T. Doerr

Determining the Youngs Modulus of the Bacterial Cell Envelope

The paper measures the Young's modulus of bacterial cell envelopes in E. coli, V. cholerae, and S. aureus using a microfluidic system and inverse finite element analysis, finding values ranging from 0.12 to 2.06 MPa.

Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure in Enterobacter cloacae

This study identifies three genetic factors in Enterobacter cloacae that collectively contribute to resistance against the antimicrobial peptide cecropin B, showing inoculum-dependent survival heterogeneity.

Novel role for peptidoglycan carboxypeptidases in maintaining the balance between bacterial cell wall synthesis and degradation

This study identifies DacA1 as a critical factor in balancing bacterial cell wall synthesis and degradation in Vibrio cholerae through genetic screens and functional analysis.

Disrupting central carbon metabolism increases antibiotic susceptibility in Vibrio cholerae

This study shows that disrupting central carbon metabolism in Vibrio cholerae increases antibiotic susceptibility by affecting cell envelope integrity and VxrAB signaling.

Mechanical stimuli activate gene expression via a cell envelope stress sensing pathway

This study shows mechanical stress in bacterial cell envelopes activates the VxrAB two-component system, promoting cell wall synthesis and homeostasis.

Peptidoglycan recycling contributes to outer membrane integrity and carbapenem tolerance in Acinetobacter baumannii

This study identifies peptidoglycan recycling as a mechanism contributing to outer membrane integrity and carbapenem tolerance in Acinetobacter baumannii.

Lytic transglycosylases mitigate periplasmic crowding by degrading soluble cell wall turnover products

This study identifies lytic transglycosylases (LTGs) as essential for preventing periplasmic crowding by degrading peptidoglycan turnover products, independent of peptidoglycan recycling.

Vibrio cholerae's mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and autoaggregation

This study identifies zinc starvation as an inducer of VSP-II gene expression in V. cholerae, revealing a transcriptional network involving VerA and AerB that drives autoaggregation and aerotaxis.

A multifaceted cellular damage repair and prevention pathway promotes high level tolerance to β-lactam antibiotics

This study shows that the VxrAB system in Vibrio cholerae reduces β-lactam antibiotic toxicity by managing iron levels and oxidative damage, enhancing bacterial tolerance.

Class A Penicillin-Binding Protein-mediated cell wall synthesis promotes structural integrity during peptidoglycan endopeptidase insufficiency

This study investigates the role of endopeptidases and class A PBPs in PG synthesis and structural integrity in V. cholerae, showing that aPBPs can compensate for EP insufficiency.

Structural basis of peptidoglycan endopeptidase regulation

This study solves the crystal structure of ShyA, a peptidoglycan endopeptidase, revealing a regulatory mechanism involving conformational changes that activate its catalytic activity.

Modulation of peptidoglycan synthesis by recycled cell wall tetrapeptides

This study identifies LdcV as a key enzyme in Vibrio cholerae that cleaves recycled murotetrapeptides, showing its role in maintaining cell wall integrity and synthesis by preventing toxic accumulation of tetrapeptide intermediates.