P. M. Gilbert

A cryopreservation strategy for myoblast storage in paper-based scaffolds for inter-laboratory studies of skeletal muscle health

This paper develops a cryopreservation method for myoblasts in paper-based scaffolds, enabling inter-laboratory studies of skeletal muscle health.

Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling

This study shows that fibronectin promotes stronger stiffness-dependent responses in human myoblasts compared to laminin and collagen, with softer substrates increasing nuclear wrinkling linked to proliferation.

Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues

The paper examines how iPSC-derived motor neurons affect the ultrastructure of bioengineered human skeletal muscle tissues, showing improved sarcomere organization with motor neuron co-culture.

mini-MEndR: A 96-well mixed species assay to co-evaluate human and mouse Pax7+ cell-mediated skeletal muscle repair

The paper introduces a 96-well mini-MEndR assay to co-evaluate human and mouse Pax7+ cell-mediated skeletal muscle repair, demonstrating its utility for drug screening and phenotypic analysis.

Dystrophin is a mechanical tension modulator

This study shows that dystrophin modulates baseline homeostatic tension in skeletal muscle, linking increased tissue tension to DMD pathogenesis.

Rescue of aged muscle stem cell quiescence defects by AKT inhibition revealed with a 3D biomimetic culture assay

This paper develops a 3D biomimetic culture assay to study muscle stem cell quiescence and shows that AKT inhibition rescues quiescence defects in aged cells.

A three-dimensional human adipocyte model of fatty acid-induced obesity

This paper develops a 3D in vitro model of human adipocytes to study fatty acid-induced obesity and its associated pathologies.

Electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues

This study analyzes electrophysiological properties of healthy and dystrophic 3D bioengineered skeletal muscle tissues, showing DMD features are replicated in the model and a block co-polymer improves membrane potential.

Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis

This study introduces a new chamber design for measuring both global and local tension in 3D muscle tissues, revealing early mechanical homeostasis during development.

Functional arrays of human pluripotent stem cell-derived cardiac microtissues

This paper develops a 96-well platform of cardiac microtissues to measure contractile force for drug screening and tissue engineering.

A 96-well culture platform enables longitudinal analyses of engineered human skeletal muscle microtissue strength

The paper introduces a 96-well platform for culturing human skeletal muscle microtissues to study their strength and response to drugs over time.

TGFβ signaling curbs cell fusion and muscle regeneration

This study shows that TGFβ signaling inhibits muscle cell fusion and regeneration, with inhibition promoting fusion and muscle repair.

A three-dimensional culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction and disease modeling

This paper introduces a 3D co-culture model of human skeletal muscle and motor neurons to study adult neuromuscular junction development and disease, showing increased receptor upregulation and disease modeling capability.

Muscle stem cell intramuscular delivery within hyaluronan methylcellulose improves engraftment efficiency and dispersion

This study shows that delivering muscle stem cells in a hyaluronan-methylcellulose hydrogel improves engraftment efficiency and donor cell dispersion in skeletal muscle.