutrophil-released toxic DNA, leading to tissue shrinkage and reduced secretory IgA.

Structural snapshots uncover a lock-and-key type conserved activation mechanism of β-arrestins by GPCRs

The paper presents structural snapshots of β-arrestins interacting with GPCRs, revealing a conserved 'lock-and-key' activation mechanism through specific phosphorylation patterns.

Toroidal displacement of Klebsiella pneumoniae by Pseudomonas aeruginosa is a unique mechanism to avoid competition for iron

This study identifies a unique mechanism where Pseudomonas aeruginosa displaces Klebsiella pneumoniae using rhamnolipid biosurfactant under iron-limited conditions to reduce competition for iron.

CDKL5 regulates p62-mediated selective autophagy and host antiviral defense

This study identifies CDKL5 as a regulator of virophagy, showing it promotes p62-mediated autophagy of viral capsids and enhances antiviral defense.

Computational design of peptides to target Nav1.7 channel with high potency and selectivity for the treatment of pain

The paper designs potent and selective peptide inhibitors of human NaV1.7 channels using Rosetta, with two peptides showing sub-nanomolar IC50 and high selectivity over other sodium channels.

A ripening-associated ethylene response factor ERF.D7 activates ARF2 orthologs to regulate tomato fruit ripening

This study identifies SlERF.D7 as a ripening regulator in tomatoes that activates SlARF2A/B to control ripening by integrating auxin and ethylene signaling.