Y. Mao

Dimer-monomer transition defines a hyper-thermostable peptidoglycan hydrolase mined from bacterial proteome by lysin-derived antimicrobial peptide-primed screening

The paper identifies hyper-thermostable peptidoglycan hydrolases from bacterial proteomes using lysin-derived antimicrobial peptide screening and shows their potent bactericidal activity.

Stable centromere association of the yeast histone variant Cse4 requires its essential N-terminal domain

The paper investigates how the N-terminal domain of Cse4 stabilizes its association with centromeres in yeast, showing that OA and Scm3 independently stabilize Cse4 through their interactions with the END region.

SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma

This study identifies SMYD5 as a methyltransferase that modifies RPL40 K22me3, enhancing translation and promoting hepatocellular carcinoma.

Inhibition of serine racemase prevents diabetic retinopathy

This study shows that inhibiting serine racemase with L-ABH prevents diabetic retinopathy by protecting retinal cells and maintaining glucose homeostasis in diabetic mice.

HIF1α controls adipose tissue growth through metabolic rechanneling

This study shows that HIF1α is essential for adipose tissue growth under normal conditions and in response to high-fat diets, by rechanneling metabolic intermediates into lipid synthesis.

Protein S-glutathionylation confers cell resistance to ferroptosis

This study shows that protein S-glutathionylation protects cells from ferroptosis by maintaining ARF6 function and reducing labile iron pools.

A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage

The paper identifies a conserved linker protein from Chlorella that can phase separate Rubisco in Chlamydomonas and some plants, enabling cross-species pyrenoid formation.

A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells

This paper introduces a perfusion-independent method to isolate liver sinusoidal endothelial cells with high purity and throughput, comparable to perfusion-dependent methods.

New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis

The paper uses long-read sequencing to obtain complete metagenome-assembled genomes of anammox bacteria, revealing functional divergence and adaptive evolution through horizontal gene transfer and mobile genetic elements.

TAD Evolutionary and functional characterization reveals diversity in mammalian TAD boundary properties and function

The paper analyzes TAD boundaries across species to show evolutionary conservation and functional significance, finding ultraconserved boundaries have stronger insulation and CTCF binding.

Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation

The paper identifies start codon-associated ribosomal frameshifting (SCARF) as a mechanism for nutrient stress adaptation, revealing its role in amino acid recycling and selective mRNA translation.

Homeostatic control of an iron repressor in a GI tract resident

The paper shows that Hap43, an iron-responsive transcription factor, is degraded under high iron conditions in C. albicans, leading to antioxidant gene activation and enhanced gut colonization.

Investigation of a mathematical model describing global cancer growth and treatment: An inhomogeneous model based on the generalized logistic equation

This paper introduces an inhomogeneous mathematical model based on the generalized logistic equation to describe tumor growth and treatment, validated against clinical observations and theories of tumor heterogeneity.

A bioinformatics approach to systematically analyze the molecular patterns of monkeypox virus-host cell interactions

This study analyzes monkeypox virus-host cell interactions using transcriptome data to identify signaling pathways, biomarkers, and potential drug candidates.

Allosteric Regulation of Aptamer Affinity through Mechano-Chemical Coupling

This paper introduces a method to modulate aptamer affinity using a molecular clamp that destabilizes secondary structure through mechanical tension, demonstrating up to 65-fold affinity changes without prior structural knowledge.

Brassinosteroids signaling component SlBZR1 promotes fruit ripening in tomato

This study shows that brassinosteroids promote tomato fruit ripening by enhancing ethylene production and carotenoid accumulation through the SlBZR1 transcription factor.

S373P Mutation Stabilizes the Receptor-binding Domain of Spike Protein in Omicron and Promotes Binding

The paper investigates the effect of the S373P mutation on the stability and binding ability of the Omicron spike protein's RBD using AFM-SMFS and molecular dynamics simulations.

Evolutionary Principles of Bacterial Signaling Capacity and Complexity

This study examines the evolution of bacterial signaling complexity in Campylobacterota, showing increases during niche expansion and decreases during specialization.

Metagenomic and Metabolomic Analyses Reveal Synergistic Effects of Fecal Microbiota Transplantation and Anti-PD-1 Therapy on Treating Colorectal Cancer

This study shows that combining fecal microbiota transplantation with anti-PD-1 therapy improves survival and tumor control in mice, with specific gut bacteria and metabolites contributing to the enhanced efficacy.

Solution structure of the phosphatidylinositol 3-phosphate binding domain from the Legionella effector SetA

The paper reports the NMR solution structure of the SetA-CTD domain from Legionella pneumophila, revealing its PI(3)P binding mechanism and structural features important for membrane association.

ECS1 and ECS2 regulate polyspermy and suppress the formation of haploid plants by promoting double fertilization

This paper shows that ECS1 and ECS2 promote double fertilization and prevent haploid and triploid plant formation by regulating pollen tube block and synergid disintegration.

Adaptor linked K63 di-Ubiquitin activates Nedd4/Rsp5 E3 ligase

The paper shows that Art1, Art4, and Art5 undergo K63-linked di-ubiquitination by Rsp5, enhancing PM recruitment and cargo ubiquitination, while HECT domain exosite protects this modification from cleavage by Ubp2.

Mechanistic insights into dynamic mutual regulation of USP14 and proteasome

This study reveals the dynamic regulation of USP14 and proteasome through cryo-EM structures and time-resolved analysis, identifying three regulatory checkpoints in proteasome function.

Tissue homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics

This study identifies how fibroblast network mechanics regulate tissue tension and adaptation to immune challenge in lymph nodes.

The size of helical pitch is important for microtubule plus end dynamic instability

This study shows that helical pitch size determines microtubule plus end dynamic instability through stochastic simulations, finding that 1.5 dimer pitch optimizes growth and shrinkage phases.

Hidden dynamics of ubiquitin-mediated autoregulation of human 26S proteasome during substrate degradation

The paper uses cryo-EM and deep learning to analyze human 26S proteasome conformers, revealing dynamics of ubiquitin-mediated autoregulation during substrate degradation.

Condensation of Rubisco into a proto-pyrenoid in higher plant chloroplasts

This study shows that introducing algal EPYC1 and hybrid Rubisco into Arabidopsis chloroplasts leads to phase-separated Rubisco condensates with pyrenoid-like properties, enhancing CO2 fixation without impairing growth.

Mucin-type O-glycosylation Landscapes of SARS-CoV-2 Spike Proteins

This study identifies site-specific O-glycosylation patterns in SARS-CoV-2 spike proteins, revealing host-dependent variations and their potential implications for viral function and therapeutic development.

Arhopalus rusticus (Coleoptera: Cerambycidae): a new vector for pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae)

The paper investigates the ability of Arhopalus rusticus to transmit pine wood nematodes, finding that 12 beetles can transmit the nematodes through feeding on pine needles.

Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins

The paper characterizes site-specific N-glycosylation of SARS-CoV-2 spike proteins expressed in human and insect cells, revealing differences in glycan types and their implications for infection and vaccine development.