S. Mishra

Ca2+ binding to Esyt is required to modulate membrane contact site density in Drosophila photoreceptors

This study shows that Ca2+ binding to dEsyt modulates ER-PM MCS density in Drosophila photoreceptors, linking Ca2+ influx to signal transduction.

Conformational switch of a peptide provides a novel strategy to design peptide loaded porous organic polymer for Pyroptosis pathway mediated cancer therapy

This paper designs a peptide-loaded porous organic polymer that undergoes a conformational switch in cancer cells, triggering pyroptosis via membrane pore formation.

Caenorhabditis elegans model for in vivo screening of human gut microbiota mediated colonization and colonization resistance against Clostridioides difficile

This study uses C. elegans to screen human gut microbiota for colonization and resistance against C. difficile, finding 46% colonization success and 50% in vivo inhibition of C. difficile.

A genetically-encoded nanobody sensor reveals conformational diversity in beta-arrestins orchestrated by distinct seven transmembrane receptors

This study introduces a nanobody sensor to detect conformational changes in beta-arrestins upon GPCR activation, revealing receptor-specific conformational diversity.

Molecular basis of ligand promiscuity, structural mimicry, and atypical dimerization in the chemokine receptors

This study investigates ligand promiscuity and signaling bias in chemokine receptors by analyzing binding modes and determining cryo-EM structures of receptor-ligand complexes.

Analysing the distribution of SARS-CoV-2 infections in schools: integrating model predictions with real world observations

This paper develops an analytical model to predict SARS-CoV-2 outbreak sizes in schools by integrating model predictions with real-world infection data from Ontario, Canada.

Pyruvate dehydrogenase kinase expression profile is a biomarker for cancer sensitivity to dichloroacetate-mediated growth inhibition.

This study identifies PDK expression profiles as a biomarker for cancer sensitivity to DCA, showing that low PDK3 expression correlates with higher DCA sensitivity.

A novel glideosome-associated protein S14 coordinates sporozoite gliding motility and infectivity in mosquito and mammalian hosts

Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

The paper identifies viral RHIMs in bat RNA viruses that regulate host cell death through Z-RNA sensing and ZBP1-RIPK3 signaling, revealing species-specific effects on human and bat cells.

Autophagy protein Atg7 is essential and druggable for maintaining malaria parasite cellular homeostasis and organelle biogenesis

This study shows that Atg7 is essential for malaria parasite development and identifies compounds that inhibit its function, suggesting it as a potential drug target.

Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution

This study presents cryo-EM structures of GPR109A-G-protein complexes with agonists and uses them to engineer biased agonists with reduced flushing effects.

Loss of PI3-kinase activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis

This study shows that IPMK's PI3-kinase activity is essential for PDK1 membrane localization, which activates AKT and supports intestinal homeostasis and regeneration.

Nuclear growth and import can be uncoupled

The paper shows nuclear growth and import can be uncoupled, suggesting chromatin mechanical properties drive nuclear growth rather than import alone.

Deciphering drug response and phenotypic heterogeneity of cancer cells using gene ensembles of regulatory units defined by chromatin domains

The paper analyzes the relationship between TAD activity and drug response in cancer cells and patients, finding stronger correlations with TADs than individual genes.

Structural insights into ligand-recognition, activation, and signaling-bias at the complement C5a receptor, C5aR1

This study presents cryo-EM structures of C5aR1 bound to C5a and a synthetic peptide, revealing conformational changes and G-protein coupling mechanisms.

Genomic Sequencing from Sputum for Tuberculosis Disease Diagnosis, Lineage Determination and Drug Susceptibility Prediction

This study evaluates direct-from-sputum whole-genome sequencing for tuberculosis diagnosis, lineage determination, and drug susceptibility prediction using different sequencing technologies.

Overexpression of the plastidal pseudo-protease AtFtsHi3 confers drought tolerance without penalizing growth

This study shows overexpression of AtFtsHi3 in Arabidopsis improves drought tolerance without affecting growth by altering stomatal density and enhancing water use efficiency.

Integration of silicon chip microstructures for in-line microbial cell lysis in soft microfluidics

The paper develops a method to integrate silicon microstructures into soft microfluidic devices for in-line microbial cell lysis using piezoelectric actuation.

Cryo-EM-based structural insights into supramolecular assemblies of γ-Hemolysin from Staphylococcus aureus reveal the pore formation mechanism.

This study uses cryo-EM to reveal the structure of γ-Hemolysin supramolecular assemblies, showing pore formation and lipid interactions in Staphylococcus aureus.

Streptomycin and Pyridomycin drug molecules provide the most potential inhibitors against Rv3871 to cure Tuberculosis

This paper identifies streptomycin and pyridomycin as potential inhibitors of Rv3871, an ATPase involved in ESAT-6/CFP-10 secretion in Mycobacterium tuberculosis, aiming to treat tuberculosis.

Constitutive Inhibition of Transient Receptor Potential CanonicalType 6 (TRPC6) by O-GlcNAcylation at Threonine-221

This study identifies O-GlcNAcylation at Thr221 as a constitutive inhibitor of TRPC6 channel conductance and NFAT signaling, showing its conservation and functional importance across TRPC family members.

Rheological transition driven by matrix makes cancer spheroids resilient under confinement

This study shows that ovarian cancer spheroids with an ECM coat (blastuloids) are more resilient under confinement than those without (moruloids) due to a rheological transition driven by the ECM-induced lumen.

Elucidation of sub-cellular H2S metabolism in Solanum lycopersicum L. and its assessment under development and biotic stress

The paper investigates H2S metabolism in tomato under development and biotic stress using in silico analysis of 29 proteins and their sub-cellular localization.

The Alzheimer's gene SORL1 is a key regulator of endosomal traffic and recycling in human neurons

This study shows SORL1 regulates endosomal recycling in neurons by affecting APP, TRKB, and GLUA1 trafficking, with implications for Alzheimer's disease.

TGF--dependent Lymphoid Tissue Residency of Stem-like T cells Limits the Response to Tumor Vaccine

The paper identifies TGF-β-dependent differentiation of stem-like CD8+ T cells into tissue-resident memory T cells in tumor draining lymph nodes, which negatively correlates with tumor vaccine response.

Single Serine on TSC2 Exerts Biased Control over mTORC1 Activation by ERK1/2 but Not Akt

The paper shows that a single serine on TSC2 selectively controls mTORC1 activation by ERK1/2 but not Akt, with implications for pathological versus physiological mTOR signaling.

Chronological lifespan extension and nucleotide salvage inhibition in yeast by isonicotinamide supplementation

This study shows that isonicotinamide (INAM) extends yeast chronological lifespan by inhibiting nucleotide salvage pathways, leading to NAD+ accumulation and a hormetic stress response.

Estimating the conjugative transfer rate of antibiotic resistance genes: Effect of model structural errors

This study quantifies how structural model errors affect estimates of plasmid transfer rates in virtual mating experiments, highlighting the need to account for plasmid cost and lag phase for unbiased results.

SARS-CoV-2 B.1.617.2 Delta variant replication, sensitivity to neutralising antibodies and vaccine breakthrough

This study evaluates the replication, antibody sensitivity, and vaccine efficacy of the SARS-CoV-2 B.1.617.2 Delta variant compared to other variants and vaccine-elicited immunity.

Cryo-EM structure of an elusive pre-transport intermediate of the multidrug transporter BmrCD reveals modes of asymmetric drug binding

Cryo-EM and EPR analysis reveal cooperative substrate binding in BmrCD in an architecture primed for transport.